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Strain Detail Sheet

Strain Name:
C57BL/6J-IkbkbM1Btlr/Mmmh
Stock Number:
038233--TBD-
Citation ID:
RRID:MMRRC_038233--TBD-
Other Names:
Impaired
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

IkbkbM1Btlr
Name: inhibitor of kappaB kinase beta; mutation 1, Bruce Beutler
Synonyms: Impaired
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
Ikbkb
Name: inhibitor of kappaB kinase beta
Synonyms: IKK-beta, IKK-2, IKK2, IKK[b], IKKbeta
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 16150
HGNC: HGNC:5960
Homologene: 7782

Additional Resources
IMPC
Google
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PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced T to C transition at base pair 22,666,020 (v38) on chromosome 8, or base pair 40,566 in the GenBank genomic region NC_000074 for the Ikbkb gene, corresponding to residue 1,841 in the mRNA sequence NM_001159774 (variant 1) within exon 17 of 22 total exons and to residue 1,841 in the mRNA sequence NM_010546 (variant 2) within exon 17 of 21 total exons. The mutation results in an leucine (L) to proline (P) substitution at position 570 (L570P) in both isoforms of the inhibitor of kappa-B kinase-? (IKK-?; alternatively, IKK-2) protein.

Strain Description

Phenotype

Homozygous: Mice exhibited impaired IgE and IgG1 responses to the cysteine protease papain. Some mice also exhibited an increased viral titer in the liver after exposure to mouse cytomegalovirus (MCMV). Mice exhibited an increase in the CD4+ to CD8+ T cell ratio caused by a diminished frequency of peripheral CD8+ T cells in CD3 T cells after MCMV infection. Some mice also exhibited a reduced frequency of B1a cells in B1 cells and an increased frequency of B1b cells in B1 cells in the peripheral blood after MCMV infection.

Heterozygous: Mice exhibited impaired IgE and IgG1 responses to the cysteine protease papain. Mice exhibited an increase in the CD4+ to CD8+ T cell ratio caused by a diminished frequency of peripheral CD8+ T cells in CD3 T cells after MCMV infection. Some mice also exhibited a reduced frequency of B1a cells in B1 cells and an increased frequency of B1b cells in B1 cells in the peripheral blood after MCMV infection. Values intermediate between wild-type and homozygous Impaired mice.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • immune system
  • hematopoietic system
    • abnormal CD8-positive, alpha-beta T cell differentiation [MP:0008080]
    • abnormal B cell physiology [MP:0002459]
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Immunology and Inflammation
  • Models for Human Disease
    • Immunodeficiency 15 (IMD15; OMIM: #615592)

Strain Origin

Primary Reference

SoRelle J, Misawa T, Chen Z, Murray AR, Beutler B. Record for Impaired. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

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Fees

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