Strain Detail Sheet

Strain Name:
C57BL/6J-Dock2m5Btlr/Mmmh
Stock Number:
038242--TBD-
Citation ID:
RRID:MMRRC_038242--TBD-
Other Names:
Arches
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Dock2m5Btlr
Name: dedicator of cyto-kinesis 2; mutation 5, Bruce Beutler
Synonyms: Arches
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
Dock2
Name: dedicator of cyto-kinesis 2
Synonyms: CED-5, MBC, Hch
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 94176
HGNC: HGNC:2988
Homologene: 37984
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Genetic Alterations
ENU-induced T to A transversion at base pair 34,689,760 (v38) on chromosome 11, or base pair 94,146 in the GenBank genomic region NC_000077 for the Dock2 gene. The mutation corresponds to residue 2,064 in the mRNA sequence NM_033374 within exon 20 of 52 total exons. The mutation results in a methionine (M) to lysine (K) substitution at position 661 (M661K) in the DOCK2 protein.

Strain Description

Phenotype

Homozygous: Mice showed an increased frequency of B1 cells as well as a decrease in the CD4+ to CD8+ T cell ratio caused by a diminished frequency of CD4+ T cells in CD3+ T cells coupled with an increased frequency of CD8+ T cells in CD3+ T cells, all in the peripheral blood.

Heterozygous: Mice showed a decrease in the CD4+ to CD8+ T cell ratio caused by a diminished frequency of CD4+ T cells in CD3+ T cells coupled with an increased frequency of CD8+ T cells in CD3+ T cells, all in the peripheral blood.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • hematopoietic system
    • abnormal CD4-positive T cell differentiation [MP:0008076]
    • abnormal CD8-positive, alpha-beta T cell differentiation [MP:0008080]
    • impaired hematopoiesis [MP:0001606]
    • decreased CD4-positive, alpha beta T cell number [MP:0008075]
    • increased CD8-positive, alpha-beta T cell number [MP:0008078]
    • decreased single-positive T cell number [MP:0008083]
    • increased single-positive T cell number [MP:0008082]
  • immune system
    • abnormal CD4-positive T cell differentiation [MP:0008076]
    • abnormal CD8-positive, alpha-beta T cell differentiation [MP:0008080]
    • decreased CD4-positive, alpha beta T cell number [MP:0008075]
    • increased CD8-positive, alpha-beta T cell number [MP:0008078]
    • decreased single-positive T cell number [MP:0008083]
    • increased single-positive T cell number [MP:0008082]
Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact . Older strains may not have this information.

Research Applications

  • Cell Biology
  • Immunology and Inflammation

Strain Origin

Primary Reference

Zeng M, Zhong X, Murray AR, Beutler B. Record for Arches. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable:
Homozygotes are fertile:
Heterozygotes are fertile:
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

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Fees

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