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Strain Detail Sheet

Strain Name:
C57BL/6J-Nlrp3M7Btlr/Mmmh
Stock Number:
038252--TBD-
Citation ID:
RRID:MMRRC_038252--TBD-
Other Names:
Park4
Major Collection:
Beutler Mutagenetix

Strain Information

Gene Details

Nlrp3M7Btlr
Name: NLR family, pyrin domain containing 3; mutation 7, Bruce Beutler
Synonyms: Park4
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
Nlrp3
Name: NLR family, pyrin domain containing 3
Synonyms: NALP3, Pypaf1, Cias1, Mmig1, cryopyrin
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216799
Homologene: 3600

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
ENU-induced A to G transition at base pair 59,549,531 (v38) on chromosome 11, or base pair 7,963 in the GenBank genomic region NC_000077 for the Nlrp3 gene. The mutation corresponds to residue 2,159 in the mRNA sequence NM_145827 within exon 4 of 10 total exons. The mutation results in a methionine (M) to valine (V) substitution at position 645 (M645V) in the NLRP3 protein

Strain Description

Phenotype

Homozygous: Mice exhibited attenuated inflammatory responses related to decrease secretion of the proinflammatory cytokine interleukin (IL)-1β in response to priming with lipopolysaccharide (LPS) followed by nigericin treatment.

Heterozygous: Mice exhibited attenuated inflammatory responses related to decrease secretion of the proinflammatory cytokine interleukin (IL)-1β in response to priming with lipopolysaccharide (LPS) followed by nigericin treatment intermediate phenotype between wild-type and homozygous Park3 mice.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )
  • immune system
    • abnormal macrophage cytokine production [MP:0011072]
    • decreased interleukin-1 beta secretion [MP:0008658]
  • hematopoietic system
    • abnormal macrophage cytokine production [MP:0011072]
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Immunology and Inflammation

Strain Origin

Primary Reference

Shi H, Wang Y, Zhang Z, Dao D, Sun L, Murray AR, Beutler B. Record for Park4. MUTAGENETIX, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Strain Development
Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact

Appearance

Coat Color
Black

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown

Order Request Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

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Fees

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