Strain Name:
C57BL/6N-Atm1Brd Slc7a2tm1a(EUCOMM)Hmgu/BayMmucd
Stock Number:
041537-UCD
Citation ID:
RRID:MMRRC_041537-UCD
Other Names:
C57BL/6N-Atm1Brd/a Slc7a2tm1a(EUCOMM)Hmgu/BcmMmucd
Major Collection:

Strain Information

Slc7a2tm1a(EUCOMM)Hmgu
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 2; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout-first
Slc7a2
Name: solute carrier family 7 (cationic amino acid transporter, y+ system), member 2
Synonyms: Tea, Atrc2, Cat2
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Knockout-first
NCBI: 11988
Homologene: 20659
Atm1Brd
Name: nonagouti; targeted mutation 1, Allan Bradley
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Targeted Mutation
a
Name: nonagouti
Synonyms: agouti, As, agouti signal protein, ASP
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Targeted Mutation
NCBI: 50518
HGNC: HGNC:745
Homologene: 1264
Genetic Alterations
The L1L2_Bact_P cassette was inserted at position 40902146 of Chromosome 8 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 40902908. The critical exon(s) is/are thus flanked by loxP sites.

Allele Detail page

KOMP Targeting Strategies

Genotype Determination
ES Cell Line
JM8A3.N1 derived from C57BL/6N
Phenotype
Phenotyping data may be available at mousephenotype.org.
Mammalian Phenotype Terms
Allelic Composition: Atm1Brd/Atm1Brd (Genetic Background: C57BL/6N-Atm1Brd )

Allelic Composition: Atm1Brd/a (Genetic Background: C57BL/6N-Atm1Brd )

MeSH Terms
  • Alleles
  • Animals
  • Computational Biology
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Gene Deletion
  • Gene Knockout Techniques/methods
  • Genes/genetics
  • Genes, Lethal/genetics
  • Genetic Association Studies/methods
  • Genetic Vectors/genetics
  • Genome/genetics
  • Genomics
  • Genotype
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout/genetics
  • Mutagenesis, Insertional/methods
  • Phenotype
  • Polymerase Chain Reaction
  • Rats
Strain Development
ES cell clone HEPD0823_8_B06 was injected into C57BL/6J-Tyrc-Brd blastocysts. Resulting chimeras were mated to C57BL/6N, progeny were subsequently mated to C57BL/6N.
Suggested Control Mice
Wild-type littermate or C57BL/6N mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
Donor
Arthur Beaudet, M.D., Baylor College of Medicine.
Mice were generated at the Baylor College of Medicine as part of the Baylor College of Medicine, Sanger Institute, and MRC Harwell (BaSH) Consortium for the NIH Common Fund program for Knockout Mouse Production and Cryopreservation (1U42RR033192-01) and Knockout Mouse Phenotyping (1U54HG006348-01).
Primary Reference
Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A. A conditional knockout resource for the genome-wide study of mouse gene function. Nature. 2011 Jun 15;474(7351):337-42. (Medline PMID: 21677750)

Colony and Husbandry Information


The MMRRC has recently been alerted that a translocation of the manipulated agouti allele was discovered via FISH analysis on chromosome 8 of ES cell line JM8A3, and potentially also affects the sub-lines JM8A3.N1 and JM8A1.N3, that were all derived at the Wellcome Trust Sanger Institute. This MMRRC distributed, EUCOMM allele, mouse line, donated from IMPC production centers, was derived from JM8A3, JM8A3.N1 or JM8A1.N3 ES cell lines, and is targeting Chromosome 8. Therefore, we recommend crossing to C57BL/6N wild type mice, in order to breed out the agouti coat color, and select black mice for continued breeding. For further information or assistance please contact Allan Bradley at The Sanger Institute abradley@sanger.ac.uk.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the BaSH EUCOMM Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041537-UCD-RESUS Litter recovered from cryo-archive $5,055.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.