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Strain Name:
C57BL/6N-Atm1Brd Glg1tm1a(KOMP)Wtsi/Mmucd
Stock Number:
047864-UCD
Citation ID:
RRID:MMRRC_047864-UCD
Other Names:
Glg1_C07, CSD47433
Major Collection:

Strain Information

Glg1tm1a(KOMP)Wtsi
Name: golgi apparatus protein 1; targeted mutation 1a, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout-first
Glg1
Name: golgi apparatus protein 1
Synonyms: ESL-1, CFR, MG-160, MG160, CFR-1, Selel
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Knockout-first
NCBI: 20340
HGNC: HGNC:4316
Homologene: 7533
Atm1Brd
Name: nonagouti; targeted mutation 1, Allan Bradley
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Targeted Mutation
Genetic Alterations

Knockout First(Promoter driven)

**ALERT regarding wild type JM8A3, JM8A3.N1 or JM8A1.N3 parental cells:
The KOMP Project has recently identified the presence of a translocation of the manipulated agouti allele on chromosome 8 of ES cell line JM8A3, and potentially affecting the sub-lines JM8A3.N1 and JM8A1.N3, that were all derived at the Wellcome Trust Sanger Institute. At this time, we recommended not to use these cells for targeting genes on chromosome 8. Other lines, including, the JM8, JM8.N4, or JM8.F6, do not exhibit this translocation and can be safely and reliably used for gene targeting experiments. If you are using mouse lines (e.g., KOMP targeted lines) derived from JM8A3, JM8A3.N1 or JM8A1.N3 ES cell lines, we recommend crossing to C57BL/6N wild type mice in order to breed out the agouti coat color. For further information or assistance please contact Allan Bradley at The Sanger Institute abradley@sanger.ac.uk.

KOMP Targeting Strategies

Genotype Determination
ES Cell Line
JM8A1.N3 derived from C57BL/6N
Phenotype
Phenotyping data may be available at mousephenotype.org.
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

  • hematopoietic system
    • increased erythrocyte cell number []
  • homeostasis/metabolism
    • increased circulating thyroxine level []
    • increased circulating glycerol level []
Allelic Composition: Atm1Brd/Atm1Brd (Genetic Background: C57BL/6N-Atm1Brd )

Allelic Composition: Atm1Brd/a (Genetic Background: C57BL/6N-Atm1Brd )

MeSH Terms
  • Alleles
  • Animals
  • Computational Biology
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Gene Deletion
  • Gene Knockout Techniques/methods
  • Genes/genetics
  • Genes, Lethal/genetics
  • Genetic Association Studies/methods
  • Genetic Vectors/genetics
  • Genome/genetics
  • Genomics
  • Genotype
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout/genetics
  • Mutagenesis, Insertional/methods
  • Phenotype
  • Polymerase Chain Reaction
  • Rats
Strain Development
ES cell clone EPD0269_2_C07 was injected into morulae or blastocysts. Resulting chimeras were mated to C57BL/6N mice and heterozygous tm1a (Knockout First) animals were generated.The MMRRC will use C57BL/6N females for cryo-recovery.
Suggested Control Mice
C57BL/6N or wild-type from colony
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
Donor
The KOMP Repository, University of California, Davis; Originating from Ramiro Ramirez-Solis, CSD
Primary Reference
Skarnes, W.C., Rosen, B., West, A.P., Koutsourakis, M., Bushell, W., Iyer, V., Mujica, A.O., Thomas, M., Harrow, J., Cox, T. et al. (2011) A conditional knockout resource for the genome-wide study of mouse gene function. Nature, 474, 337-342. (PMID: 21677750)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
black or agouti
MMRRC Breeding System
Backcross or Sib-Mating

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the KOMP MTA. The MTA should be signed by an authorized official from your institution, generally this is someone in your Technology Transfer or Intellectual Property offices. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
047864-UCD-RESUS Litter recovered from cryo-archive $5,055.00 / $8,533.74
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.