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Availability & Fees Order this Strain
The ZEN-UB1 Velocigene cassette (beta-galactosidase coding sequence from E. coli lacZ gene; polyadenylation signal; loxP site; promoter from the human ubiquitin C gene; neomycin phosphotransferase;polyadenylation signal; loxP site) was inserted into the gene replacing all coding exons and intervening sequences.
CREed Deletion
Further details at: http://velocigene.com/komp/detail/11217
KOMP Targeting Strategies
The construct was introduced into C57BL/6NTac-derived VGB6 embryonic stem (ES) cells, and correctly targeted ES cells (clone 11217A-F1) were injected into B6(Cg)-Tyrc-2J/J (RRID:IMSR_JAX:000058) blastocysts. The resulting chimeric males were bred to C57BL/6NJ (RRID:IMSR_JAX:005304) females to generate heterozygous tm1 (Deletion) animals. The tm1.1 (CREed Deletion) allele was produced by a cross to a ubiquitous Cre deleter mouse (B6N.Cg-Tg(Sox2-cre)1Amc/J, RRID:IMSR_JAX:014094) for recombination of the loxP sites which also removed the neo cassette. The resulting offspring were crossed to remove the cre-expressing transgene. The archived germplasm does not contain the Cre transgene. The MMRRC will use C57BL/6N females for cryo-recovery.
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the KOMP MTA. The MTA should be signed by an authorized official from your institution, generally this is someone in your Technology Transfer or Intellectual Property offices. A link to this form will be provided via email when an order is submitted; the PDF form should be downloaded or the email forwarded to the appropriate institutional representative for completion following directions in the document.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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