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Strain Detail Sheet

Strain Name:
ES cell line Fbxo2tm1(cre/ERT2)Hels/Mmucd
Stock Number:
050514-UCD
Citation ID:
RRID:MMRRC_050514-UCD
Other Names:
Fbxo2-VHC

Gene Information

Gene Details

Fbxo2tm1(cre/ERT2)Hels
Name: F-box protein 2; targeted mutation 1, Stefan Heller
Type: Allele
Species: Multi-species
Chromosome: 4
Alteration at locus: Knock-In
MGI:
cre/ERT2
Name: cre tamoxifen-dependent recombinase
Type: Gene
Species: Multi-species
Chromosome:
Alteration at locus: Knock-In
Fbxo2
Name: F-box protein 2
Synonyms: NFB42, FBX2, Fbs1, Prpl4
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Knock-In
NCBI: 230904
Homologene: 8132
Genetic Alterations
The following was inserted to the start site of Fbxo2, exon 1: H2B-Venus_P2A_HygroR_P2A_CreERt2_polyA_loxP-Neo-loxP. The allele is identical to MGI:6194328, albeit without to cre exision (i.e., tm1).
Genotype Determination
Genotyping Protocol
ES Cell Line
E14TG2a.4 derived from 129P2/OlaHsd

Strain Description

Strain of Origin
129P2/OlaHsd
Strain Development
ES cells were electroporated with linearized targeting vector as described in PMID:30179592. G418 selection, clonal expansion, and screening via multiple long-range PCR assays.

Strain Origin

Donor
Stefan Heller, Ph.D Leland Stanford Junior University School of Medicine
Primary Reference

Hartman BH, Bӧscke R, Ellwanger DC, Keymeulen S, Scheibinger M, Heller S.Fbxo2(VHC) mouse and embryonic stem cell reporter lines delineate invitro-generated inner ear sensory epithelia cells and enable otic lineageselection and Cre-recombination. Dev Biol. 2018 Sep 1. pii:S0012-1606(18)30499-8. doi: 10.1016/j.ydbio.2018.08.013. [Epub ahead of print](Medline PMID: 30179592)

Colony and Husbandry Information

Special Considerations

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Health Status Report

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Order Request Information

Conditions of Distribution

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.