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Strain Detail Sheet

Published: 11/30/2023
Revised: 12/14/2025

Strain Name:
C57BL/6J-Akt3em1Shzb/Mmjax
Stock Number:
065431-JAX
Citation ID:
RRID:MMRRC_065431-JAX
Other Names:
Akt3fl/fl

Strain Information

Gene Details

Akt3em1Shzb
Name: Akt serine/threonine kinase 3; endonuclease-mediated mutation 1, Bridget Shafit-Zagardo
Synonyms: Akt3fl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: CRISPR
Akt3
Name: Akt serine/threonine kinase 3
Synonyms: PKB gamma, D930002M15Rik, Nmf350
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23797
HGNC: HGNC:393
Homologene: 55904

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A pair of gRNAs targeting to intron 2 and intron 3 of Akt3 respectively were designed by an online tool (http://crispr.mit.edu/) and generated by in vitro transcription (PMID:23992847). Cas9 mRNA was purchased from SBI. Akt3 conditional knockout HRD plasmid containing the 2kb homologous arms at each side and the floxed exon 3 was generated by SLiCE cloning (PMID:22241772).
Genotype Determination
ES Cell Line
Not applicable

Strain Description

Phenotype
None/Normal/Wild-type

Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Immunology and Inflammation

Strain Origin

Submitter
Bridget Shafit-Zagardo, Ph.D., Albert Einstein College of Medicine.
Primary Reference

DuBois JC, Ray AK, Gruber RC, Zhang Y, Aflakpui R, Macian-Juan F,Shafit-Zagardo B. Akt3-Mediated Protection Against Inflammatory DemyelinatingDisease. Front Immunol. 2019 Jul 25;10:1738. doi: 10.3389/fimmu.2019.01738.eCollection 2019. (Medline PMID: 31404142)

Strain Development
Superovulated female C57BL/6J mice (3–4 weeks old) were mated to C57BL/6J males, and fertilized embryos were collected from oviducts. A pair of in vitro transcribed guide RNAs (gRNAs) targeting to intron 2 and intron 3 of Akt3, Cas9 mRNA and Akt3 conditional knockout HRD were mixed and microinjected into the cytoplasm of fertilized eggs. The injected zygotes were transferred into pseudopregnant CD1 females, and the resulting pups were obtained. A male Akt3 fl/+ mouse was obtained from the resulting pregnancy. The founder male mouse was mated with 5 WT females from separate mating lines; the resulting offspring were mated to generate Akt3 fl/fl mice.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

For more information about this colony's health status contact csmmrrc@jax.org

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Sib-Mating
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Information

Availability Level

The availability level for this product has not been determined.

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact Office of Biotechnology and Business Development.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact Office of Biotechnology and Business Development

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.