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Strain Detail Sheet

Published: 12/17/2020
Revised: 05/07/2026

Strain Name:
C57BL/6-Megf11tm1a(EUCOMM)Wtsi/JnkMmnc
Stock Number:
068042-UNC
Citation ID:
RRID:MMRRC_068042-UNC
Other Names:
Megf11tm1a(EUCOMM)Wtsi, B6J.Cg-Megf11tm1a(EUCOMM)Wtsi/JnkMmnc
GQC Report Thumbnail
MMRRC Strain GQC Report

Strain Information

Strain Quality Control

QUALITY CONTROL OF C57BL/6-Megf11tm1a(EUCOMM)Wtsi/JnkMmnc  |  University of North Carolina at Chapel Hill
Last Updated:
Strain Data and Information Information by Submitter Assessed by MMRRC1,2
Published Provided
Allele-specific genotype3  YES
Genetic background  YES
Viability of genotypes available for distribution  YES
Specific Pathogen- Status  YES
Recoverability of cryopreserved sperm/embryos4  YES
Gene or allele sequence3  n.d.
Gene or allele expression3  n.d.
Gene or allele function3  n.d.
Observable and/or measurable phenotypes  n.d.
Fertility of genotypes available for distribution  YES
Fecundity/breeding performance  YES

1 When indicated as "assessed by MMRRC" ("YES"), please note that the information presented on this strain is to the best of our knowledge correct and up-to-date at the the MMRRC Distribution Center assigned to maintain and distribute this strain. This information is subject to change due to breeding, maintenance, and other actions. Please direct any questions on the information presented in this table to the MMRRC Distribution Center.

2 If an assessment has not been performed by the MMRRC (as indicated by "NO"), investigators may request specific testing for a fee. Requests should be submitted directly to the MMRRC Distribution Center assigned to the management, archiving, and distribution of the strain. A full listing of available testing and analytical services is available at https://www.mmrrc.org/about/services.php.

3 This information may or may not apply to each individual engineered allele (e.g., Cre, FlpO) present in the strain.

4 Recovery refers to thawing, in vitro fertilization (IVF), and/or embryo culture leading to live offspring.

Gene Details

Megf11tm1a(EUCOMM)Wtsi
Name: multiple EGF-like-domains 11; targeted mutation 1a, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout-first
Megf11
Name: multiple EGF-like-domains 11
Synonyms: 2410080H04Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 214058
Homologene: 13031

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
The L1L2_Bact_P cassette was inserted at position 64508113 of Chromosome 9 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 64509096. The critical exon(s) is/are thus flanked by loxP sites.

A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    JM8A3.N1

    Strain Description

    Phenotype
    • Homozygous KO: Moderately abnormal horizontal cell morphology In the retina.
    • No phenotype in the heterozygous KOs.
    • A "conditional-ready" floxed allele, tm1c, can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, the tm1b allele will be created, which turns the mouse into a reporter knockout.
    MeSH Terms
    • Amacrine Cells/cytology
    • Amacrine Cells/metabolism
    • Animals
    • Cell Adhesion
    • Gene Expression Regulation
    • HEK293 Cells
    • Humans
    • Ligands
    • Membrane Proteins/deficiency
    • Membrane Proteins/genetics
    • Membrane Proteins/metabolism
    • Mice
    • Mice, Inbred C57BL
    • Mutation
    • Photoreceptor Cells, Vertebrate/metabolism
    • Retinal Horizontal Cells/cytology
    • Retinal Horizontal Cells/metabolism
    Strain GQC Summary
    Congenic strain carrying the tm1a(EUCOMM)Wtsi allele of the Megf11 gene on C57BL/6J background. The allele of interest present on chromosome 9 is on the donor background (C57BL/6NCrl). C57BL/6J was detected with diagnostic alleles. The genome of this strain is fully replicable. This GQC report is based on the MiniMUGA G3 2024 pipeline using 2 samples genotyped in 2021.
    MMRRC Strain GQC Report
    Suggested Control Mice
    Littermates of all relevant genotypes.

    Research Applications

    • Neurobiology

    Strain Origin

    Submitter
    Joshua Sanes, Ph.D., Harvard University.
    Jeremy N. Kay, Ph.D., Duke University Medical Center.
    Primary Reference

    Kay JN, Chu MW, Sanes JR. MEGF10 and MEGF11 mediate homotypic interactions required for mosaic spacing of retinal neurons. Nature. 2012 Mar 11;483(7390):465-9. doi: 10.1038/nature10877. (Medline PMID: 22407321)

    Strain Development

    Three independent ES cell clones (C57BL/6N) carrying the targeted mutation were obtained from EUCOMM. Two of these successfully produced chimeric mice. Male chimeras were bred to B6(Cg)-Tyrc-2J/J (RRID:IMSR_JAX:000058) mice to establish germline transmission. Since 2010, maintained by C57BL/6J backcrossing (N10+) prior to its archival at the MMRRC.


    Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Backcross and sib-mating
    Generation
    N10 (C57BL/6J), F5
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 10 to 12 months
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    7 to 9

    Order Information

    Availability Level

    The availability level for this product has not been determined.

    Conditions of Distribution

    The submitter or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    - Products for this strain are Not Yet Available for Ordering
    - If you register interest in this strain, you will be notified when it becomes available for ordering.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.