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Strain Detail Sheet

Published: 02/22/2022
Revised: 04/08/2022

Strain Name:
B6NCrl.129(Cg)-Rapgef4tm1.2Chen/ChengMmucd
Stock Number:
069574-UCD
Citation ID:
RRID:MMRRC_069574-UCD
Other Names:
Epac2 total KO [Rapgef4tm1.2Chen (MGI:5571845)]

Strain Information

Gene Details

Rapgef4tm1.2Chen
Name: Rap guanine nucleotide exchange factor (GEF) 4; targeted mutation 1.2, Ju Chen
Synonyms: Epac2-KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knockout
Rapgef4
Name: Rap guanine nucleotide exchange factor (GEF) 4
Synonyms: Epac2, cAMP-GEFII, 1300003D15Rik, 5730402K07Rik, 6330581N18Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 56508
Homologene: 4451

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations

A loxP site was inserted upstream of exon 9 (ENSMUSE00001293030). An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted downstream of exon 9. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exon 9.

ES Cell Line
R1

Strain Description

Phenotype
Epac2 knockout mice were born at the expected Mendelian ratios, viable and fertile, and developmentally normal without obvious abnormality. Epac2-/- mice developed polyuria (PMID:30252533).
MeSH Terms
  • Animals
  • Arrhythmias, Cardiac/complications
  • Arrhythmias, Cardiac/metabolism
  • Arrhythmias, Cardiac/physiopathology
  • Calcium/deficiency
  • Calcium/metabolism
  • Calcium Signaling/physiology
  • Guanine Nucleotide Exchange Factors/deficiency
  • Guanine Nucleotide Exchange Factors/genetics
  • Guanine Nucleotide Exchange Factors/physiology
  • Heart Failure/etiology
  • Heart Failure/metabolism
  • Mice
  • Mice, Knockout
  • Myocytes, Cardiac/metabolism
  • Myocytes, Cardiac/pathology
  • Receptors, Adrenergic, beta-1/physiology
  • Sarcoplasmic Reticulum/metabolism
  • Sarcoplasmic Reticulum/pathology
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Diabetes
  • Endocrine Deficiency
  • Hematology
  • Immunology and Inflammation
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Reproduction
  • Research Tools
  • Virology

Strain Origin

Submitter
Ju Chen, Ph.D., University of California, San Diego School of Medicine.
Primary Reference

Pereira L, Cheng H, Lao DH, Na L, van Oort RJ, Brown JH, Wehrens XH, Chen J, Bers DM. Epac2 mediates cardiac β1-adrenergic-dependent sarcoplasmic reticulum Ca2+ leak and arrhythmia. Circulation. 2013 Feb 26;127(8):913-22. doi: 10.1161/CIRCULATIONAHA.12.148619. Epub 2013 Jan 30. (Medline PMID: 23363625)

Additional References

Cherezova A, Tomilin V, Buncha V, Zaika O, Ortiz PA, Mei F, Cheng X, Mamenko M, Pochynyuk O. Urinary concentrating defect in mice lacking Epac1 or Epac2. FASEB J. 2019 Feb;33(2):2156-2170. doi: 10.1096/fj.201800435R. Epub 2018 Sep 25. (Medline PMID: 30252533)

Strain Development
R1 embryonic stem cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+ were used for homologous recombination and implanted into C57BL/6 blastocysts. Male chimeras were mated with female black Swiss mice. The selection cassette was deleted by mating to B6.Cg-Tg(ACTFLPe)9205Dym/J (RRID:IMSR_JAX:005703), and exon 9 was excised by breeding to 129S/Sv-Tg(Prm-cre)58Og/J (RRID:IMSR_JAX:003328, which was crossed with C57BL/6 for >5 generations). Subsequent backcrossing to C57BL/6NCrl for >20 generations.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross and sib-mating
Generation
N20+ (C57BL/6NCrl), F2+
Overall Breeding Performance
Poor

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.