Strain Name:
C57BL/6J-Gt(ROSA)26Sorem8(CAG-Venus*,-Katushka2S)Murr/MurrMmjax
Stock Number:
069711-JAX
Citation ID:
RRID:MMRRC_069711-JAX
Other Names:
C57BL/6J-Gt(ROSA)26Sor^tm8(CAG-Venus*,-Katushka2S)Murr/MurrMmjax; Traffic Light Reporter 7

Strain Information

C57BL/6J-Gt(ROSA)26Sorem8(CAG-Venus*,-Katushka2S)Murr
Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 8, Stephen Murray
Type: Allele
Species: Mus musculus
Chromosome: 6
Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: beta geo, R26, Gtrosa26, Thumpd3as1, Gtrgeo26, ROSA26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14910
ES Cell Line
Not applicable
Phenotype
The TLR-7 "traffic light reporter" line is designed to detect homology directed repair (HDR) or non-homologous end joining (NHEJ) repair events in the same allele. A CAG promoter directs expression of a mutant (non-functional) Venus (GFP/YFP variant) and a Katushka2S (red) fluorescent protein in the endogenous Gt(ROSA)26Sor locus. A 30bp insert containing an sgRNA target sequence replaces 3 codons of the Venus protein, disrupting its function. The Katushka2S gene is encoded in the +3 frame, and therefore neither Venus nor Katushka2S are expressed in the allele's native state. When a double-strand break is generated at the target region (nuclease cleavage site), Venus expression can be restored via HDR when an appropriate DNA donor is provided, while NHEJ events that result in a -2 frame shift will result in Katushka2S expression. No fluorescent protein expression is observed prior to CRISPR-mediated targeting of the reporter region. Homozygotes are viable and fertile.
Strain Development
This strain was developed by inserting the following construct into the second intron of the Gt(ROSA)26Sor locus of C57BL/6J via Bxb1 mediated integration at landing sites engineered into strainC57BL/6J-Gt(ROSA)26Sorem7Mvw/Mvw: CMV Enhancer, Chicken Beta-actin promoter and chimeric intron, followed by Venus (GFP) coding sequence interrupted by the insertion of 30 bases and deletion of sequence codons 95-97, and P2A peptide. Katushka2S (RFP) follows the P2A peptide in the +3 reading frame and bovine growth hormone polyA termination sequence. The targeting construct was microinjected into oocytes from C57BL/6J-Gt(ROSA)26Sorem7Mvw/Mvw, along with Bxb1 integrase mRNA to generate founders with properly targeted sequences to the Rosa26 locus. Mice were backcrossed to C57BL/6J for at least 2 generations and then intercrossed to generate homozygotes.
Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
  • Research Tools
Donor
Stephen Murray, Ph.D., The Jackson Laboratory.
Primary Reference
Not ready to publish

Colony and Husbandry Information

For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Undetermined
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
4 Weeks
Average Pups Weaned
Undetermined

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069711-JAX-HOM-F
069711-JAX-HOM-M
Homozygous Female
Homozygous Male
$218.00 / $218.00
Non-Profit / For-Profit
Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.