Strain Name:
C57BL/6N-Gt(ROSA)26Sorem3(CAG-Venus*)Rkuhn/MurrMmjax
Stock Number:
069724-JAX
Citation ID:
RRID:MMRRC_069724-JAX
Other Names:
C57BL/6N-Gt(ROSA)26Sor^em3(CAG-Venus*)Rkuhn/MurrMmjax; R26-GFP_KI-ABE

Strain Information

Gt(ROSA)26Sor
Name: gene trap ROSA 26, Philippe Soriano
Synonyms: beta geo, R26, Gtrosa26, Thumpd3as1, Gtrgeo26, ROSA26
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14910
Genetic Alterations
R26-GFP_KI-ABE (Adenine Base Editor) reporter knock-in mice express a CAG promoter controlling expression of a non-functional Venus (GFP) reporter that can be reverted using an A base editor to activate GFP. This strain may be useful for reporting out CRISPR-targeting base editors (specifically the A base editor).
ES Cell Line
Not applicable
Phenotype
R26-GFP_KI-ABE (Adenine Base Editor) reporter knock-in mice were created using CRISPR/cas9 genome editing to insert a CAG promoter controlling expression of a non-functional Venus (GFP) reporter (with a stop engineered at codon 81) in the endogenous Gt(ROSA)26Sor locus. When an ABE together with a guide RNA that binds at the mutant site is introduced into cells, the stop is converted into a glutamine codon and the reporter will express GFP. Homozygotes are viable and fertile. As the mice are characterized, we will modify the strain-description and add phenotype data. This strain may be useful for reporting out CRISPR-targeting base editors (specifically the A base editor).
Strain Development
The R26-GFP_KI-ABE (Adenine Base Editor reporter) targeting vector was designed to insert the ABE reporter construct into the Gt(ROSA)26Sor locus containing a CAG promoter, mutated Venus*, bovine hGH, and polyA sequence. Venus* is a non-functional reporter that has codon 81 mutated into a stop codon. The targeting construct was microinjected into zygotes of C57BL/6N mice together with cas9 protein and a guide RNA creating a DNA double-strand break in the Rosa26 locus. then transferred to pseudopregnant females. Progeny was screened by DNA sequencing of the targeted region and a founder was identified as harboring the desired allele. This founder was bred to C57BL/6N for germline transmission. The colony was backcrossed to C57BL/6N for two generations. Upon arrival, sperm was cryopreserved. To establish our live colony, an aliquot of frozen sperm was used to fertilize C57BL/6NJ oocytes.
Suggested Control Mice
C57BL/6N
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
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Donor
Ralf Kuehn, Ph.D., Max-Delbrück-Centrum for Molecular Medicine.
Primary Reference
Not ready to publish

Colony and Husbandry Information

For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
N2
Overall Breeding Performance
Undetermined
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
4 Weeks
Average Pups Weaned
Undetermined

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Gred Müller.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Gred Müller

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069724-JAX-HOM-F
069724-JAX-HOM-M
Homozygous Female
Homozygous Male
$218.00 / $218.00
Non-Profit / For-Profit
Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.