Strain Name:
C57BL/6J-Stmn2em8(STMN2*)Lutzy/Mmjax
Stock Number:
069792-JAX
Citation ID:
RRID:MMRRC_069792-JAX
Other Names:
C57BL/6J-Stmn2^em8(STMN2*)Lutzy/Mmjax

Strain Information

Stmn2em8Lutzy
Name: stathmin-like 2; endonuclease-mediated mutation, 8th time this PI used the technique, Cat Lutz
Type: Allele
Species: Mus musculus
Chromosome: 3
Stmn2
Name: stathmin-like 2
Synonyms: Scgn10, SCG10
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20257
Homologene: 5102
Genetic Alterations
Stmn2em8(STMN2*) is a CRISPR/cas9 generated mutant of the stathmin-like 2 (Stmn2) gene carrying 222 nt of human STMN2 exon 2a. The STMN2 sequence is modified to include the human MS2 stem loop sequence and replace the TDP43 binding element. These mice may be useful in pre-clinical studies of Amyotrophic Lateral Sclerosis (ALS).
Genotype Determination
ES Cell Line
Not applicable
Phenotype
Stmn1 (stathmin-like 2) encodes a phosphoprotein that is involved in microtubule dynamics and signal transduction. Specifically, it is involved in the regulation of neuronal growth. The Stmn2em8 knockin mice were created using CRISPR/cas9 genome editing to insert 222 nt of the human STMN2 exon 2a and flanking regions into intron 1 of the mouse Stmn2 gene. The STMN2 sequence is modified to include the human MS2 stem-loop sequence and replace the TDP43 binding element. The targeted Stmn2 gene encodes a protein involved in microtubule stability. Mice heterozygous for the mutation are viable and fertile. There are fewer homozygotes (11.8%) produced from a heterozygote x heterozygote mating than expected, suggesting a subviable homozygous phenotype with either embryonic or early perinatal loss. As the mice are characterized, we will modify the strain-description and add phenotype data.
Strain Development
The Stmn2em8(STMN2*) allele was generated using CRISPR/cas9 endonuclease-mediated genome editing. Guide RNAs were selected to target intron 1 of the stathmin-like 2 gene (Stmn2) and insert a modified human SMNT2 exon 2a. The humanized/mutant exon 2a in this strain is 222 nt in length and is flanked by 1491 bp and 1548 bp of human genomic DNA, located 5’- and 3’- of exon 2a, respectively. The 19-nt MS2 stem loop contains the sequence 5’-ACATGAGGATCACCCATGT-3’ was used to replace the 24-nt TDP-43 binding sequence 5’-TGTGTGAGCATGTGTGCGTGTGTG-3’ in the 3’-UTR of the exon2a-containing mouse-human hybrid STMN2 prematurely terminated mRNA transcript. The donor plasmid, both guides and cas9 nuclease were introduced into single cell C57BL/6J zygotes and transferred to pseudopregnant females. Note that the flanking mouse intron 1 sequences present in the donor plasmid was derived from N2A cell line. There are three SNPs in the left arm that differ between the C57BL/6J sequence reported in Ensembl. It is not expected that any of these SNPs impact the usage of exon 2a. Progeny were screened by DNA sequencing to identify correctly targeted pups, which were then bred to C57BL/6J mice (JAXStock No. 000664) for germline transmission. N1 progeny derived from a single correctly targeted founder carrying the allele were backcrossed to C57BL/6J for at least two generations to establish the colony.
Suggested Control Mice
C57BL/6J
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
Donor
Cat Lutz, Ph.D., The Jackson Laboratory.
Primary Reference
Not ready to publish

Colony and Husbandry Information

For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
N2 (C57BL/6J)
Overall Breeding Performance
Undetermined
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Undetermined
Recommended wean age
4 Weeks
Average Pups Weaned
Undetermined

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
069792-JAX-HET-F
069792-JAX-HET-M
069792-JAX-WT-F
069792-JAX-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
$218.00 / $218.00
Non-Profit / For-Profit
Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.