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Strain Detail Sheet

Strain Name:
CC005.129S6-Is(19BORCS7-AS3MT)1Bhk/Mmnc
Stock Number:
071633-UNC
Citation ID:
RRID:MMRRC_071633-UNC
Other Names:
CC005.129S6-hAS3MT

Strain Information

Gene Details

Is(19BORCS7-AS3MT)1Bhk
Name: insertion, Chr 19, Beverly H Koller 1; insertion, Chr 19, Beverly H Koller 1
Type: Allele
Species: Multi-species
Chromosome: 19
Alteration at locus: Transgenic
As3mt
Name: arsenite methyltransferase
Synonyms: Cyt19, 2310045H08Rik
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Transgenic
NCBI: 57344
VEGA: 19
Homologene: 10754

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Borcs7
Name: BLOC-1 related complex subunit 7
Synonyms: 2010012O05Rik
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Transgenic
NCBI: 66439
VEGA: 19
Homologene: 16941

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
AS3MT
Name: arsenite methyltransferase
Type: Gene
Species: Homo sapiens (human)
Chromosome: 10
Alteration at locus: Transgenic
BORCS7
Name: BLOC-1 related complex subunit 7
Type: Gene
Species: Homo sapiens (human)
Chromosome: 10
Alteration at locus: Transgenic
Genetic Alterations
The targeting construct replaced the 60,908 bp segment extending from chr19:46,683,032 to 46,744,011 with a 59,261-bp segment of human DNA extending from chr10:102,845,563 to 102,904,823 and a floxed neomycin resistance cassette.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Unspecified

    Strain Description

    Phenotype
    None/Normal/Wild-type

    Conditional phenotype: no


    Strain Development
    The Borcs7/As3mt displacer construct was assembled using a standard recombineering approach. The mouse arms of homology were derived from the 129s7/AB2.2 bMQ BAC library. The short homology arm was derived from BAC bMQ455L14 and the long arm of homology from BAC bMQ345L20. The segment of human genomic DNA containing the BORCS7 and AS3MT loci was derived from the human tile path BAC RP11-753C18 (BACPAC Resources Center). A DNA segment (60,908 bp) extending from chr19:46,683,032 to 46,744,011 were deleted from the mouse genome and replaced with a 59,261-bp segment of human DNA extending from chr10:102,845,563 to 102,904,823. The haplotype of the AS3MT gene carried in the displacer construct is *1A (Wood et al. 2006). The resistance marker gene used for selection of ES cells in which the displacer construct underwent genomic integration consisted of a phosphoglycerate kinase (PGK)-neo cassette flanked by mutant loxP sites. The marker gene can be excised, leaving only a nonfunctional lox site in its place. This line is congenic and has been backcrossed for at least 10 generations.
    Suggested Control Mice
    wild-type

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Models for Human Disease

    Strain Origin

    Donor
    Fernando Pardo Manuel De Villena, Ph.D., University of North Carolina-Chapel Hill.
    Primary Reference
    In preparation, submitted, or in press

    Colony and Husbandry Information

    Special Considerations

    none

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Appearance

    Coat Color
    Agouti
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Backcross
    Generation
    n11
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Undetermined Undetermined
    Average litter size
    4 to 6
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    4 to 6

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@med.unc.edu for more details.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Conditions of Distribution

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    071633-UNC-SPERM Cryo-preserved spermatozoa $520.00 / $863.00
    Non-Profit / For-Profit    		 Aliquot Approximate quantity3
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.