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Strain Detail Sheet
Strain Name:
hACE2/hTMPRSS2/Tyk2_P1124A
Stock Number:
071889-UCD
Citation ID:
RRID:MMRRC_071889-UCD
Other Names:
hACE2/hTMPRSS2/Tyk2_P1124A
Major Collection:
Ace2em1(ACE2)Mbp
Name: angiotensin converting enzyme 2; endonuclease-mediated mutation 1, Mouse Biology Program, UC Davis
Type: Allele
Species: Multi-species
Chromosome: X
Alteration at locus: CRISPR
MGI: MGI:6856620
Tmprss2em1(TMPRSS2)Mbp
Name: transmembrane protease, serine 2; endonuclease-mediated mutation 1, Mouse Biology Program, UC Davis
Type: Allele
Species: Multi-species
Chromosome: 16
Alteration at locus: CRISPR
MGI: MGI:6856764
Ace2
Name: angiotensin converting enzyme 2
Synonyms: 2010305L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: CRISPR
MGI: MGI:1917258
NCBI: 70008
Ensembl: ENSMUSG00000015405
VEGA: OTTMUSG00000019518
HGNC: HGNC:13557
Homologene: 41448
CCDS: CCDS30518.1
UCSC: ChrX:162922338-162971414
Additional Resources
Tmprss2
Name: transmembrane protease, serine 2
Synonyms: epitheliasin, D16Ertd61e
Type: Gene
Species: Mouse
Chromosome: 16
Alteration at locus: CRISPR
MGI: MGI:1354381
NCBI: 50528
Ensembl: ENSMUSG00000000385
VEGA: 16
HGNC: HGNC:11876
Homologene: 4136
CCDS: CCDS37417.1
UCSC: Chr16:97365882-97460741
Additional Resources
Tyk2
Name: tyrosine kinase 2
Synonyms: JTK1
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: CRISPR
MGI: MGI:1929470
NCBI: 54721
Ensembl: ENSMUSG00000032175
VEGA: 9
HGNC: HGNC:12440
Homologene: 20712
CCDS: CCDS57656.1, CCDS90510.1
UCSC: Chr9:21015364-21042689
Additional Resources
Genetic Alterations
Tyk2_P1124A was created in the mouse to model the human coding variant TYK2_P1104A caused by rs34536443. CRISPR RNP was used to assist with homology directed repair (HDR) using an ssODN repair template electroporated in mouse C57BL/6N-Ace2em1(ACE2)Mbp Tmprss2em1(TMPRSS2)Mbp zygotes. The targeted residue codon CCC to GCC at the 1124th residue as well as two silent mutations were engineered into the HDR template totaling 3 mismatches to prevent RNP re-cleavage. Founder animals were screened by QPCR MGB probe with positives subsequently PCR’d and bidirectionally sequenced at the loci.
Genotype Determination
ES Cell Line
Not applicable
Phenotype
Unknown
Strain Development
Tyk2_P1124A was created in the mouse to model the human coding variant TYK2_P1104A caused by rs34536443. CRISPR RNP was used to assist with homology directed repair (HDR) using an ssODN repair template electroporated in mouse C57BL/6N-Ace2em1(ACE2)Mbp Tmprss2em1(TMPRSS2)Mbp zygotes. The targeted residue codon CCC to GCC at the 1124th residue as well as two silent mutations were engineered into the HDR template totaling 3 mismatches to prevent RNP re-cleavage. Founder animals were screened by QPCR MGB probe with positives subsequently PCR’d and bidirectionally sequenced at the loci. Sequence confirmed founder animals were then backcrossed to C57BL/6N-Ace2em1(ACE2)Mbp Tmprss2em1(TMPRSS2)Mbp and subsequence N1 heterozygous animals were confirmed for copy number as well as sequence analysis. Potential off targets with an MIT score greater than 1.5 were not found.
Suggested Control Mice
C57BL/6NCrl or wild-type siblings
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
- Immunology and Inflammation
- Models for Human Disease
Donor
Kent Lloyd, D.V.M., University of California, Davis.
Primary Reference
Not ready to publish
Colony Surveillance Program and Current Health Reports
For more information about this colony's health status contact mmrrc@ucdavis.eduCoat Color
Black
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
Unknown
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
| Viability and Fertility: | Female | Male | Comments |
|---|---|---|---|
| Homozygotes are viable: | Yes | X-linked | |
| Homozygotes are fertile: | Yes | X-linked | |
| Hetero/Hemizygotes are fertile: | Yes | Yes | |
| Age Reproductive Decline: | Undetermined | Undetermined |
Average litter size
4 to 6
Recommended wean age
4 Weeks
Average Pups Weaned
4 to 6
The availability level for this product has not been determined.
The donor or their institution limits the distribution to non-profit institutions only.
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