Human TRAF6 DNA consisting of CDS (exons 3-17) fused to mouse Traf6 3'UTR was inserted into exon 3 beginning at the 15th coding residue of mouse Nrp1 gene. This approach minimizes potential downstream splicing or RNP re-cleavage as the guide protospacer was split. Expression of the human CDS is primarily controlled by the mouse regulatory system and is expected to not transcribe the mouse version. CRISPR RNP was used to assist with homologous recombination using dsDNA repair in JM8A3 ES cells

JM8A3

Unknown

Human TRAF6 DNA consisting of CDS (exons 3-17) fused to mouse Traf6 3'UTR was inserted into exon 3 beginning at the 15th coding residue of mouse Nrp1 gene. This approach minimizes potential downstream splicing or RNP re-cleavage as the guide protospacer was split. Expression of the human CDS is primarily controlled by the mouse regulatory system and is expected to not transcribe the mouse version. CRISPR RNP was used to assist with homologous recombination using dsDNA repair in JM8A3 ES cells.

C57BL/6N

The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Kent Lloyd, D.V.M., University of California, Davis.

Not ready to publish