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Strain Detail Sheet

Published: 10/15/2025
Revised: 10/15/2025

Strain Name:
CC040.129S6-Isr(HSA10;BORCS7-AS3MT)1Bhk/PmvMmnc
Stock Number:
075852-UNC
Citation ID:
RRID:MMRRC_075852-UNC
Other Names:
CC040.129S6-hAS3MT

Strain Information

Gene Details

Isr(HSA10;BORCS7-AS3MT)1Bhk
Name: interspecies replacement, human Chr 10, Beverly H Koller 1; interspecies replacement, human Chr 10, Beverly H Koller 1
Synonyms: Tc(HSA10)1Pmv
Type: Allele
Species: Multi-species
Chromosome: 19
Alteration at locus: Insertion
As3mt
Name: arsenite methyltransferase
Synonyms: Cyt19, 2310045H08Rik
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Insertion
NCBI: 57344
VEGA: 19
Homologene: 10754

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Borcs7
Name: BLOC-1 related complex subunit 7
Synonyms: 2010012O05Rik
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Insertion
NCBI: 66439
VEGA: 19
Homologene: 16941

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
AS3MT
Name: arsenite methyltransferase
Type: Gene
Species: Homo sapiens (human)
Chromosome: 10
Alteration at locus: Insertion
BORCS7
Name: BLOC-1 related complex subunit 7
Type: Gene
Species: Homo sapiens (human)
Chromosome: 10
Alteration at locus: Insertion
Genetic Alterations
The entire As3mt locus was replaced with it's human ortholog (AS3MT) via syntenic replacement.The targeting construct replaced the 60,908 bp segment extending from chr19:46,683,032 to 46,744,011 with a 59,261-bp segment of human DNA extending from chr10:102,845,563 to 102,904,823 and a floxed neomycin resistance cassette.

Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Phnx43 derived from 129S6/SvEvTac

    Strain Description

    Phenotype

    Mice metabolize arsenic less effectively.

    Strain GQC Summary
    Gene Specific Genotyping:

    To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

    Background Genetic Quality:

    The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

    Suggested Control Mice
    Littermates of all relevant genotypes.

    Research Applications

    • Diabetes
    • Metabolism
    • Models for Human Disease
    • Obesity
    • Research Tools

    Strain Origin

    Donor
    Fernando Pardo-Manuel de Villena, Ph.D., University of North Carolina at Chapel Hill School of Medicine.
    Primary Reference
    In preparation, submitted, or in press
    Additional References

    Koller BH, Snouwaert JN, Douillet C, Jania LA, El-Masri H, Thomas DJ, Stýblo M. Arsenic Metabolism in Mice Carrying a BORCS7/AS3MT Locus Humanized by Syntenic Replacement. Environ Health Perspect. 2020 Aug;128(8):87003. doi: 10.1289/EHP6943. Epub 2020 Aug 11. (Medline PMID: 32779937)

    Strain Development
    Phnx 43 129S6/SvEvTac ES cells carrying the human BORCS7/AS3MT locus were injected into blastocysts and the blastocysts were transfered to pseudopregnant dams. To maintain the mutation on the 129S6/SvEvTac background, the resulting chimeric mice were bred to 129S6/SvEvTac mice. F1 mice were identified by Taq as wild-type (WT) homozygotes for the mouse As3mt/Borcs7 locus (WT/WT), or heterozygous (WT/Hs) or homozygous (Hs/Hs) for the human AS3MT/BORCS7 locus. The PCR assays were carried out using Taq polymerase. The F2 generation—consisting of Hs/Hs homozygotes, Hs/WT heterozygotes, and WT/WT homozygotes—were produced by mating of F1 Hs/WT heterozygotes. The mice were then bred to CC040 Collaborative Cross (CC) MMRRC: 66465 mice to generate a congenic line carrying the human AS3MT gene on a diverse genetic background.


    Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    For more information about this colony's health status contact mmrrc_health@med.unc.edu

    Appearance

    Coat Color
    Agouti
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Sib-mating
    Generation
    N/A
    Overall Breeding Performance
    Poor

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Variable Variable
    Average litter size
    Variable
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    Variable

    Order Information

    Availability Level

    Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at mmrrc@med.unc.edu for more details.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    075852-UNC-SPERM Cryo-preserved spermatozoa $520.00 / $863.00
    Non-Profit / For-Profit    		 Aliquot Approximate quantity3
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.