• Donor's Genotyping Strategies Overview
• Donor's Protocol for Genotyping by Dot Blot Analysis
• 5'RACE Sequence Tag Generation Protocol
• 5'RACE Sequencing Outline
• 5'RACE Sequencing Protocol

• MMRRC's Cell Culture Protocol
• Donor's Cell Culture Protocol
• Electroporation and Isolation of ES Cell Lines
• Blastocyst Injections and Mouse Breeding
• PLAP Staining
• Taqman 7700 QPCR Assay to Assess Neo Dosage in Knockout Mice
• Paraffin Embedding and Sectioning of Mouse Embryos
• In Situ Hybridization with DIG-probes
• ALL experimental protocols from BayGenomics

ES Cell Sex and Genotype: These ES Cells are expected to be male (XY) and heterozygous/hemizygous for the mutation.

Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. The parental cell line E14Tg2a.4, from which these cells were derived, was tested for multiple mouse pathogens; please see the Health Status Report.

Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@ucdavis.edu.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.