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Pettitt SJ, Liang Q, Rairdan XY, Moran JL, Prosser HM, Beier DR, Lloyd KC,Bradley A, Skarnes WC. Agouti C57BL/6N embryonic stem cells for mouse geneticresources. Nat Methods. 2009 Jul;6(7):493-5. doi: 10.1038/nmeth.1342. Epub 2009Jun 14. (Medline PMID: 19525957)
These cells are expected to be male (XY), are feeder-dependent, and homozygous (a/a) for the a, non-agouti, coat color allele.
Requested cell lines are tested by PCR analysis for mycoplasma spp. and Murine Parvoviruses (MPV) upon completion of expansion and prior to shipment. The JM8 Parental Cell Line, from which these cells were derived, was tested for multiple mouse pathogens and all results were negative. The specific JM8A1.N3 Parental Cell Sub-line was also tested for multiple mouse pathogens; please see the Health Status Report.
Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email firstname.lastname@example.org.
Distribution of this strain requires submission of the JM8 Sub-line MTA
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.