Loading Mouse GIF
Loading...

Strain Name:
B6.129S6-Nfiatm1Rmg/Mmnc
Stock Number:
010318-UNC
Citation ID:
RRID:MMRRC_010318-UNC

Strain Information

Nfiatm1Rmg
Name: nuclear factor I/A; targeted mutation 1, Richard M Gronostajski
Synonyms: Nfia-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Knockout
Nfia
Name: nuclear factor I/A
Synonyms: 1110047K16Rik, NF1-A, 9430022M17Rik, NF1A
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Knockout
NCBI: 18027
HGNC: HGNC:7784
Homologene: 4086
Genetic Alterations
A neo cassette was used to replace most of exon 2
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    E14 derived from 129P2/OlaHsd
    Phenotype
    Homozygous: Agenesis of the corpus callosum and hippocampal commisure. Perinatal lethality (100% in C57Bl/6, 70% in 129S6). Hydrocephalus in surviving 129S6 pups.

    Heterozygous: Some reduction in viability in mixed Black Swiss background but not in C57BL/6 strain
    Mammalian Phenotype Terms
    Allelic Composition: Nfiatm1Rmg/Nfiatm1Rmg (Genetic Background: B6.129P2-Nfiatm1Rmg )

    Allelic Composition: Nfiatm1Rmg/Nfiatm1Rmg (Genetic Background: involves: 129P2/OlaHsd * Black Swiss )

    Allelic Composition: Nfiatm1Rmg/Nfiatm1Rmg (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

    Allelic Composition: Nfiatm1Rmg/Nfiatm1Rmg (Genetic Background: involves: 129P2/OlaHsd * C57BL/6N )

    Allelic Composition: Nfiatm1Rmg/Nfia+ (Genetic Background: involves: 129P2/OlaHsd * Black Swiss )

    Allelic Composition: Nfiatm1Rmg/Nfia+ (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

    MeSH Terms
    • Animals
    • Brain/metabolism
    • CCAAT-Enhancer-Binding Proteins
    • Corpus Callosum/physiology
    • DNA-Binding Proteins/genetics
    • DNA-Binding Proteins/physiology
    • Female
    • Fertility/genetics
    • Fibroblasts/metabolism
    • Glial Fibrillary Acidic Protein/metabolism
    • Hydrocephalus/genetics
    • Male
    • Mice
    • Mice, Inbred C57BL
    • Models, Genetic
    • Mutagenesis
    • NFI Transcription Factors
    • Nuclear Proteins
    • Phenotype
    • Recombination, Genetic
    • Stem Cells/metabolism
    • Transcription Factors
    • Y-Box-Binding Protein 1
    • Agenesis of Corpus Callosum
    • CCAAT-Enhancer-Binding Proteins/genetics
    • CCAAT-Enhancer-Binding Proteins/physiology
    • Corpus Callosum/cytology
    • Corpus Callosum/embryology
    • DNA-Binding Proteins
    • Hippocampus/abnormalities
    • Hippocampus/cytology
    • Hippocampus/embryology
    • Intercellular Signaling Peptides and Proteins
    • Mice, Knockout
    • Nerve Tissue Proteins/metabolism
    • Neuroglia/cytology
    • Perforant Pathway
    • Prosencephalon/abnormalities
    • Prosencephalon/cytology
    • Prosencephalon/embryology
    • Receptors, Immunologic/metabolism
    • Telencephalon/cytology
    • Telencephalon/embryology
    • Telencephalon/metabolism
    Suggested Control Mice
    • Wildtype littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Developmental Biology
    • Models for Human Disease
    • Neurobiology
    Donor
    Richard Gronostajski, Ph.D.,  University at Buffalo
    Primary Reference
    • das Neves L, Duchala CS, Tolentino-Silva F, Haxhiu MA, Colmenares C, Macklin WB, Campbell CE, Butz KG, Gronostajski RM. FDisruption of the murine nuclear factor I-A gene (Nfia) results in perinatal lethality, hydrocephalus, and agenesis of the corpus callosum. Proc Natl Acad Sci U S A. 1999 Oct 12;96(21):11946-51. Erratum in: Proc Natl Acad Sci U S A 2001 Mar 27;98(7):4276. Godinho F [corrected to Tolentino-Silva F]. (Medline PMID: 10518556)
    • Shu T, Butz KG, Plachez C, Gronostajski RM, Richards LJ. Abnormal development of forebrain midline glia and commissural projections in Nfia knock-out mice. J Neurosci. 2003 Jan 1;23(1):203-12. (Medline PMID: 12514217)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.
    Coat Color
    Black
    MMRRC Breeding System
    Backcross
    Generation
    N20
    Overall Breeding Performance
    Good (for Hets)
    Average litter size
    6-8
    Recommended wean age
    3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    010318-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.