Strain Detail Sheet

Strain Name    :

B6.129P2-Terttm1Leah/Mmucd

Stock Number :

010432-UCD

Other Names   :

mTert-/-, B6.129P2-Terttm1Leah/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Terttm1Leah
Name: targeted mutation 1, Lea Harrington
Alteration at locus: Knockout
Gene Symbol: Tert
Name: telomerase reverse transcriptase
Chromosome: 13
Alteration at locus: Knockout

Genetic Alterations:
PGK-neo disruption of the telomerase-specific motif and the first 3 of 7 reverse transcriptase motifs of Tert

Genotype Determination:

ES Cell Line: E14 derived from 129P2

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Telomere shortening, eventual sterility and genome/telomere instability.

Heterozygous phenotype: Intermediate telomere shortening, mice remain fertile and show no evidence of overt genome/telomere instability.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Terttm1Leah/Tert+
        involves: 129P2/OlaHsd * C57BL/6J
  • cellular phenotype
    • abnormal telomere length (MGI Ref ID J:90866)
      • late passages (P25 and P50) but not early passages (P1) of heterozygous mutant ES cells show a modest reduction in telomeric restriction fragment (TRF) length by pulsed-field gel electrophoresis; not observed in early or late passages of wild-type ES cells
      • P50 but not P1 heterozygous mutant ES cells show decreased telomere signal intensity at some chromosome ends, as shown by FISH analysis on metaphase spreads
      • however, no end-to-end chromosomal fusions are detected in P50 heterozygous mutant ES cells, despite the presence of shortened telomeres
    • aneuploidy (MGI Ref ID J:90866)
      • late passages of heterozygous mutant ES cells display increased aneuploidy relative to wild-type ES cells
Terttm1Leah/Terttm1Leah
        involves: 129P2/OlaHsd * C57BL/6J
  • cellular phenotype
    • abnormal telomere length (MGI Ref ID J:90866)
      • although homozygotes are viable and overtly normal for at least 4 generations and splenocytes and thymocytes derived from first generation (G1) homozygotes display a similar telomere signal intensity as wild-type G1 littermates by flow-FISH analysis, cells from G2 homozygotes show a significant reduction in telomere length relative to wild-type and G1 homozygotes
      • in addition, late passages (P25 and P50) but not early passages (P1) of homozygous mutant ES cells show a modest reduction in telomeric restriction fragment (TRF) length by pulsed-field gel electrophoresis; not observed in early or late passages of wild-type ES cells
    • aneuploidy (MGI Ref ID J:90866)
      • late passages of homozygous mutant ES cells display increased aneuploidy relative to wild-type ES cells
    • chromosomal instability (MGI Ref ID J:90866)
      • late passages (P50) of homozygous mutant ES cells show decreased telomere signal intensity at some chromosome ends, as shown by FISH analysis on metaphase spreads
      • only late passages (P50) of homozygous mutant ES cells (~300 population doublings) show a significant increase in end-to-end chromosome fusions
      • however, mitogen-stimulated cells from spleen of G1 and G2 homozygous mutant mice do not display any increase in chromosomal aberrations relative to wild-type littermates, indicating that end-to-end fusions appear only in later generations

Strain of Origin: mixed, 129J and C57BL/6

Strain genetic background: C57BL/6

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cancer
  • Models for Human Disease

Strain Origin

Donor: Lea Harrington, Ph.D., Ontario Cancer Institute

Primary Reference:

  • Liu Y, Snow BE, Hande MP, Yeung D, Erdmann NJ, Wakeham A, Itie A, Siderovski DP, Lansdorp PM, Robinson MO, Harrington L. The telomerase reverse transcriptase is limiting and necessary for telomerase function in vivo. Curr Biol. 2000 Nov 16;10(22):1459-62. Erratum in: Curr Biol 2001 Jun 5;11(11):907. (Medline PMID: 11102810)
  • Liu Y, Kha H, Ungrin M, Robinson MO, Harrington L. Preferential maintenance of critically short telomeres in mammalian cells heterozygous for mTert. Proc Natl Acad Sci U S A. 2002 Mar 19;99(6):3597-602. (Medline PMID: 11904422)
  • Erdmann N, Liu Y, Harrington L. Distinct dosage requirements for the maintenance of long and short telomeres in mTert heterozygous mice. Proc Natl Acad Sci U S A. 2004 Apr 20;101(16):6080-5. Epub 2004 Apr 12. (Medline PMID: 15079066)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
010432-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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