Strain Detail Sheet

Strain Name    :

B6;129-Erbb4tm1Grl/Mmucd

Stock Number :

010461-UCD

Other Names   :

ErbB4 +/-, B6;129-Erbb4tm1Grl/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Erbb4tm1Grl
Name: targeted mutation 1, Greg Lemke
Alteration at locus: Knockout
Gene Symbol: Erbb4
Name: v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
Chromosome: 1
Alteration at locus: Knockout

Genetic Alterations:
Isolated genomic clones of the Erbb4 gene from a 129/Sv genomic library were used to prepare a pPNT based targeting vector. This construct was electroporated into R1 ES cells, microinjected into C57BL/6J blastocysts, and these were transferred to pseudo-pregnant recipient females to generate chimeric mice.

Genotype Determination:

ES Cell Line: R1 derived from 129

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous knockout is embryonic lethal at 10.5dpc due to aborted development of the myocardial trabeculae of the heart ventricle.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Erbb4tm1Grl/Erbb4+
        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • behavior/neurological phenotype
    • hyperactivity (MGI Ref ID J:81106)
      • animals are significantly more active than controls in an open field test, but less significantly than Nrg1 heterozygotes
  • nervous system phenotype
    • abnormal prepulse inhibition (MGI Ref ID J:81106)
      • impairment was seen, but not as severely as in the Nrg1 heterozygous mice
      • normal acoustic startle response was observed
Erbb4tm1Grl/Erbb4tm1Grl
        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • mortality/aging
    • complete embryonic lethality during organogenesis (MGI Ref ID J:29972)
      • homozygous embryos die between E10 and E11
  • cardiovascular system phenotype
    • abnormal blood flow velocity (MGI Ref ID J:29972)
      • severely reduced embryonic blood flow as evidenced by an increased number of red blood cells in the heart
    • absent trabeculae carneae (MGI Ref ID J:29972)
    • decreased atrioventricular cushion size (MGI Ref ID J:29972)
      • the remainder of the endocardium appears normal
  • nervous system phenotype
    • abnormal cranial ganglia morphology (MGI Ref ID J:29972)
      • abnormal geniculate ganglion morphology (MGI Ref ID J:29972)
        • nearly fused to ganglion V (trigeminal)
      • abnormal trigeminal ganglion morphology (MGI Ref ID J:29972)
        • nearly fused to ganglion VII/VIII (geniculate/vestibulocohlear)
    • abnormal cranial nerve morphology (MGI Ref ID J:29972)
      • abnormal facial nerve morphology (MGI Ref ID J:29972)
        • abnormal neuronal projections to the hindbrain
      • abnormal glossopharyngeal nerve morphology (MGI Ref ID J:29972)
        • mispositioned caudally
      • abnormal trigeminal nerve morphology (MGI Ref ID J:29972)
        • abnormal neuronal projections to the hindbrain
      • abnormal vestibulocochlear nerve morphology (MGI Ref ID J:29972)
    • abnormal motor neuron innervation (MGI Ref ID J:29972)
      • the geniculate (facial) ganglion exhibits motor axon projections to rhombomeres 1, 2, 3, 4 and 5 instead of just 4 and 5 as seen in controls
    • abnormal sensory neuron innervation pattern (MGI Ref ID J:29972)
      • abnormal innervation to and from the hindbrain to the crainial ganglia observed at E10.5
      • the geniculate (facial) ganglion exhibits sensory neuron projections to rhombomeres 2 and 4 compared to only rhombomere 2 in controls
      • the trigeminal ganglion exhibits sensory neuron projections to rhombomere 2 and 4 compared to only rhombomere 2 in controls
    • abnormal vestibulocochlear ganglion morphology (MGI Ref ID J:29972)
      • nearly fused to ganglion V (trigeminal)
  • muscle phenotype
    • absent trabeculae carneae (MGI Ref ID J:29972)

Strain of Origin: 129

Strain genetic background: C57BL/6

Strain Development: Chimeric males were mated to C57BL/6J female to determine germline transmission. Subsequent heterozygous siblings were then bred, strain has been maintained by sibling mating.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cardiovascular
  • Developmental Biology
  • Internal/Organ
  • Models for Human Disease
  • Neurobiology
  • Research Tools

Strain Origin

Donor: Greg Lemke, Ph.D., The Salk Institute

Primary Reference:

Gassmann M, Casagranda F, Orioli D, Simon H, Lai C, Klein R, Lemke G. Aberrant neural and cardiac development in mice lacking the ErbB4 neuregulin receptor. Nature. 1995 Nov 23;378(6555):390-4. (Medline PMID: 7477376)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Backcross

Breeding Scheme(s):

  • C57BL/6J female x Heterozygous male
  • Heterozygous female x C57BL/6J male

Generation: N3 As of: 7/26/05

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 32 weeks 36 weeks

Average litter size: 6

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
010461-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
010461-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
010461-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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