Strain Name    : C57BL/6J-Myd88poc/Mmucd

Stock Number : 010475-UCD

Other Names   : Pococurante, C57BL/6J-Myd88poc/Mmcd

Gene Information

(provided by MGI)
Allele Symbol: Myd88poc
Name: pococurante
Chromosome: 9
Alteration at locus: Chemically Induced
Gene Symbol: Myd88
Name: myeloid differentiation primary response gene 88
Chromosome: 9
Alteration at locus: Chemically Induced
Genetic Alterations:
MyD88-I179N

Genotype Determination:

ES Cell Line: Not applicable

Additional mutations found on this strain:

AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01340:Myd88 APN 9 119337352 splice site 0.00
lackadaisical UTSW 9 119338692 missense probably damaging 1.00
Myd88rev1 UTSW 9 119337394 missense probably damaging 0.99
R1695:Myd88 UTSW 9 119337842 nonsense probably null
R1878:Myd88 UTSW 9 119338620 missense probably benign 0.00
R2413:Myd88 UTSW 9 119337418 missense probably benign 0.12
R3417:Myd88 UTSW 9 119337490 missense possibly damaging 0.90
R3836:Myd88 UTSW 9 119338193 splice acceptor site
R3892:Myd88 UTSW 9 119337816 missense possibly damaging 0.93
R4081:Myd88 UTSW 9 119339987 missense no information

Phenotype:

Homozygous phenotype: Similar to Myd88 knockout, but show normal response to MALP2 and PAM2.

Heterozygous phenotype: Normal as wild type

Mammalian Phenotype Terms:
Allelic Composition: Myd88poc/Myd88poc (Genetic Background: C57BL/6-Myd88poc )

Allelic Composition: Myd88poc/Myd88+ (Genetic Background: C57BL/6-Myd88poc )

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: Homozygous mutant was picked up by the screening, and backcrossed to its parents, after that homozygotes were kept by sib-mating.

Suggested Control Mice: Wild-type littermates

  • Immunology and Inflammation
  • Virology

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

  • Jiang Z; Georgel P; Li C; Choe J; Crozat K; Rutschmann S; Du X; Bigby T; Mudd S; Sovath S; Wilson IA; Olson A; Beutler B, Details of Toll-like receptor:adapter interaction revealed by germ-line mutagenesis., Proc Natl Acad Sci U S A 2006 Jul 18;103(29):10961-6 (Medline PMID: 16832055)
  • Croker BA; Lawson BR; Berger M; Eidenschenk C; Blasius AL; Moresco EM; Sovath S; Cengia L; Shultz LD; Theofilopoulos AN; Pettersson S; Beutler BA, Inflammation and autoimmunity caused by a SHP1 mutation depend on IL-1, MyD88, and a microbial trigger., Proc Natl Acad Sci U S A 2008 Sep 30;105(39):15028-33 (Medline PMID: 18806225)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
010475-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
010475-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
010475-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.