Strain Name:
C57BL/6-Hcfc2fkls/Mmucd
Stock Number:
010477-UCD
Citation ID:
RRID:MMRRC_010477-UCD
Other Names:
Feckless, Feckless/Mmcd
Major Collection:

Strain Information

Hcfc2fkls
Name: host cell factor C2; feckless
Synonyms: Hcfc2fls
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
Hcfc2
Name: host cell factor C2
Synonyms: fkls, 1700129L13Rik
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 67933
Homologene: 8337
Genetic Alterations
Per MGI:5445585, allele is Hcfc2fkls
  • gene is Hcfc2 (MGI:1915183)
  • RefSeq (other isoforms known): NM_001081218.1
  • MGI reports the genetic alteration as "ENU-mutagenis induced a C to T nucleotide substitution that results in the amino acid substitution of arginine for tryptophan at position 296 (W296R). (J:251008)"; 
    Sequence analysis of the NM_001081218.1 confirmed a Trp at codon 296. However, tryptophan is encoded only by TGG; there is no other codon that would involve a cytosine. The purported C>T exchange cannot be true. Likely, the reverse is true, i.e., T>C as that would afford an easy explanation with c.886T>C, p.Trp296Arg. 
Genotype Determination
Donor protocol, please see here.
Phenotype
Homozygous: No response to viral dsRNA

Heterozygous: Same as wild type
Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

Strain Development
Homozygous mutant was picked up by screening, and backcrossed to its parents, after that homozygotes were kept by sib-mating.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Immunology and Inflammation
  • Virology
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Bred to Homozygosity
No

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
010477-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
010477-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
010477-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.