Strain Detail Sheet

Strain Name    :

B6;129-Btrctm1Paga/Mmnc

Stock Number :

010480-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Btrctm1Paga
Name: targeted mutation 1, Michele Pagano
Alteration at locus: Knockout
Gene Symbol: Btrc
Name: beta-transducin repeat containing protein
Chromosome: 19
Alteration at locus: Knockout

Genetic Alterations:
A neo cassette was inserted in exon 5.

Genotype Determination:

ES Cell Line: D3 derived from 129

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Defective spermatogenesis. Defective mammary gland development in the females

Heterozygous phenotype: No defects


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Btrctm1Paga/Btrctm1Paga
        involves: 129S2/SvPas
  • mortality/aging
    • mortality/aging (MGI Ref ID J:93010)
      • homozygous mice appeared generally as healthy as wildtype littermates over 2 years' observation
      • almost all mutants revealed no gross tissue abnormalities upon necropsy
      • exceptions were a single invasive intestinal carcinoma in a 10 month-old mouse and death due to thymic lymphoma of 2 mice aged 6.5 and 9 months
  • cellular phenotype
    • abnormal mitosis (MGI Ref ID J:93010)
      • cultured homozygous mouse embryonic fibroblasts (MEFs) were retarded in their progression through mitosis, despite normal passage from G1 to S phase
      • MEFs exhibited misalignment of chromosomes at the metaphase plate
      • approximately 20% of asynchronously dividing MEFs were found to have supernumerary centrosomes, and approximately 12 % of dividing MEFs exhibited multipolar spindles
      • abnormal mitotic spindle (MGI Ref ID J:93010)
        • approximately 12 % of dividing MEFs exhibited multipolar spindles
  • reproductive system phenotype
    • abnormal male meiosis (MGI Ref ID J:93010)
      • histological examination of epidydimes of homozygous males revealed evidence of prolonged, abnormal meiosis
      • numbers of metaphase I spermatocytes were elevated
      • in some mice, spindle abnormalities and misaligned chromosomes were observed
      • the degree of fertility impairment correlated with the severity of histologic abnormality
    • abnormal meiotic spindle (MGI Ref ID J:93010)
      • in some spermatocytes, spindle abnormalities and misaligned chromosomes were observed
    • oligozoospermia (MGI Ref ID J:93010)
      • histological examination of epidydimes of homozygous males revealed severely diminished numbers of mature spermatozoa
    • reduced male fertility (MGI Ref ID J:93010)
      • approximately 50% of homozygous male mice produced no progeny when paired with wild-type females
      • fertility defect was not behavioral in origin, as mating behavior was normal and vaginal plugs were observed
      • productive homozygous males sired fewer and smaller litters, on average, than did wild-type males

Strain of Origin: 129

Strain genetic background: C57BL/6

Strain Development: The original C57BL/6 X 129 male chimeras were mated with heterozygous wild type C57BL/6 females. The heterozygous were then intercrossed to obtain the F2 generation. Following generations were obtained by intercrossing heterozygous mice.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6;129-Btrctm1Paga/Mmnc MMRRC:010480-UNC C57BL/6J 129S1/SvImJ 1717 56 809 0


Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cancer
  • Cell Biology

Strain Origin

Donor: Michele Pagano, Ph.D., New York University School of Medicine

Primary Reference:

  • Guardavaccaro D, Kudo Y, Boulaire J, Barchi M, Busino L, Donzelli M, Margottin-Goguet F, Jackson PK, Yamasaki L, Pagano M. Control of meiotic and mitotic progression by the F box protein beta-Trcp1 in vivo. Dev Cell. 2003 Jun;4(6):799-812. (Medline PMID: 12791266)
  • Kudo Y, Guardavaccaro D, Santamaria PG, Koyama-Nasu R, Latres E, Bronson R, Yamasaki L, Pagano M. Role of F-box protein betaTrcp1 in mammary gland development and tumorigenesis. Mol Cell Biol. 2004 Sep;24(18):8184-94. (Medline PMID: 15340078)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
010480-UNC-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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