Strain Name    : C.129-Thy1a Foxp3sf/Mmnc

Stock Number : 010500-UNC

Gene Information

(provided by MGI)
Allele Symbol: Thy1a
Name: a variant
Chromosome: 9
Alteration at locus: Spontaneous Mutation
Allele Symbol: Foxp3sf
Name: scurfy
Chromosome: X
Alteration at locus: Spontaneous Mutation
Gene Symbol: Thy1
Name: thymus cell antigen 1, theta
Chromosome: 9
Alteration at locus: Spontaneous Mutation
Gene Symbol: Foxp3
Name: forkhead box P3
Chromosome: X
Alteration at locus: Spontaneous Mutation
Genetic Alterations:
Scurfy is a spontaneous mutation in the foxp3 gene on the X-chromosome.

Genotype Determination:

  • The genotyping protocol for this strain is currently not available. The MMRRC will develop a protocol if this strain is ordered.

ES Cell Line:

Phenotype

Homozygous phenotype: Not applicable

Hemizygous phenotype: Hemizygous males die at 25-35 days of age with immune dysregulation and cytokine toxicity. Scurfy males lack CD4+CD25+ regulatory T cells. Clinical signs include enlarged lymph nodes, hepatosplenomegaly, scaly skin, anemia, diarrhea.

Mammalian Phenotype Terms:

Strain of Origin: 129/Rl (ORNL)

Strain genetic background: BALB/c-Thy1.1

Strain Development: 129/Rl-sf N105 obtained from ORNL in 2000. BALB/c-Thy1.1 obtained from Shimon Sakaguchi (Japan) and congenic strain was created by 10 backcrosses to this BALB/c substrain.

Suggested Control Mice:

  • Wildtype littermates

  • Immunology and Inflammation
  • Models for Human Disease

Donor: Virginia Godfrey, DVM, Ph.D., University of North Carolina

Primary Reference:

  • Ramsdell F. Foxp3 and natural regulatory T cells: key to a cell lineage? Immunity. 2003 Aug;19(2):165-8. Review. (Medline PMID: 12932350)
  • Fontenot JD, Gavin MA, Rudensky AY. Foxp3 programs the development and function of CD4+CD25+ regulatory T cells. Nat Immunol. 2003 Apr;4(4):330-6. Epub 2003 Mar 3. (Medline PMID: 12612578)

Colony and Husbandry Information

Scurfy males are very susceptible to colitis induced by Helicobacter infection. This strain is best maintained in sterilized microisolator conditions that prevent the introduction of Helicobacter sp.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
010500-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
010500-UNC-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.