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Strain Name:
C.129-Thy1a Foxp3sf/Mmnc
Stock Number:
010500-UNC
Citation ID:
RRID:MMRRC_010500-UNC

Strain Information

Thy1a
Name: thymus cell antigen 1, theta; a variant
Synonyms: thetaAKR, theta-AKR, Thy1.1, Thy-1.1, Thy1a
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Spontaneous Mutation
Foxp3sf
Name: forkhead box P3; scurfy
Synonyms: Scurfy, sf
Type: Allele
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Spontaneous Mutation
Thy1
Name: thymus cell antigen 1, theta
Synonyms: CD90, Thy-1, Thy 1.2, Thy1.1, Thy-1.2, Thy1.2, T25
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Spontaneous Mutation
NCBI: 21838
Homologene: 4580
Foxp3
Name: forkhead box P3
Synonyms: scurfin, JM2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Spontaneous Mutation
NCBI: 20371
HGNC: HGNC:6106
Homologene: 8516
Genetic Alterations
Scurfy is a spontaneous mutation in the foxp3 gene on the X-chromosome.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Homozygous: Not characterized.

    Hemizygous: Hemizygous males die at 25-35 days of age with immune dysregulation and cytokine toxicity. Scurfy males lack CD4+CD25+ regulatory T cells. Clinical signs include enlarged lymph nodes, hepatosplenomegaly, scaly skin, anemia, diarrhea.
    MeSH Terms
    • Animals
    • Cell Differentiation
    • DNA-Binding Proteins/genetics
    • DNA-Binding Proteins/metabolism
    • Forkhead Transcription Factors
    • Immune Tolerance
    • Mice
    • Models, Immunological
    • Mutation
    • T-Lymphocyte Subsets/cytology
    • T-Lymphocyte Subsets/immunology
    • T-Lymphocyte Subsets/metabolism
    • T-Lymphocytes, Regulatory/cytology
    • T-Lymphocytes, Regulatory/immunology
    • T-Lymphocytes, Regulatory/metabolism
    • CD4-Positive T-Lymphocytes/immunology
    • CD4-Positive T-Lymphocytes/metabolism
    • DNA-Binding Proteins/immunology
    • Down-Regulation
    • Female
    • Gene Targeting
    • Receptors, Interleukin-2/immunology
    • Receptors, Interleukin-2/metabolism
    Strain Development
    129/Rl-sf N105 obtained from ORNL in 2000. BALB/c-Thy1.1 obtained from Shimon Sakaguchi (Japan) and congenic strain was created by 10 backcrosses to this BALB/c substrain.
    Suggested Control Mice
    • Wildtype littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Immunology and Inflammation
    • Models for Human Disease
    Donor
    Virginia Godfrey, DVM, Ph.D., University of North Carolina at Chapel Hill School of Medicine
    Primary Reference
    • Ramsdell F. Foxp3 and natural regulatory T cells: key to a cell lineage? Immunity. 2003 Aug;19(2):165-8. Review. (Medline PMID: 12932350)
    • Fontenot JD, Gavin MA, Rudensky AY. Foxp3 programs the development and function of CD4+CD25+ regulatory T cells. Nat Immunol. 2003 Apr;4(4):330-6. Epub 2003 Mar 3. (Medline PMID: 12612578)

    Colony and Husbandry Information

    Scurfy males are very susceptible to colitis induced by Helicobacter infection. This strain is best maintained in sterilized microisolator conditions that prevent the introduction of Helicobacter sp.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    010500-UNC-RESUS Litter recovered from cryo-archive $3,088.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.