Strain Name:
B6;129P2-Ing4Gt(XG370)Byg/Mmucd
Stock Number:
010564-UCD
Citation ID:
RRID:MMRRC_010564-UCD
Other Names:
XG370, B6;129P2-Ing4Gt(XG370)Byg/Mmcd
Major Collection:

Strain Information

Ing4Gt(XG370)Byg
Name: inhibitor of growth family, member 4; gene trap XG370, BayGenomics
Synonyms: Ing4Gt(pGT1Lxf)1Ucd
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Gene Trap
Ing4
Name: inhibitor of growth family, member 4
Synonyms: D6Xrf92, p29ING4, D6Wsu147e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Gene Trap
NCBI: 28019
Homologene: 22952
Genetic Alterations

This sequence was obtained by 5'RACE PCR from a 129P2/OlaHsd mouse embryonic stem (ES) cell line (E14TG2a.4) with an insertional mutation from gene trap vector pGT1Lxf ,   (Properly spliced).

BayGenomics and SIGTR gene trap ES cell lines have a 5 prime sequence tag, therefore you are dealing with a conventional gene trap. This means your gene trap vector is located in the intron immediately downstream transcriptionally of the last exon blast hit. Vector insertions near the 5' end of a gene are more likely to result in complete gene inactivation than insertion at the 3' end, because a greater percentage of a gene's exons are prevented from being transcribed and translated. Not all genetrap cell lines provide 100% gene knock out. Please see additional information about Gene Trap lines on our ES cell lines information page.

Genotype Determination

This mouse line was confirmed via generic b-Geo screening; we do not have any information regarding the exact genomic insertion site of the vector within the intron. We recommend blasting the sequence tag to estimate the location of a gene trap. The vast majority of gene trap lines have been correctly identified, as judged by DNA sequencing (i.e. repeat determination of the DNA sequence tag when the ES cell line is requested). However, incorrectly identified gene trap cell lines are occasionally encountered, unless further verification was done, such as 5 RACE (Rapid Amplification of cDNA Ends), to check if the sequence of the cell line matches that which is published by the donating institution. We recommend that recipients of gene trap mouse lines re-verify the identity of the ES cell line by RT-PCR and/or Southern blot analysis. To determine the exact genomic insertion site of the vector within the intron requires Vector Integration Site Analysis (VISA); please contact mmrrc@ucdavis.edu for more information on VISA services.

ES Cell Line
E14TG2a.4 derived from 129P2/OlaHsd
Phenotype
These mice were not phenotyped prior to cryopreservation, nor were they mated to produce homozygous offspring, therefore the homozygous phenotype is unknown.
Mammalian Phenotype Terms
Allelic Composition: Ing4Gt(XG370)Byg/Ing4Gt(XG370)Byg (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

MeSH Terms
  • Animals
  • Cell Line
  • Databases, Nucleic Acid
  • Embryo, Mammalian/cytology
  • Genomics
  • Internet
  • Mice/genetics
  • Mice, Knockout
  • Mutagenesis, Insertional
  • Mutation
  • Stem Cells/cytology
  • User-Computer Interface
Strain Development
The ES cell line was injected into C57BL/6J host blastocysts to generate chimeras.  The chimeras were then crossed with C57BL/6J mice to determine germline transmission.  The resulting heterozygous males were cryoarchived for distribution.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Cell Biology
  • Research Tools
Donor
MMRRC at University of California, Davis
Primary Reference
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE. BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. Nucleic Acids Res. 2003 Jan 1;31(1):278-81. (Medline PMID: 12520002)

Colony and Husbandry Information

*During development at MMRRC at UCD, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the background strain.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Agouti/Black
(Note: if sib-mated, it is possible to get pink-eyed chinchilla, the color of the 129P2/Ola, or other mixed variations.)
MMRRC Breeding System
Backcross or Inbreeding
Breeding Scheme(s)
  • C57BL/6J female x Heterozygous male or reciprocal
  • Wild-type female x Heterozygous male sibling or reciprocal
Generation
N2
Overall Breeding Performance
Good
Average litter size
8
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
010564-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
010564-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.