Strain Name:
B6.129S1-Uchl3tm1Tilg/Mmmh
Stock Number:
010587-MU
Citation ID:
RRID:MMRRC_010587-MU
Other Names:
ubiquitin C-terminal hydrolase L3

Strain Information

Uchl3tm1Tilg
Name: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); targeted mutation 1, Shirley M Tilghman
Synonyms: Uch-L3delta3-7
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Knockout
Uchl3
Name: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Type: Gene
Species: Mouse
Chromosome: 14
Alteration at locus: Knockout
NCBI: 50933
VEGA: 14
Homologene: 4377
Genetic Alterations
Exons 3 through 7 were deleted using a targeting deletion construct containing the neomycin resistance marker.
Genotype Determination
ES Cell Line
CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Phenotype
Homozygous: Homozygous mice breed well. They have no overt developmental abnormalities (Kurihara et al., 2000). However, donor submitted a manuscript that has been accepted for publication in "Hippocampus" demonstrating that UchL3 knockout mice exhibit working memory impairments.

Heterozygous: Heterozygous mice have no overt phenotype.
Mammalian Phenotype Terms
Allelic Composition: Uchl3tm1Tilg/Uchl3tm1Tilg (Genetic Background: involves: 129S1/Sv * C57BL/6 )

Allelic Composition: Uchl3tm1Tilg/Uchl3tm1Tilg (Genetic Background: involves: 129S1/Sv * C57BL/6J )

MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Differentiation
  • Cloning, Molecular
  • Gene Expression Regulation, Developmental
  • Gene Targeting
  • Gestational Age
  • Homozygote
  • In Situ Hybridization
  • Major Histocompatibility Complex/genetics
  • Major Histocompatibility Complex/immunology
  • Mice
  • Mice, Knockout
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • RNA, Messenger/metabolism
  • Sequence Alignment
  • T-Lymphocytes/metabolism
  • Thiolester Hydrolases/genetics
  • Thiolester Hydrolases/metabolism
  • Ubiquitin Thiolesterase
  • Blotting, Northern
  • Central Nervous System/metabolism
  • Central Nervous System/pathology
  • Deglutition Disorders/genetics
  • Deglutition Disorders/pathology
  • Female
  • Gene Deletion
  • Gene Expression
  • Genotype
  • Male
  • Medulla Oblongata/metabolism
  • Medulla Oblongata/pathology
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Inbred Strains
  • Mice, Mutant Strains
  • Neurodegenerative Diseases/genetics
  • Neurodegenerative Diseases/pathology
  • Paralysis/genetics
  • Paralysis/pathology
  • RNA, Messenger/genetics
  • Tissue Distribution
  • Conditioning (Psychology)/physiology
  • Fear/physiology
  • Hippocampus/enzymology
  • Long-Term Potentiation/physiology
  • Maze Learning/physiology
  • Memory, Short-Term/physiology
  • Space Perception/physiology
  • Synaptic Transmission/physiology
  • Ubiquitin Thiolesterase/genetics
  • Ubiquitin Thiolesterase/metabolism
Strain Development
The desicription of this strain's development is described in: Kurihara LJ, Semenova E, Levorse JM, Tilghman SM. Expression and functional analysis of Uch-L3 during mouse development. Mol Cell Biol. 2000 Apr;20(7):2498-504. (Medline PMID: 10713173)
Dr. Abel's laboratory maintained these mice on C57BL/6 for at least ten generations.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Models for Human Disease
  • Neurobiology
  • Learning and Memory
Donor
Shirley Tilghman, Ph.D., Princeton University.
Ted Abel, University of Pennsylvania School of Medicine.
Primary Reference
  • Kurihara LJ, Semenova E, Levorse JM, Tilghman SM. Expression and functional analysis of Uch-L3 during mouse development. Mol Cell Biol. 2000 Apr;20(7):2498-504. (Medline PMID: 10713173)
  • Kurihara LJ, Kikuchi T, Wada K, Tilghman SM. Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia. Hum Mol Genet. 2001 Sep 1;10(18):1963-70. (Medline PMID: 11555633)
  • MA Wood, MP Kaplan, CM Brensinger, W Guo, and T Abel. Ubiquitin C-terminal hydrolase L3 (UchL3) is involved in working memory. Hippocampus. 2005;15(5):610-21.(Medline PMID: 15884048)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
010587-MU-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
010587-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
010587-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.