Strain Name    : B6.129S1-Uchl3tm1Tilg/Mmmh

Stock Number : 010587-MU

Gene Information

(provided by MGI)
Allele Symbol: Uchl3tm1Tilg
Name: targeted mutation 1, Shirley M Tilghman
Chromosome: 14
Alteration at locus: Knockout
Gene Symbol: Uchl3
Name: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Chromosome: 14
Alteration at locus: Knockout
Genetic Alterations:
Exons 3 through 7 were deleted using a targeting deletion construct containing the neomycin resistance marker.

Genotype Determination:

ES Cell Line: CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+

Phenotype:

Homozygous phenotype: Homozygous mice breed well. They have no overt developmental abnormalities (Kurihara et al., 2000). However, donor submitted a manuscript that has been accepted for publication in "Hippocampus" demonstrating that UchL3 knockout mice exhibit working memory impairments.

Heterozygous phenotype: Heterozygous mice have no overt phenotype.

Mammalian Phenotype Terms:
Allelic Composition: Uchl3tm1Tilg/Uchl3tm1Tilg (Genetic Background: involves: 129S1/Sv * C57BL/6 )

Allelic Composition: Uchl3tm1Tilg/Uchl3tm1Tilg (Genetic Background: involves: 129S1/Sv * C57BL/6J )

Strain of Origin: C57BL/6

Strain genetic background: C57BL/6

Strain Development: The desicription of this strain's development is described in: Kurihara LJ, Semenova E, Levorse JM, Tilghman SM. Expression and functional analysis of Uch-L3 during mouse development. Mol Cell Biol. 2000 Apr;20(7):2498-504. (Medline PMID: 10713173)
Dr. Abel's laboratory maintained these mice on C57BL/6 for at least ten generations.

Suggested Control Mice:

  • Wildtype littermates

  • Models for Human Disease
  • Neurobiology
  • Learning and Memory

Donor:

Primary Reference:

  • Kurihara LJ, Semenova E, Levorse JM, Tilghman SM. Expression and functional analysis of Uch-L3 during mouse development. Mol Cell Biol. 2000 Apr;20(7):2498-504. (Medline PMID: 10713173)
  • Kurihara LJ, Kikuchi T, Wada K, Tilghman SM. Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia. Hum Mol Genet. 2001 Sep 1;10(18):1963-70. (Medline PMID: 11555633 )
  • MA Wood, MP Kaplan, CM Brensinger, W Guo, and T Abel. Ubiquitin C-terminal hydrolase L3 (UchL3) is involved in working memory. Hippocampus. IN PRESS.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
010587-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.