Allele Symbol: Uchl3tm1Tilg
Name: targeted mutation 1, Shirley M Tilghman
Alteration at locus: Knockout
Gene Symbol: Uchl3
Name: ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Alteration at locus: Knockout
ES Cell Line: CJ7 derived from 129S1/Sv-Oca2+ Tyr+ Kitl+
Homozygous phenotype: Homozygous mice breed well. They have no overt developmental abnormalities (Kurihara et al., 2000). However, donor submitted a manuscript that has been accepted for publication in "Hippocampus" demonstrating that UchL3 knockout mice exhibit working memory impairments.
Heterozygous phenotype: Heterozygous mice have no overt phenotype.
Strain of Origin: C57BL/6
Strain genetic background: C57BL/6
Strain Development: The desicription of this strain's development is described in: Kurihara LJ, Semenova E, Levorse JM, Tilghman SM. Expression and functional analysis of Uch-L3 during mouse development. Mol Cell Biol. 2000 Apr;20(7):2498-504. (Medline PMID: 10713173)
Dr. Abel's laboratory maintained these mice on C57BL/6 for at least ten generations.
Suggested Control Mice:
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.