Strain Detail Sheet

Strain Name    :

C57BL/6.Cg-Tg(Tagln-cre)1Jomm/Mmnc

Stock Number :

010588-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(Tagln-cre)1Jomm
Name: transgene insertion 1, Joseph M Miano
Alteration at locus: Transgenic (cre)
Transgene: cre (Bacteriophage P1)
Name: cre recombinase
Alteration at locus: Transgenic (cre)
Promoter: Tagln
Name: transgelin
Alteration at locus: Transgenic (cre)

Genetic Alterations:
A 505 base pair promoter fragment of the SM22 gene (symbol TAGLN) was cloned upstream of a constitutively nuclear cyclization recombination gene and the linearized plasmid microinjected into oocytes for founder generation.

Genotype Determination:

  • Donor's Protocol
  • Center protocol and contact for technical support will be shipped with mice.

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Donor always maintained this transgenic line in a hemizygous state.

Hemizygous phenotype: No overt phenotype has been seen in over 10 generations.

Strain of Origin: CD-1

Strain genetic background: C57BL/6J

Strain Development: CD-1 transgenic founders were mated for 3 generations to the CD-1 strain before backcrossing at least 10 generations to the current C57BL/6 strain. Donor has consistently used Jackson Lab's stock of C57BL/6 mice for the backcross.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Models for Human Disease
  • Research Tools

Strain Origin

Donor: Joseph M Miano, Ph.D., University of Rochester School of Medicine

Primary Reference:

  • Li L, Miano JM, Mercer B, Olson EN. Expression of the SM22alpha promoter in transgenic mice provides evidence for distinct transcriptional regulatory programs in vascular and visceral smooth muscle cells. J Cell Biol. 1996 Mar;132(5):849-59. (Medline PMID: 8603917 )
  • Miano JM, Ramanan N, Georger MA, de Mesy Bentley KL, Emerson RL, Balza RO Jr, Xiao Q, Weiler H, Ginty DD, Misra RP. Restricted inactivation of serum response factor to the cardiovascular system. Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17132-7. Epub 2004 Nov 29. (Medline PMID: 15569937)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
010588-UNC-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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