Strain Detail Sheet

Strain Name    :

STOCK Aprttm1Jat Tg(pUR288)1Vij/Mmmh

Stock Number :

010589-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Aprttm1Jat
Name: targeted mutation 1, Jay A Tischfield
Alteration at locus: Knockout
Gene Symbol: Aprt
Name: adenine phosphoribosyl transferase
Chromosome: 8
Alteration at locus: Knockout
Transgene: Tg(pUR288)1Vij
Name: transgene insertion 1, Jan Vijg
Alteration at locus: Transgenic (BAC)
Transgene: pUR288 (Bacteria)
Name:
Alteration at locus: Transgenic (BAC)

Genetic Alterations:
A positive-neagative gene targeting vector, MBSF18, was constructed from 5.6 kb of mouse genomic DNA (strain 129?Sv) containing the entire coding sequence of Aprt. To verify the cloning Southern blotting analysis was performed. Chimeric mice were produced by blastocyst injection using one D3 ES cell clone, MBSF1837. Chimeric mice were mated to Black Swiss. Offspring generated was tested for heterozygozity by Southern Blot.

LacZ Mice-200 copies of the pUR288 plasmid linearized with Pst I were microinjected into fertilized C57BL/6J mouse oocytes. The offspring were analyzed for the presence of the transgene by Southern blot and bred to Homozygosity.

Genotype Determination:

ES Cell Line: Not specified derived from C57BL/6J x Black Swiss

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

The pUR288-lacZ transgenic mouse is used to detect of a broad range of mutations, varying from point mutations in the lacZ gene to large genome rearrangements. However, this system cannot detect mutations involving gain or loss of entire lacZ copies. To detect loss of individual copies of the lacZ gene, the most suitable model is the heterozygous Aprt knockout mouse model (Engle et al., 1996), which can be applied to all tissues from which cells can be grown in short-term culture. This present Aprt/lacZ tool mouse model is a hybrid of the pUR288-lacZ and the Aprt knockout mouse. The model will allow the analysis of virtually all mutational events in a broad spectrum of tissues.

Strain of Origin: Mixture of C57BL/6J and Black Swiss.

Strain genetic background: Mixture of C57BL/6J and Black Swiss.

Strain Development: LacZ animals harboring the lacZ gene chromossomally integrated head-to-tail on chromosome 11 was crossed with heterozygous animals for the Aprt allele. This cross was repeated 5 times until F5 generation was obtained.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Models for Human Disease
  • Research Tools

Strain Origin

Donor: Jan Vijg, Ph.D., Beth Israel Deaconess Medical Center

Primary Reference:

  • Engle SJ, Stockelman MG, Chen J, Boivin G, Yum MN, Davies PM, Ying MY, Sahota A, Simmonds HA, Stambrook PJ, Tischfield JA. Adenine phosphoribosyltransferase-deficient mice develop 2,8-dihydroxyadenine nephrolithiasis. Proc Natl Acad Sci U S A. 1996 May 28;93(11):5307-12. (Medline PMID: 8643571)
  • Gossen JA, de Leeuw WJ, Molijn AC, Vijg J. Plasmid rescue from transgenic mouse DNA using LacI repressor protein conjugated to magnetic beads. Biotechniques. 1993 Apr;14(4):624-9. (Medline PMID: 8476606)
  • Dolle ME, Martus HJ, Gossen JA, Boerrigter ME, Vijg J. Evaluation of a plasmid-based transgenic mouse model for detecting in vivo mutations. Mutagenesis. 1996 Jan;11(1):111-8. (Medline PMID: 8671725)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
010589-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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