Strain Name:
B6J.Cg-Aprttm1Jat Tg(pUR288)1Vij/Mmmh
Stock Number:
010589-MU
Citation ID:
RRID:MMRRC_010589-MU
Major Collection:

Strain Information

Aprttm1Jat
Name: adenine phosphoribosyl transferase; targeted mutation 1, Jay A Tischfield
Synonyms: Arpt-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
Aprt
Name: adenine phosphoribosyl transferase
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Knockout
NCBI: 11821
HGNC: HGNC:626
Homologene: 413
pUR288
Name:
Type: Gene
Species: Bacteria
Chromosome:
Alteration at locus: Knockout
Tg(pUR288)1Vij
Name: transgene insertion 1, Jan Vijg
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (BAC)
Genetic Alterations
A positive-negative gene targeting vector, MBSF18, was constructed from 5.6 kb of mouse genomic DNA (strain 129?Sv) containing the entire coding sequence of Aprt. To verify the cloning, Southern blotting analysis was performed. Chimeric mice were produced by blastocyst injection using one D3 ES cell clone, MBSF1837. Chimeric mice were mated to Black Swiss. Offspring generated was tested for heterozygosity by Southern Blot.

LacZ Mice-200 copies of the pUR288 plasmid linearized with PstI were microinjected into fertilized C57BL/6J mouse oocytes. The offspring were analyzed for the presence of the transgene by Southern blot and bred to homozygosity.
Genotype Determination
ES Cell Line
D3 derived from 129S2/SvPas
Phenotype
The pUR288-lacZ transgenic mouse is used to detect a broad range of mutations, varying from point mutations in the lacZ gene to large genome rearrangements. However, this system cannot detect mutations involving gain or loss of entire lacZ copies. To detect loss of individual copies of the lacZ gene, the most suitable model is the heterozygous Aprt knockout mouse model (Engle et al., 1996), which can be applied to all tissues from which cells can be grown in short-term culture. This present Aprt/lacZ tool mouse model is a hybrid of the pUR288-lacZ and the Aprt knockout mouse. The model will allow the analysis of virtually all mutational events in a broad spectrum of tissues.
Mammalian Phenotype Terms
Allelic Composition: Aprttm1Jat/Aprttm1Jat (Genetic Background: involves: 129S2/SvPas * Black Swiss )

Allelic Composition: Aprttm1Jat/Aprttm1Jat (Genetic Background: involves: 129S2/SvPas * C57BL/6J )

Allelic Composition: Aprttm1Jat/Aprt+ (Genetic Background: involves: 129S2/SvPas * C57BL/6J )

MeSH Terms
  • Adenine/analogs & derivatives
  • Adenine/urine
  • Adenine Phosphoribosyltransferase/blood
  • Adenine Phosphoribosyltransferase/deficiency
  • Adenine Phosphoribosyltransferase/genetics
  • Alleles
  • Animals
  • Erythrocytes/enzymology
  • Fibrosis
  • Homozygote
  • Humans
  • Inflammation
  • Kidney/pathology
  • Kidney Calculi/genetics
  • Kidney Calculi/pathology
  • Kidney Calculi/urine
  • Mice
  • Mice, Knockout
  • Necrosis
  • Recombination, Genetic
  • Restriction Mapping
  • Stem Cells
  • Bacterial Proteins
  • Bacteriophage lambda/genetics
  • Biotechnology
  • DNA, Recombinant/genetics
  • DNA, Recombinant/isolation & purification
  • Escherichia coli/genetics
  • Escherichia coli Proteins
  • Evaluation Studies as Topic
  • Genetic Techniques
  • Genetic Vectors
  • Lac Operon
  • Lac Repressors
  • Magnetics
  • Mice, Transgenic
  • Plasmids/genetics
  • Plasmids/isolation & purification
  • Repressor Proteins
  • Ethylnitrosourea/toxicity
  • Galactose/genetics
  • Genes, Bacterial
  • Genes, Reporter
  • Mice, Inbred C57BL
  • Mice, Transgenic/genetics
  • Models, Genetic
  • Mutagens/toxicity
  • Mutation
  • Operon
Strain Development
LacZ animals harboring the lacZ gene chromosomally integrated head-to-tail on chromosome 11 were backcrossed with heterozygous animals for the Aprt allele. This cross was repeated 5 times until N5 generation was obtained.
Suggested Control Mice

Wild-type littermates

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Cell Biology
  • Models for Human Disease
  • Research Tools
Donor
Jan Vijg, Ph.D., Beth Israel Deaconess Medical Center
Primary Reference
  • Engle SJ, Stockelman MG, Chen J, Boivin G, Yum MN, Davies PM, Ying MY, Sahota A, Simmonds HA, Stambrook PJ, Tischfield JA. Adenine phosphoribosyltransferase-deficient mice develop 2,8-dihydroxyadenine nephrolithiasis. Proc Natl Acad Sci U S A. 1996 May 28;93(11):5307-12. (Medline PMID: 8643571)
  • Gossen JA, de Leeuw WJ, Molijn AC, Vijg J. Plasmid rescue from transgenic mouse DNA using LacI repressor protein conjugated to magnetic beads. Biotechniques. 1993 Apr;14(4):624-9. (Medline PMID: 8476606)
  • Dolle ME, Martus HJ, Gossen JA, Boerrigter ME, Vijg J. Evaluation of a plasmid-based transgenic mouse model for detecting in vivo mutations. Mutagenesis. 1996 Jan;11(1):111-8. (Medline PMID: 8671725)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
Generation
N5 (MMRRC:30330)
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Hetero/Hemizygotes are fertile: Yes Yes
Age Reproductive Decline: >12 mths 8 mths
Average litter size
7
Recommended wean age
3 weeks
Average Pups Weaned
7

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
010589-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.