Strain Name:
B6.129-Sgcbtm1Kcam/Mmmh
Stock Number:
010773-MU
Citation ID:
RRID:MMRRC_010773-MU

Gene Information

Sgcbtm1Kcam
Name: sarcoglycan, beta (dystrophin-associated glycoprotein); targeted mutation 1, Kevin P Campbell
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Knockout
Sgcb
Name: sarcoglycan, beta (dystrophin-associated glycoprotein)
Synonyms: beta-SG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Knockout
NCBI: 24051
Homologene: 195
Genetic Alterations
A targeting vector replaced exons three to six with phosphoglycerate kinase promoter/neomycin phosphotransferase cDNA by homologous recombination.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Only known mutation is replacement of exons three to six with phosphoglycerate kinase promoter/neomysin phosphotransferase cDNA.

Heterozygous: Heterzygotes do not show any known morphological abnormalities.
Mammalian Phenotype Terms
Allelic Composition: Sgcbtm1Kcam/Sgcbtm1Kcam (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

MeSH Terms
  • Animals
  • Cardiomyopathies/genetics
  • Cardiomyopathies/pathology
  • Cytoskeletal Proteins/deficiency
  • Cytoskeletal Proteins/genetics
  • Cytoskeletal Proteins/physiology
  • Dystroglycans
  • Dystrophin/genetics
  • Lung/pathology
  • Membrane Glycoproteins/deficiency
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/physiology
  • Mice
  • Mice, Knockout
  • Microsomes/pathology
  • Muscle, Skeletal/pathology
  • Muscular Dystrophy, Animal/genetics
  • Muscular Dystrophy, Animal/pathology
  • Myocardium/pathology
  • Necrosis
Strain Development
Original mouse on BL6/129 background. Since then they have been backcrossed 5 times onto C57/BL6.
Suggested Control Mice
  • Wildtype littermates
  • Cardiovascular
  • Developmental Biology
  • Models for Human Disease
  • Neurobiology
  • Musculoskeletal
Donor
Kevin P. Campbell, Ph.D., Howard Hughes Medical Institute
Primary Reference
Durbeej M, Cohn RD, Hrstka RF, Moore SA, Allamand V, Davidson BL, Williamson RA, Campbell KP. Disruption of the beta-sarcoglycan gene reveals pathogenetic complexity of limb-girdle muscular dystrophy type 2E. Mol Cell. 2000 Jan;5(1):141-51. (Medline PMID: 10678176)

Colony and Husbandry Information

Mice with decreased mobility may benefit from gruel as food/water source.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
010773-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
010773-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.