Strain Detail Sheet

Strain Name    :

B6.129-Asna1tm1Hbha/Mmnc

Stock Number :

010859-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Asna1tm1Hbha
Name: targeted mutation 1, Hiranmoy Bhattacharjee
Alteration at locus: Knockout
Gene Symbol: Asna1
Name: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Chromosome: 8
Alteration at locus: Knockout

Genetic Alterations:
The wild type Asna1 gene has 7-exons. The knockout construct has exon 1 and 2, truncated exon 6, and exon 7. The neo cassette is inserted between exon 2 and truncated exon 6.

Genotype Determination:

  • The genotyping protocol for this strain is currently not available. The MMRRC will develop a protocol if this strain is ordered.

ES Cell Line: R1 derived from 129X1/SvJ

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Homozygous knockout is embryonically lethal.

Heterozygous phenotype: Heterozygous knockout behaves similarly as the wild type strain.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Asna1tm1Hbha/Asna1tm1Hbha
        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • mortality/aging
    • complete embryonic lethality (MGI Ref ID J:110338)
      • die sometime between E3.5 and E8.5

Strain of Origin: 129X1/SvJ

Strain genetic background: C57BL/6

Strain Development: Blastocysts were harvested from 3.5 dpc C57BL/6 female mice that were naturally mated to C57BL/6 males. Recombinant ES cells were next injected into the blastocoel cavity of the blastocyst. Injected blastocysts were than transferred to the uterine horns of pseudopregnant CD-1 foster mothers. The chimeric male mice (the ES cells are derived from male mice) generated from blastocyst injection were bred with C57BL/6 female mice for determining germline transmission of the ES chromosomes. The agouti baby mice (ES cells were derived from 129SV mice which have agouti coat color which is dominant over the black coat color of C57BL/6 mice) were genotyped. The heterozygous knockout mice were next intercrossed (one male mouse housed with two female mice) to generate homozygous knockout mice.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Developmental Biology
  • Neurobiology
  • Research Tools
  • Toxicology

Strain Origin

Donor: Hiranmoy Bhattacharjee, Ph.D., Wayne State University School of Medicine

Primary Reference:

  • Bhattacharjee H, Ho YS, Rosen BP. Genomic organization and chromosomal localization of the Asna1 gene, a mouse homologue of a bacterial arsenic-translocating ATPase gene. Gene. 2001 Jul 11;272(1-2):291-9. (Medline PMID: 11470536)
  • Easterday MC, Dougherty JD, Jackson RL, Ou J, Nakano I, Paucar AA, Roobini B, Dianati M, Irvin DK, Weissman IL, Terskikh AV, Geschwind DH, Kornblum HI. Neural progenitor genes. Germinal zone expression and analysis of genetic overlap in stem cell populations. Dev Biol. 2003 Dec 15;264(2):309-22. (Medline PMID: 14651920)
  • Kurdi-Haidar B, Heath D, Aebi S, Howell SB. Biochemical characterization of the human arsenite-stimulated ATPase (hASNA-I). J Biol Chem. 1998 Aug 28;273(35):22173-6. (Medline PMID: 9712828)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
010859-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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