Strain Name:
B6.Cg-Get3tm1Hbha/Mmnc
Stock Number:
010859-UNC
Citation ID:
RRID:MMRRC_010859-UNC
Other Names:
B6.129-Asna1tm1Hbha/Mmnc

Strain Information

Get3tm1Hbha
Name: guided entry of tail-anchored proteins factor 3, ATPase; targeted mutation 1, Hiranmoy Bhattacharjee
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Knockout
Get3
Name: guided entry of tail-anchored proteins factor 3, ATPase
Synonyms: ArsA, 1810048H22Rik, Asna1
Type: Gene
Species: Mouse
Chromosome: 8
Alteration at locus: Knockout
NCBI: 56495
HGNC: HGNC:752
Homologene: 31513
Genetic Alterations
The wild-type Get3 gene has 7-exons. The knockout construct has exon 1 and 2, truncated exon 6, and exon 7. The neo cassette is inserted between exon 2 and truncated exon 6.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
    Phenotype
    Homozygous: Homozygous knockout is embryonically lethal.

    Heterozygous: Heterozygous knockout behaves similarly as the wild type strain.
    Mammalian Phenotype Terms
    Allelic Composition: Asna1tm1Hbha/Asna1tm1Hbha (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 )

    MeSH Terms
    • Adenosine Triphosphatases/genetics
    • Amino Acid Sequence
    • Animals
    • Arsenite Transporting ATPases
    • Base Sequence
    • Blotting, Northern
    • Blotting, Southern
    • Cell Line
    • Chromosome Banding
    • Chromosome Mapping
    • Cloning, Molecular
    • DNA/chemistry
    • DNA/genetics
    • DNA, Complementary/chemistry
    • DNA, Complementary/genetics
    • Exons
    • Genes/genetics
    • In Situ Hybridization, Fluorescence
    • Introns
    • Ion Pumps
    • Male
    • Mice
    • Mice, Inbred Strains
    • Molecular Sequence Data
    • Multienzyme Complexes
    • Phylogeny
    • RNA, Messenger/genetics
    • RNA, Messenger/metabolism
    • Regulatory Sequences, Nucleic Acid
    • Sequence Alignment
    • Sequence Analysis, DNA
    • Sequence Homology, Amino Acid
    • Tissue Distribution
    • Brain/cytology
    • Brain/metabolism
    • Cells, Cultured
    • Embryo, Mammalian/cytology
    • Embryo, Mammalian/embryology
    • Embryo, Mammalian/metabolism
    • Gene Expression Profiling
    • Hematopoietic Stem Cells/metabolism
    • In Situ Hybridization
    • Neurons/cytology
    • Oligonucleotide Array Sequence Analysis
    • Stem Cells/metabolism
    • Adenosine Triphosphatases/metabolism
    • Adenosine Triphosphate/metabolism
    • Arsenites/pharmacology
    • Blotting, Western
    • Cross-Linking Reagents
    • Enzyme Activation
    • Glutathione Transferase/chemistry
    • Humans
    • Protein Binding
    • Recombinant Fusion Proteins/chemistry
    • Recombinant Fusion Proteins/metabolism
    Strain Development
    Blastocysts were harvested from 3.5 dpc C57BL/6 female mice that were naturally mated to C57BL/6 males. Recombinant ES cells, R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+) were next injected into the blastocoel cavity of the blastocyst. Injected blastocysts were then transferred to the uterine horns of pseudopregnant CD-1 foster mothers. The chimeric male mice generated from blastocyst injection were bred with C57BL/6 female mice for determining germline transmission of the ES chromosomes. The agouti baby mice (due to 129/Sv contribution) were genotyped. The heterozygous knockout mice were intercrossed (1 male x 2 female) to generate homozygotes..
    Suggested Control Mice

    Wild-type littermates

    MMRRC Genetic QC Summary
    Genetic QC analysis (GQC) was performed on representative samples from the donor submission and provides a baseline reference. Regardless of the material ordered (live mice, sperm, embryos, resuscitated animals), the provided material is guaranteed to contain the primary genetic alteration of interest; however, the composition of the genetic background may vary (See Reproducibility sections of the report for details). The impact of genetic background on previously reported phenotypes has not been evaluated.

    GQC analysis was done on two XY samples VM8959 and JA1465 at the time of importation and the conclusions are consistent in both samples. The results of the analysis are congruent with the SDS and VM8959 is the representative sample selected for the report.

    This sample has more than 2 genetic backgrounds (unexplained regions and/or fractured ideogram). The strain selected for secondary background may be incorrect. The estimation of the contribution of primary and secondary background are likely incorrect. None of the contructs detectable by MiniMUGA are present. The estimated inbreeding, congenicity, and the relative strain/substrain contribution depend on the number of samples genotyped. The genomes of strains that are not fully inbred are not completely reproducible.
    MiniMUGA Sample Report
    • Developmental Biology
    • Neurobiology
    • Research Tools
    • Toxicology
    Donor
    Hiranmoy Bhattacharjee, Wayne State University.
    Primary Reference

    Bhattacharjee H, Ho YS, Rosen BP. Genomic organization and chromosomal localization of the Asna1 gene, a mouse homologue of a bacterial arsenic-translocating ATPase gene. Gene. 2001 Jul 11;272(1-2):291-9. (Medline PMID: 11470536)

    Easterday MC, Dougherty JD, Jackson RL, Ou J, Nakano I, Paucar AA, Roobini B, Dianati M, Irvin DK, Weissman IL, Terskikh AV, Geschwind DH, Kornblum HI. Neural progenitor genes. Germinal zone expression and analysis of genetic overlap in stem cell populations. Dev Biol. 2003 Dec 15;264(2):309-22. (Medline PMID: 14651920)

    Kurdi-Haidar B, Heath D, Aebi S, Howell SB. Biochemical characterization of the human arsenite-stimulated ATPase (hASNA-I). J Biol Chem. 1998 Aug 28;273(35):22173-6. (Medline PMID: 9712828)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    010859-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    010859-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.