Allele Symbol: Asna1tm1Hbha
Name: targeted mutation 1, Hiranmoy Bhattacharjee
Alteration at locus: Knockout
Gene Symbol: Asna1
Name: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Alteration at locus: Knockout
ES Cell Line: R1 derived from 129X1/SvJ
Homozygous phenotype: Homozygous knockout is embryonically lethal.
Heterozygous phenotype: Heterozygous knockout behaves similarly as the wild type strain.
Strain of Origin: 129X1/SvJ
Strain genetic background: C57BL/6
Strain Development: Blastocysts were harvested from 3.5 dpc C57BL/6 female mice that were naturally mated to C57BL/6 males. Recombinant ES cells were next injected into the blastocoel cavity of the blastocyst. Injected blastocysts were than transferred to the uterine horns of pseudopregnant CD-1 foster mothers. The chimeric male mice (the ES cells are derived from male mice) generated from blastocyst injection were bred with C57BL/6 female mice for determining germline transmission of the ES chromosomes. The agouti baby mice (ES cells were derived from 129SV mice which have agouti coat color which is dominant over the black coat color of C57BL/6 mice) were genotyped. The heterozygous knockout mice were next intercrossed (one male mouse housed with two female mice) to generate homozygous knockout mice.
Suggested Control Mice:
Donor: Hiranmoy Bhattacharjee, Ph.D., Wayne State University School of Medicine
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.