Allele Symbol: Card11unm
Alteration at locus: Chemically Induced
Gene Symbol: Card11
Name: caspase recruitment domain family, member 11
Alteration at locus: Chemically Induced
ES Cell Line: Not applicable
Homozygous phenotype: B & T cell response defects; atopic dermatitis;
high IgM on IgD+ cells, also slightly increased CD21; reduced peritoneal
B1 cells; severely affected immunity mediated by Th1 cells, selective defects
in BCR and TCR signalling to JNK and NFkB. As mice age they develop
atopic dermatitis and hyper-IgE, with red, itchy ears and weepy eyes, progressing
to badly eroded skin from itching, at which point the animals need to be
Heterozygous phenotype: Normal
Strain of Origin: C57BL/6
Strain genetic background: C57BL/6
Strain Development: ENU-induced mutation in a male C57BL/6.
Suggested Control Mice:
Donor: Chris Goodnow, BVSc, PhD, The Australian National University, Canberra, Australia
Jun JE, Wilson LE, Vinuesa CG, Lesage S, Blery M, Miosge LA, Cook MC, Kucharska EM, Hara H, Penninger JM, Domashenz H, Hong NA, Glynne RJ, Nelms KA, Goodnow CC. Identifying the MAGUK protein Carma-1 as a central regulator of humoral immune responses and atopy by genome-wide mouse mutagenesis. Immunity. 2003 Jun;18(6):751-62. (Medline PMID: 12818157)
Coat color: Black
MMRRC Breeding System: Backcross or Sib-mated
Overall Breeding Performance: Poor
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Reduced||Reduced|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Unknown||Unknown|
Average litter size: 5
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Some MMRRC strains were submitted by the donor as cryopreserved germplasm, see Fees section for further details.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.