Strain Name    : C57BL/6-Card11unm/ApbMmucd

Stock Number : 011120-UCD

Other Names   : Unmodulated, C57BL/6-Card11unm/Mmcd

Gene Information

(provided by MGI)
Allele Symbol: Card11unm
Name: unmodulated
Alteration at locus: Chemically Induced
Gene Symbol: Card11
Name: caspase recruitment domain family, member 11
Chromosome: 5
Alteration at locus: Chemically Induced
Genetic Alterations:
The Unmodulated mutation corresponds to an ENU-induced T to A transversion at position 1235 in exon 7. This mutation results in a Leucine to Glutamine amino acid change at position 305.

Genotype Determination:

ES Cell Line: Not Applicable

Phenotype

Homozygous phenotype: B & T cell response defects; atopic dermatitis; high IgM on IgD+ cells, also slightly increased CD21; reduced peritoneal B1 cells; severely affected immunity mediated by Th1 cells, selective defects in BCR and TCR signalling to JNK and NFkB. As mice age they develop atopic dermatitis and hyper-IgE, with red, itchy ears and weepy eyes, progressing to badly eroded skin from itching, at which point the animals need to be put down.

Heterozygous phenotype: Normal


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Card11unm/Card11unm
        C57BL/6
  • hematopoietic system phenotype
    • abnormal B cell differentiation (MGI Ref ID J:84042)
      • shift to higher levels of surface IgM and CD21
      • antigen responses decreased or absent
      • Th1 type antibody response more affected than Th2 type response
      • lower responses to B cell activating stimuli
      • B cell responses selectively impaired
    • abnormal T cell activation (MGI Ref ID J:84042)
      • defective CD28 costimulation of T cells but T cell activation otherwise normal
    • decreased B-1 B cell number (MGI Ref ID J:84042)
      • peritoneal B1 cells are nearly absent but B2 cells unaffected
    • spleen hyperplasia (MGI Ref ID J:84042)
      • increased cellularity in spleen
      • frequencies of T and B cells in spleen normal
  • immune system phenotype
    • abnormal B cell differentiation (MGI Ref ID J:84042)
      • shift to higher levels of surface IgM and CD21
      • antigen responses decreased or absent
      • Th1 type antibody response more affected than Th2 type response
      • lower responses to B cell activating stimuli
      • B cell responses selectively impaired
    • abnormal immune system physiology (MGI Ref ID J:84042)
      • abnormal T cell activation (MGI Ref ID J:84042)
        • defective CD28 costimulation of T cells but T cell activation otherwise normal
      • decreased immunoglobulin level (MGI Ref ID J:84042)
        • decreased IgG3 level (MGI Ref ID J:84042)
          • 90% reduction in IgG3 levels in serum
          • overall IgG levels about normal
        • decreased IgM level (MGI Ref ID J:84042)
          • 50% decrease in IgM levels in serum
      • increased immunoglobulin level (MGI Ref ID J:84042)
        • increased IgE level (MGI Ref ID J:84042)
          • high serum levels develop with age
      • increased inflammatory response (MGI Ref ID J:84042)
        • teary eyes
        • dermatitis (MGI Ref ID J:84042)
          • dermatitis develops on ears and neck
          • hyperkeratosis of inflamed regions of skin
          • erythroderma (MGI Ref ID J:84042)
            • erythematous ears at 10-20 weeks
            • become intensely itchy
    • decreased B-1 B cell number (MGI Ref ID J:84042)
      • peritoneal B1 cells are nearly absent but B2 cells unaffected
    • lymph node hyperplasia (MGI Ref ID J:84042)
      • increased cellularity in lymph nodes
      • frequencies of T and B cells in lymph nodes normal
    • spleen hyperplasia (MGI Ref ID J:84042)
      • increased cellularity in spleen
      • frequencies o f T and B cells in spleen normal
  • integument phenotype
    • dermatitis (MGI Ref ID J:84042)
      • dermatitis develops on ears and neck
      • hyperkeratosis of inflamed regions of skin
      • erythroderma (MGI Ref ID J:84042)
        • erythematous ears at 10-20 weeks
        • become intensely itchy


Strain of Origin: C57BL/6

Strain genetic background: C57BL/6

Strain Development: ENU-induced mutation in a male C57BL/6.

Suggested Control Mice:

  • Wild-type littermates

  • Cell biology
  • Dermatology
  • Developmental biology
  • Immunology and inflammation

Donor: Chris Goodnow, BVSc, PhD, The Australian National University, Canberra, Australia

Primary Reference:

Jun JE, Wilson LE, Vinuesa CG, Lesage S, Blery M, Miosge LA, Cook MC, Kucharska EM, Hara H, Penninger JM, Domashenz H, Hong NA, Glynne RJ, Nelms KA, Goodnow CC. Identifying the MAGUK protein Carma-1 as a central regulator of humoral immune responses and atopy by genome-wide mouse mutagenesis. Immunity. 2003 Jun;18(6):751-62. (Medline PMID: 12818157)

Colony and Husbandry Information

As mice age they develop atopic dermatitis, progressing to badly eroded skin from itching, at which point the animals need to be put down.

This strain is also reposited as Unmodulated at the NHMRC Australian PhenomeBank (APB).

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Coat color: Black

Other:


MMRRC Breeding System: Backcross or Sib-mated

Breeding Scheme(s):

    Generation:

    Overall Breeding Performance: Poor

    Viability and Fertility:FemaleMaleComments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Reduced Reduced
    Heterozygotes are fertile: Yes Yes
     
    Age Reproductive Decline: Unknown Unknown

    Average litter size: 5

    Recommended wean age: 3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, see Fees section for further details.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    011120-UCD-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    011120-UCD-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2
    011120-UCD-EMBRYOCryo-preserved embryos
    $1,038.00
    Non-Profit
    Aliquot Approximate quantity3: 20-40 embryos / aliquot

    Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.