Strain Name:
Stock Number:
Citation ID:

Gene Information

Name: tumor necrosis factor (ligand) superfamily, member 4; targeted mutation 1, Arlene H Sharpe
Synonyms: OX40Lo, Ox40L-/-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
Name: tumor necrosis factor (ligand) superfamily, member 4
Synonyms: Ath-1, OX40L, TXGP1, gp34, Txgp1l, Ath1, CD134L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Knockout
NCBI: 22164
Homologene: 2495
Genetic Alterations
neo gene replaces entire extracellular dumain and part of 3' untranslated region of OX40L
Genotype Determination
ES Cell Line
J1 derived from 129S4/SvJae
Homozygous: defect in T cell activation

Heterozygous: same as wild-type
Mammalian Phenotype Terms
Allelic Composition: Tnfsf4tm1Shr/Tnfsf4tm1Shr (Genetic Background: C.129S4-Tnfsf4tm1Shr )

Allelic Composition: Tnfsf4tm1Shr/Tnfsf4tm1Shr (Genetic Background: involves: 129S4/SvJae )

Allelic Composition: Tnfsf4tm1Shr/Tnfsf4tm1Shr (Genetic Background: NOD.129S4-Tnfsf4tm1Shr )

MeSH Terms
  • 3T3 Cells
  • Animals
  • Antigens, T-Independent/immunology
  • Dendritic Cells/immunology
  • Dermatitis, Contact/immunology
  • Haptens/immunology
  • Hemocyanins/immunology
  • Hypersensitivity, Delayed/immunology
  • Membrane Glycoproteins
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Ovalbumin/immunology
  • Receptors, Tumor Necrosis Factor/genetics
  • Receptors, Tumor Necrosis Factor/immunology
  • T-Lymphocytes/immunology
  • Tumor Necrosis Factors
Strain Development
Chimeric mice were bred with 129WT mice (same strain as J1ES cell). So mice are inbred 129SvS4Jae.
Suggested Control Mice
  • Wildtype littermates
  • Immunology and Inflammation
Arlene Sharpe, Ph.D., Harvard Medical School
Primary Reference
Chen AI, McAdam AJ, Buhlmann JE, Scott S, Lupher ML Jr, Greenfield EA, Baum PR, Fanslow WC, Calderhead DM, Freeman GJ, Sharpe AH. Ox40-ligand has a critical costimulatory role in dendritic cell:T cell interactions. Immunity. 1999 Dec;11(6):689-98. (Medline PMID: 10626891)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
011140-MU-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
011140-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity2
011140-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.