Strain Detail Sheet

Strain Name    :

C3Fe.Cg-Mcoln3Va-J/Mmmh

Stock Number :

011149-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Mcoln3Va-J
Name: varitint waddler Jackson
Alteration at locus: Spontaneous Mutation
Gene Symbol: Mcoln3
Name: mucolipin 3
Chromosome: 3
Alteration at locus: Spontaneous Mutation

Genetic Alterations:
VaJ is a spontaneous mutation that occurred in the Mcoln3 locus on chromosome 3.

Genotype Determination:

ES Cell Line:

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

The strain is a coat color mutant and the genotype of the heterozygotes can be tracked by their appearance of a white spot at the belly and the forehead; the homozygotes are clearly white with a few dark patches.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Mcoln3/?
        involves: A/J * C57BL/6J
  • hearing/vestibular/ear phenotype
    • absent brainstem auditory evoked potential (MGI Ref ID J:78812)
      • at 12 weeks of age F2 progeny of a cross to A/J fail to respond to a 16 kHz stimulus at >100 dB, indicating that there are no background modifiers ameliorating this phenotype
Mcoln3/?
        involves: BALB/cByJ * C57BL/6J
  • hearing/vestibular/ear phenotype
    • absent brainstem auditory evoked potential (MGI Ref ID J:78812)
      • at 12 weeks of age F2 progeny of a cross to BALB/cByJ fail to respond to a 16 kHz stimulus at >100 dB, indicating that there are no background modifiers ameliorating this phenotype
Mcoln3/?
        involves: C3HeB/FeJLe * C57BL/6J
  • hearing/vestibular/ear phenotype
    • absent brainstem auditory evoked potential (MGI Ref ID J:78812)
      • at 12 weeks of age F2 progeny of a cross to C3HeB/FeJLe fail to respond to a 16 kHz stimulus at >100 dB, indicating that there are no background modifiers ameliorating this phenotype
Mcoln3/?
        involves: C57BL/6J * CZECHII/EiJ
  • hearing/vestibular/ear phenotype
    • absent brainstem auditory evoked potential (MGI Ref ID J:78812)
      • at 12 weeks of age F2 progeny of a cross to CZECHII/EiJ fail to respond to a 16 kHz stimulus at >100 dB, indicating that there are no background modifiers ameliorating this phenotype
Mcoln3/?
        involves: C57BL/6J * DBA/2 J
  • hearing/vestibular/ear phenotype
    • absent brainstem auditory evoked potential (MGI Ref ID J:78812)
      • at 12 weeks of age F2 progeny of a cross to DBA/2J fail to respond to a 16 kHz stimulus at >100 dB, indicating that there are no background modifiers ameliorating this phenotype
Mcoln3Va-J/Mcoln3+
        B6C3Fe-a/a Hoxa13<Hd> Mcoln3<Va-J>/J
  • pigmentation phenotype
    • belly spot (MGI Ref ID J:78812)
      • heterozygotes express a small white ventral spot
    • diluted coat color (MGI Ref ID J:78812)
      • heterozygotes express a slightly diluted coat color which is less diluted than that of the original veritant waddler heterozygotes
    • head spot (MGI Ref ID J:78812)
      • some heterozygotes have a white forehead patch
  • hearing/vestibular/ear phenotype
    • abnormal brainstem auditory evoked potential (MGI Ref ID J:78812)
      • at 2 weeks of age heterozygotes respond to 8- and 16-kHz stimuli only at sound pressure levels of 88 dB and 94 dB respectively, no response was found to click or 32 kHz stimuli, and at 3 weeks of age thresholds increased to >100 dB, which, although it is an early and rapidly progressing hearing loss, is a less severe phenotype than in mice heterozygous for the original veritant waddler mutation
      • absent brainstem auditory evoked potential (MGI Ref ID J:78812)
      • decreased brainstem auditory evoked potential (MGI Ref ID J:78812)
  • integument phenotype
    • belly spot (MGI Ref ID J:78812)
      • heterozygotes express a small white ventral spot
    • diluted coat color (MGI Ref ID J:78812)
      • heterozygotes express a slightly diluted coat color which is less diluted than that of the original veritant waddler heterozygotes
    • head spot (MGI Ref ID J:78812)
      • some heterozygotes have a white forehead patch
Mcoln3Va-J/Mcoln3+
        Background Not Specified
  • hearing/vestibular/ear phenotype
  • behavior/neurological phenotype
    • behavior/neurological phenotype (MGI Ref ID J:64107)
      • behave normally unlike Mcoln3Va
  • pigmentation phenotype
    • abnormal skin pigmentation (MGI Ref ID J:5286)
      • white feet
      • non-pigmented tail tip (MGI Ref ID J:5286)
    • belly spot (MGI Ref ID J:5286)
      • mice exhibit a large, irregular belly spot
    • diluted coat color (MGI Ref ID J:5286)
    • variegated coat color (MGI Ref ID J:64107)
      • a slightly dilute coat color, large irregular belly spots and white feet and tail tips
      • not as varicolored as unlike Mcoln3Va
  • limbs/digits/tail phenotype
    • non-pigmented tail tip (MGI Ref ID J:5286)
  • integument phenotype
    • abnormal skin pigmentation (MGI Ref ID J:5286)
      • white feet
      • non-pigmented tail tip (MGI Ref ID J:5286)
    • belly spot (MGI Ref ID J:5286)
      • mice exhibit a large, irregular belly spot
    • diluted coat color (MGI Ref ID J:5286)
    • variegated coat color (MGI Ref ID J:64107)
      • a slightly dilute coat color, large irregular belly spots and white feet and tail tips
      • not as varicolored as unlike Mcoln3Va
Mcoln3Va-J/Mcoln3Va-J
        B6.Cg-Mcoln3<Va-J>
  • mortality/aging
    • partial prenatal lethality (MGI Ref ID J:78812)
      • at backcross generation N6 to C57BL/6J intercrossing heterozygotes yields only 8% homozygous offspring
Mcoln3Va-J/Mcoln3Va-J
        B6C3Fe-a/a Hoxa13<Hd> Mcoln3<Va-J>/J
  • pigmentation phenotype
    • mottled coat (MGI Ref ID J:78812)
      • homozygotes are almost entirely variegated both ventrally and dorsally
  • integument phenotype
    • mottled coat (MGI Ref ID J:78812)
      • homozygotes are almost entirely variegated both ventrally and dorsally
Mcoln3Va-J/Mcoln3Va-J
        Background Not Specified
  • hearing/vestibular/ear phenotype
  • behavior/neurological phenotype
    • behavior/neurological phenotype (MGI Ref IDs J:5286, J:64107)
      • both sexes are fertile and do not have the abnormal behavior of Mcoln3Va (MGI Ref ID J:64107)
      • able to swim (MGI Ref ID J:64107)
      • mice swim and behave normally (MGI Ref ID J:5286)
  • pigmentation phenotype
    • diluted coat color (MGI Ref ID J:5286)
    • variegated coat color (MGI Ref ID J:64107)
      • mostly white with patches of dilute color
    • white spotting (MGI Ref ID J:5286)
  • integument phenotype
    • diluted coat color (MGI Ref ID J:5286)
    • variegated coat color (MGI Ref ID J:64107)
      • mostly white with patches of dilute color
    • white spotting (MGI Ref ID J:5286)

Strain of Origin: B6C3Fe-a/a-Mcoln3

Strain genetic background: C3HeB/FeJ

Strain Development: Strain B6C3Fe-a/a Mcoln3/+ (JAX stock # 000296) was backcrossed to C3HeB/FeJ. Heterozygous carriers were identified by their white spot on the belly and forehead.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Developmental Biology
  • Sensorineural
  • Neural crest, melanocyte differentiation/survival

Strain Origin

Donor: Konrad Noben-Trauth, Ph.D., NIH, National Institute on Deafness and Other Communication Disorders

Primary Reference:

Di Palma F, Belyantseva IA, Kim HJ, Vogt TF, Kachar B, Noben-Trauth K. Mutations in Mcoln3 associated with deafness and pigmentation defects in varitint-waddler (Va) mice. Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14994-9. Epub 2002 Oct 25. (Medline PMID: 12403827)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
011149-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
011149-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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