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Strain Name:
B6.D2-Tg(Ins2-cre)23Herr/Mmmh
Stock Number:
011155-MU
Citation ID:
RRID:MMRRC_011155-MU

Strain Information

cre
Name: cre recombinase
Type: Gene
Species: Enterobacteria phage P1 (bacteriophage P1)
Chromosome:
Ins2
Name: insulin II
Synonyms: Ins-2, Mody4, Mody, InsII
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16334
HGNC: HGNC:6081
Homologene: 173
Tg(Ins2-cre)23Herr
Name: transgene insertion 23, Pedro Luis Herrera
Synonyms: Ins(cre), InsPr-Cre, (INSPr)-Cre, RipCre, RIP-CreHerr, InsCre, RIP-cre
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: unknown
Alteration at locus: Transgenic (cre)
Genetic Alterations
A 0.6 kb fragment containing the rat insulin promoter was adjoined to the cre recombinase coding sequence to drive expression in pancreatic cells.
Genotype Determination
Phenotype
Recombination of floxed DNA in insulin-producing beta cells
MeSH Terms
  • Animals
  • Cell Differentiation
  • Cell Lineage
  • Female
  • Gene Expression
  • Glucagon/biosynthesis
  • Glucagon/genetics
  • Homeodomain Proteins/genetics
  • Humans
  • Insulin/biosynthesis
  • Insulin/genetics
  • Islets of Langerhans/cytology
  • Islets of Langerhans/metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Mice, Transgenic
  • Pancreatic Polypeptide/genetics
  • Stem Cells/cytology
  • Stem Cells/metabolism
  • Trans-Activators/genetics
Strain Development
After generation, the mice were backcrossed to C57BL/6 for 11 generations.
Suggested Control Mice
Wild-type littermates, C57BL/6
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Developmental Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Metabolism
  • Models for Human Disease
Donor
Pedro Herrera, Ph.D., University of Geneva Medical School
Primary Reference
Herrera PL. Adult insulin- and glucagon-producing cells differentiate from two independent cell lineages. Development. 2000 Jun;127(11):2317-22. (Medline PMID: 10804174)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
011155-MU-SPERM Cryo-preserved spermatozoa $437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity3
011155-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.