Allele Symbol: Chrm1tm1Nmn
Name: targeted mutation 1, Neil M Nathanson
Alteration at locus: Knockout
Gene Symbol: Chrm1
Name: cholinergic receptor, muscarinic 1, CNS
Alteration at locus: Knockout
ES Cell Line: REK3 derived from 129X1/SvJ
Homozygous phenotype: No overt phenotype. Mice show resistance to certain types of seizures, deficits in cognition and
neuroplasticity, alterations in regulation o signal transduction and ion channel regulatory pathways.
Heterozygous phenotype: Heterozygotes are nearly as resistant as homozygotes to pilocarpine-induced seizures.
Strain of Origin: 129SvJ
Strain genetic background: C57BL/6
Strain Development: Chimeric mice were crossed to C57BL/6; heterozygotes (alternating male and females in successive
crosses) were mated to C57BL/6. Mice were originally generated in a conventional (non-SPF)
facility, and were cross-fostered into a barrier-SPF facility ~ 1996.
Strain Genetic Background Validation:
A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.
|MMRRC Strain||Reference Strains||Genomic makeup (Mb)|
|Strain Name||MMRRC ID #||Reference 1||Reference 2||Ref 1||Ref 2||Het||Unknown|
Suggested Control Mice:
Donor: Neil M. Nathanson, Ph.D., University of Washington
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.