Strain Detail Sheet

Strain Name    :

B6.129X1-Chrm1tm1Nmn/Mmnc

Stock Number :

011172-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Chrm1tm1Nmn
Name: targeted mutation 1, Neil M Nathanson
Alteration at locus: Knockout
Gene Symbol: Chrm1
Name: cholinergic receptor, muscarinic 1, CNS
Chromosome: 19
Alteration at locus: Knockout

Genetic Alterations:
A 0.3 kb KpnI-SStI fragment containing 5'-noncoding sequence and 165 bp of M1 receptor coding sequence was replaced with a neo casette.

Genotype Determination:

ES Cell Line: REK3 derived from 129X1/SvJ

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: No overt phenotype. Mice show resistance to certain types of seizures, deficits in cognition and neuroplasticity, alterations in regulation o signal transduction and ion channel regulatory pathways.
Heterozygous phenotype: Heterozygotes are nearly as resistant as homozygotes to pilocarpine-induced seizures.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Chrm1tm1Nmn/Chrm1tm1Nmn
        B6.129X1-Chrm1<tm1Nmn>
  • nervous system phenotype
    • abnormal CNS synapse formation (MGI Ref ID J:96239)
      • the dendritic arborization of layer IV multi-polar granular neurons is markedly reduced, suggesting impaired synapse formation
    • abnormal auditory cortex morphology (MGI Ref ID J:96239)
      • at 6-8 weeks of age, homozygotes display a grossly normal architecture in all six layers of the auditory cortex, with no differences in total dendrite length of layer V pyramidal neurons
      • however, the dendritic arborization of layer IV multi-polar granular neurons is markedly reduced, with dendrites being shorter and sparser than normal, suggesting a deficit in thalamocortical innervation or synapse formation
      • abnormal auditory cortex tonotopy (MGI Ref ID J:96239)
        • at 6-8 weeks of age, homozygotes display disorganized frequency maps (tonotopy) of the auditory cortex due to deficits in frequency tuning of cortical neurons and impaired cytoarchitecture in layer IV of the auditory cortex
        • in contrast, tonotopic organization and frequency tuning in the auditory midbrain is normal
  • hearing/vestibular/ear phenotype
    • hearing/vestibular/ear phenotype (MGI Ref ID J:96239)
      • at 6-8 weeks of age, homozygotes exhibit normal hearing sensitivity, with similar auditory brainstem response (ABR) thresholds across various frequencies ranging from 3 to 36 kHz relative to wild-type mice

Strain of Origin: 129SvJ

Strain genetic background: C57BL/6

Strain Development: Chimeric mice were crossed to C57BL/6; heterozygotes (alternating male and females in successive crosses) were mated to C57BL/6. Mice were originally generated in a conventional (non-SPF) facility, and were cross-fostered into a barrier-SPF facility ~ 1996.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6.129X1-Chrm1tm1Nmn/Mmnc MMRRC:011172-UNC C57BL/6J 129X1 2562 4 15 0


Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
  • Immunology and Inflammation
  • Neurobiology
  • Sensorineural

Strain Origin

Donor: Neil M. Nathanson, Ph.D., University of Washington

Primary Reference:

  • Hamilton SE, Loose MD, Qi M, Levey AI, Hille B, McKnight GS, Idzerda RL, Nathanson NM. Disruption of the m1 receptor gene ablates muscarinic receptor-dependent M current regulation and seizure activity in mice. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13311-6. (Medline PMID: 9371842)
  • Hamilton SE, Nathanson NM. The M1 receptor is required for muscarinic activation of mitogen-activated protein (MAP) kinase in murine cerebral cortical neurons. J Biol Chem. 2001 May 11;276(19):15850-3. Epub 2001 Feb 15. (Medline PMID: 11278934)
  • Anagnostaras SG, Murphy GG, Hamilton SE, Mitchell SL, Rahnama NP, Nathanson NM, Silva AJ. Selective cognitive dysfunction in acetylcholine M1 muscarinic receptor mutant mice. Nat Neurosci. 2003 Jan;6(1):51-8. (Medline PMID: 12483218)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Use of MICE by companies or for-profit entities requires a license from The University of Washington. Contact filimus@u.washington.edu.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
011172-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
011172-UNC-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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