Strain Detail Sheet

Strain Name    :

B6.Cg-Tg(CAG-OTC/CAT)4033Prab/Mmmh

Stock Number :

011195-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(CAG-OTC/CAT)4033Prab
Name: transgene insertion 4033, Peter S Rabinovitch
Alteration at locus: Transgenic
Transgene: CAT (Human)
Name: catalase
Alteration at locus: Transgenic
Promoter: CAG (Chicken/Rabbit)
Name: CMV early enhancer/chicken beta actin promoter/rabbit beta globin splice acceptor
Alteration at locus: Transgenic

Genetic Alterations:
To generate the MCAT transgene, the 11 carboxy-terminal amino acids, including the peroxisomal localization signal, were deleted from the human catalase gene. The initiating methionine was also deleted and the first 25 amino acids of the ornithine transcarbamylase leader sequence were added to the amino terminus to target this protein product to mitochondria. The catalase cDNA construct was then cloned into a plasmid under the influence of the chicken-actin promoter/CMV enhancer (CAG) and the construct was introduced into B6C3H X B6 hybrid embryos.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Unknown

Hemizygous phenotype: Transgenic mice with targeted overexpression of catalase in mitochondria show lifespan extension, reduced aging pathology and reduced susceptibility to oxidative damage. Median and maximum life spans were maximally increased (average 5 months, and 5.5 months, respectively) in MCAT animals. Cardiac pathology and cataract development were delayed, oxidative damage (8-hydroxyguanosine) was reduced, H2O2 production and H2O2-induced aconitase inactivation were attenuated, and the development of mitochondrial deletions measured by LX-PCR was reduced.

Strain of Origin: B6C3H x B6 hybrid

Strain genetic background: C57BL/6

Strain Development: G1 - G12 mCAT Tg male x Bl/6 female G13 mCAT Tg male x mCAT Tg female to check congenity on Bl/6 background G14 mCAT Tg male x Bl/6 female

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cardiovascular
  • Diabetes/Obesity
  • Neurobiology
  • Oxidative Stress

Strain Origin

Donor: Warren C. Ladiges, DVM, University of Washington

Primary Reference:

Schriner SE, Linford NJ, Martin GM, Treuting P, Ogburn CE, Emond M, Coskun PE, Ladiges W, Wolf N, Van Remmen H, Wallace DC, Rabinovitch PS. Extension of murine life span by overexpression of catalase targeted to mitochondria. Science. 2005 Jun 24;308(5730):1909-11. Epub 2005 May 5. (Medline PMID: 15879174)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
011195-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
011195-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
011195-MU-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

Generated: 04/16/2014