Strain Name:
B6.129-Utrntm1Jrs/Mmucd
Stock Number:
011196-UCD
Citation ID:
RRID:MMRRC_011196-UCD
Other Names:
Utrophin, B6.129-Utrntm1Jrs/Mmcd

Strain Information

Utrntm1Jrs
Name: utrophin; targeted mutation 1, Joshua R Sanes
Synonyms: utrn-, uko
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Knockout
Utrn
Name: utrophin
Synonyms: G-utrophin, DRP, Dmdl
Type: Gene
Species: Mouse
Chromosome: 10
Alteration at locus: Knockout
NCBI: 22288
VEGA: 10
Homologene: 21398
Genetic Alterations
a neo cassette was inserted into the utrophin exon that encodes the beginning of the cystein-rich domain
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: Utrophin null mice are normal in appearance and behavior and show no obvious defects in muscle or non-muscle tissue.

Heterozygous: Heterozygotes do not show any known morphological abnormalities.
Mammalian Phenotype Terms
Allelic Composition: Utrntm1Jrs/Utrntm1Jrs (Genetic Background: involves: 129S1/Sv * 129X1/SvJ )

Allelic Composition: Utrntm1Jrs/Utrntm1Jrs (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 )

MeSH Terms
  • Animals
  • Cardiomyopathies/complications
  • Cardiomyopathies/pathology
  • Cytoskeletal Proteins/deficiency
  • Disease Models, Animal
  • Dystrophin/deficiency
  • Female
  • Male
  • Membrane Proteins/deficiency
  • Mice
  • Mice, Inbred mdx
  • Muscle Contraction
  • Muscle, Skeletal/pathology
  • Muscle, Skeletal/physiology
  • Muscular Dystrophy, Animal/complications
  • Muscular Dystrophy, Animal/pathology
  • Myocardium/pathology
  • Neuromuscular Junction/pathology
  • Regeneration
  • Utrophin
  • Cytoskeletal Proteins/genetics
  • Immunohistochemistry
  • Membrane Proteins/genetics
  • Mice, Mutant Strains
  • Muscle, Skeletal/chemistry
  • Muscle, Skeletal/metabolism
  • Muscle, Skeletal/physiopathology
  • Neuromuscular Diseases/genetics
  • Neuromuscular Diseases/metabolism
  • Neuromuscular Diseases/physiopathology
  • Neuromuscular Junction/metabolism
  • Neuromuscular Junction/ultrastructure
  • Organ Specificity/genetics
  • Receptors, Cholinergic/metabolism
  • Synaptic Vesicles/metabolism
  • Synaptic Vesicles/ultrastructure
Strain Development
Chimeric mice were bred to C57BL/6 and mice heterozygous for the mutant allele were selected by genotyping.

These heterozygotes were bred together to produce homozygotes. Repeated backcrosses to C57BL/6 were made by breeding the homozygous null mouse with a C57BL/6 mouse. The heterozygous mice generated from this breeding scheme were bred together to produce homozyotes. This breeding scheme was repeated 6 times in order to increase the purity ot the C57BL/6 background.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
  • Models for Human Disease
  • Musculoskeletal
Donor
Joshua Sanes, Ph.D., Harvard University
Primary Reference
  • Grady RM, Teng H, Nichol MC, Cunningham JC, Wilkinson RS, Sanes JR. Skeletal and cardiac myopathies in mice lacking utrophin and dystrophin: a model for Duchenne muscular dystrophy. Cell. 1997 Aug 22;90(4):729-38. (Medline PMID: 9288752)
  • Grady RM, Merlie JP, Sanes JR. Subtle neuromuscular defects in utrophin-deficient mice. J Cell Biol. 1997 Feb 24;136(4):871-82. (Medline PMID: 9049252)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
011196-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,545.65
Non-Profit / For-Profit
Aliquot Approximate quantity2 : 20-40 embryos / aliquot
011196-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $869.88
Non-Profit / For-Profit
Aliquot Approximate quantity3
011196-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.