Strain Detail Sheet

Strain Name    :

B6.129-Utrntm1Jrs/Mmucd

Stock Number :

011196-UCD

Other Names   :

Utrophin, B6.129-Utrntm1Jrs/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Utrntm1Jrs
Name: targeted mutation 1, Joshua R Sanes
Alteration at locus: Knockout
Gene Symbol: Utrn
Name: utrophin
Chromosome: 10
Alteration at locus: Knockout

Genetic Alterations:
a neo cassette was inserted into the utrophin exon that encodes the beginning of the cystein-rich domain

Genotype Determination:

ES Cell Line: R1 derived from C57BL/6 x 129

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Utrophin null mice are normal in appearance and behavior and show no obvious defects in muscle or non-muscle tissue.

Heterozygous phenotype: Heterozygotes do not show any known morphological abnormalities.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Utrntm1Jrs/Utrntm1Jrs
        involves: 129S1/Sv * 129X1/SvJ
  • nervous system phenotype
    • abnormal neuromuscular synapse morphology (MGI Ref ID J:39218)
      • density of acetylcholine receptors in the synapse is 30% lower than in controls, however do not exhibit any behavior abnormalities
      • the number of junctional folds is reduced by 50% in skeletal muscle by P11 and persists into adulthood, however nerve terminals and Schwann cells appear normal
Utrntm1Jrs/Utrntm1Jrs
        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • mortality/aging
    • increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:89397)
      • homozygotes are more sensitive to the excitoxic effects of kainate injections, with a higher mortality and more extensive development of hippocampal sclerosis, however susceptibility to kainate induced seizures is similar to that of wild-type
  • nervous system phenotype
    • abnormal nervous system physiology (MGI Ref ID J:89397)
      • homozygotes are more sensitive to the excitoxic effects of kainate injections, with a higher mortality and more extensive development of hippocampal sclerosis, however susceptibility to kainate induced seizures is similar to that of wild-type
  • homeostasis/metabolism phenotype
    • increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:89397)
      • homozygotes are more sensitive to the excitoxic effects of kainate injections, with a higher mortality and more extensive development of hippocampal sclerosis, howev er susceptibility to kainate induced seizures is similar to that of wild-type

Strain of Origin: C57BL/6 / 129

Strain genetic background: C57BL/6

Strain Development: Chimeric mice were bred to C57BL/6 and mice heterozygous for the mutant allele were selected by genotyping.

These heterozygotes were bred together to produce homozygotes. Repeated backcrosses to C57BL/6 were made by breeding the homozygous null mouse with a C57BL/6 mouse. The heterozygous mice generated from this breeding scheme were bred together to produce homozyotes. This breeding scheme was repeated 6 times in order to increase the purity ot the C57BL/6 background.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Developmental Biology
  • Models for Human Disease
  • Musculoskeletal

Strain Origin

Donor: Joshua Sanes, Ph.D., Harvard University

Primary Reference:

  • Grady RM, Teng H, Nichol MC, Cunningham JC, Wilkinson RS, Sanes JR. Skeletal and cardiac myopathies in mice lacking utrophin and dystrophin: a model for Duchenne muscular dystrophy. Cell. 1997 Aug 22;90(4):729-38. (Medline PMID: 9288752)
  • Grady RM, Merlie JP, Sanes JR. Subtle neuromuscular defects in utrophin-deficient mice. J Cell Biol. 1997 Feb 24;136(4):871-82. (Medline PMID: 9049252)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
011196-UCD-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
011196-UCD-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
011196-UCD-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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