Allele Symbol: Utrntm1Jrs
Name: targeted mutation 1, Joshua R Sanes
Alteration at locus: Knockout
Gene Symbol: Utrn
Alteration at locus: Knockout
ES Cell Line: R1 derived from C57BL/6 x 129
Homozygous phenotype: Utrophin null mice are normal in appearance and behavior and show no obvious defects in
muscle or non-muscle tissue.
Heterozygous phenotype: Heterozygotes do not show any known morphological abnormalities.
Strain of Origin: C57BL/6 / 129
Strain genetic background: C57BL/6
Strain Development: Chimeric mice were bred to C57BL/6 and mice heterozygous for the mutant allele were selected by
These heterozygotes were bred together to produce homozygotes. Repeated backcrosses to C57BL/6 were made by breeding the homozygous null mouse with a C57BL/6 mouse. The heterozygous mice generated from this breeding scheme were bred together to produce homozyotes. This breeding scheme was repeated 6 times in order to increase the purity ot the C57BL/6 background.
Suggested Control Mice:
Donor: Joshua Sanes, Ph.D., Harvard University
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.