Strain Detail Sheet

Strain Name    :

STOCK Fgf8tm1.1Mrt/Mmucd

Stock Number :

011201-UCD

Other Names   :

Fgf8 neo, STOCK Fgf8tm1.1Mrt/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Fgf8tm1.1Mrt
Name: targeted mutation 1.1, Gail R Martin
Alteration at locus: Targeted Mutation
Gene Symbol: Fgf8
Name: fibroblast growth factor 8
Chromosome: 19
Alteration at locus: Targeted Mutation

Genetic Alterations:
A neo cassette flanked by frt sites and followed 3' by a loxP site was inserted into the intron upstream of exon 2, and a second loxP site was inserted 3' of the stop codon in exon 3. In this allele, the two loxP sites flank conserved coding sequences of the Fgf8 gene. In this description, the exon designation conforms with that used in published articles, however, it should be noted that "exon 1" in the Fgf8 locus consists of 4 small exons, such that according to the official annotation of the Fgf8 locus, coding sequences in exons 5 and 6 are flanked by loxP sites in the Fgf8 neo allele.

Genotype Determination:

ES Cell Line: HM-1 derived from 129 x C57BL/6 hybrid

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Fgf8 neo homozygotes die perinatally with defects in multiple organs, including brain, craniofacial and kidney.

Heterozygous phenotype: Fgf8 neo heterozygotes are viable and fertile and appear to be normal.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Fgf8tm1.1Mrt/Fgf8tm1.1Mrt
        involves: 129P2/OlaHsd
  • mortality/aging
    • complete neonatal lethality (MGI Ref ID J:45909)
      • all homozygotes die within a day of birth
  • nervous system phenotype
    • abnormal midbrain morphology (MGI Ref ID J:45909)
      • inferior collicular (posterior midbrain) tissue is deleted
    • absent cerebellum (MGI Ref ID J:45909)
      • cerebellar tissue (anterior hindbrain) is absent
    • absent olfactory bulb (MGI Ref ID J:45909)
      • olfactory bulbs are absent

Strain of Origin: 129 x C57BL/6

Strain genetic background: mixed, including 129, CD1, C57BL/6, FVB/N, Swiss Black

Strain Development: This mouse line was bred to various mouse strains leading to a mixed genetic background.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Developmental Biology
  • Neurobiology

Strain Origin

Donor: Gail R. Martin, University of California School of Medicine

Primary Reference:

  • Meyers EN, Lewandoski M, Martin GR. An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination. Nat Genet. 1998 Feb;18(2):136-41. (Medline PMID: 9462741)
  • Storm EE, Rubenstein JL, Martin GR. Dosage of Fgf8 determines whether cell survival is positively or negatively regulated in the developing forebrain. Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1757-62. Epub 2003 Feb 6. (Medline PMID: 12574514 )
  • Meyers EN, Martin GR. Differences in left-right axis pathways in mouse and chick: functions of FGF8 and SHH. Science. 1999 Jul 16;285(5426):403-6. (Medline PMID: 10411502)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
011201-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
011201-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
011201-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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