Strain Name:
STOCK Fgf8tm1.3Mrt/Mmucd
Stock Number:
011202-UCD
Citation ID:
RRID:MMRRC_011202-UCD
Other Names:
Fgf8 flox, STOCK Fgf8tm1.3Mrt/Mmcd

Strain Information

Fgf8tm1.3Mrt
Name: fibroblast growth factor 8; targeted mutation 1.3, Gail R Martin
Synonyms: Fgf8clc, Fgf8flox, Fgf8fl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Targeted Mutation
Fgf8
Name: fibroblast growth factor 8
Synonyms: Fgf-8, Aigf
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Targeted Mutation
NCBI: 14179
HGNC: HGNC:3686
Homologene: 7715
Genetic Alterations
Fgf8 flox was derived from Fgf8 neo (described below) by mating Fgf8 neo/+ mice with mice carrying a beta-actin-flp transgene. In offspring that inherited both Fgf8 neo and the flp transgene, the neo cassette in the Fgf8 neo allele was excised via flp-mediated recombination, resulting in Fgf8 flox: a frt site and a loxP site inserted in the intron upstream of exon 2, and a loxP site inserted 3' of the stop codon in exon 3. The beta-actin-flp transgene was removed from the Fgf8 flox line by mating to wild type mice and selecting for animals carrying Fgf8 flox but not beta-actin-flp.

Fgf8 neo was produced by inserting a neo cassette flanked by frt sites and followed 3' by a loxP site into the intron upstream of exon 2, and a second loxP site was inserted 3' of the stop codon in exon 3. In this allele, the two loxP sites flank conserved coding sequences of the Fgf8 gene. In this description, the exon designation conforms with that used in published articles, however, it should be noted that "exon 1" in the Fgf8 locus consists of 4 small exons, such that according to the official annotation of the Fgf8 locus, coding sequences in exons 5 and 6 are flanked by loxP sites in the Fgf8 neo allele.
ES Cell Line
HM-1 derived from 129P2/OlaHsd-Hprtb-m3
Phenotype
Homozygous: Fgf8 flox homozygotes are viable and fertile and do not display any obvious phenotypes.

Heterozygous:
Fgf8 flox heterozygotes are viable and fertile and do not display any obvious phenotypes.
Mammalian Phenotype Terms
Allelic Composition: Fgf8tm1.3Mrt/Fgf8tm1.3Mrt (Genetic Background: involves: 129P2/OlaHsd )

Allelic Composition: Fgf8tm1.2Mrt/Fgf8tm1.3Mrt (Genetic Background: involves: 129P2/OlaHsd )

MeSH Terms
  • Actins/genetics
  • Alleles
  • Animals
  • Congenital Abnormalities/embryology
  • Congenital Abnormalities/genetics
  • Crosses, Genetic
  • DNA Nucleotidyltransferases/metabolism
  • Embryonic and Fetal Development
  • Fibroblast Growth Factor 8
  • Fibroblast Growth Factors
  • Gene Library
  • Growth Substances/biosynthesis
  • Growth Substances/genetics
  • Humans
  • Integrases/metabolism
  • Mice
  • Mice, Transgenic
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic
  • Recombination, Genetic
  • Viral Proteins
  • Branchial Region/physiology
  • Cell Survival
  • Fibroblast Growth Factors/physiology
  • Gene Expression Regulation, Developmental
  • Gene Targeting
  • Incisor/embryology
  • Integrases/deficiency
  • Integrases/genetics
  • Integrases/physiology
  • Jaw Abnormalities/embryology
  • Jaw Abnormalities/genetics
  • Mesoderm/cytology
  • Mice, Knockout
  • Morphogenesis/genetics
  • Regulatory Sequences, Nucleic Acid
  • Tooth Abnormalities/embryology
  • Tooth Abnormalities/genetics
  • Ectoderm/metabolism
  • Extremities/embryology
  • Female
  • Fibroblast Growth Factor 4
  • Fibroblast Growth Factors/genetics
  • Hedgehog Proteins
  • In Situ Hybridization
  • Limb Deformities, Congenital/genetics
  • Male
  • Mice, Mutant Strains
  • Pregnancy
  • Proteins/genetics
  • Proteins/physiology
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/physiology
  • Signal Transduction
  • Trans-Activators
Strain Development
This mouse line was bred to various mouse strains (see above), leading to a mixed genetic background.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Apoptosis
  • Developmental Biology
  • Neurobiology
Donor
Gail R. Martin, Ph.D., University of California, San Francisco
Primary Reference
  • Meyers EN, Lewandoski M, Martin GR. An Fgf8 mutant allelic series generated by Cre- and Flp-mediated recombination. Nat Genet. 1998 Feb;18(2):136-41. (Medline PMID: 9462741)
  • Trumpp A, Depew MJ, Rubenstein JL, Bishop JM, Martin GR. Cre-mediated gene inactivation demonstrates that FGF8 is required for cell survival and patterning of the first branchial arch. Genes Dev. 1999 Dec 1;13(23):3136-48. (Medline PMID: 10601039)
  • Lewandoski M, Sun X, Martin GR. Fgf8 signalling from the AER is essential for normal limb development. Nat Genet. 2000 Dec;26(4):460-3. (Medline PMID: 11101846)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
011202-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
011202-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
011202-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.