Strain Name:
STOCK Fgf4tm1.3Mrt/Mmucd
Stock Number:
011205-UCD
Citation ID:
RRID:MMRRC_011205-UCD
Other Names:
Fgf4 delta2,3, STOCK Fgf4tm1.3Mrt/Mmcd

Strain Information

Fgf4tm1.3Mrt
Name: fibroblast growth factor 4; targeted mutation 1.3, Gail R Martin
Synonyms: Fgf4delta2,3
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Targeted Mutation
Fgf4
Name: fibroblast growth factor 4
Synonyms: kFGF, Fgfk, Hstf-1, Fgf-4, Hst1
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Targeted Mutation
NCBI: 14175
HGNC: HGNC:3682
Homologene: 1522
Genetic Alterations
A cassette consisting of a Pgk-neo gene flanked by frt sites, and a single loxP site was inserted into intron 1, and a second loxP site was inserted 3' of the stop codon in exon 3, thereby generating an Fgf4 neo allele. Using a beta-actin-flp transgene, neo was excised from the Fgf4 neo allele to generate Fgf4 flox, in which essential coding sequences in exons 2 and 3 are flanked by loxP sites. Using a beta-actin-cre transgene, the loxP-flanked sequences were excised from the Fgf4 flox allele to generate Fgf4 delta2,3.
Genotype Determination
ES Cell Line
CGR8 derived from 129P2/OlaHsd
Phenotype
Homozygous: Fgf4 delta2,3 homozygote embryos fail to differentiate properly at embryonic day 4.5, leading to postimplantation lethality.

Heterozygous:
Fgf4 delta2,3 heterozygotes are viable and fertile and do not diplay any obvious phenotypes.
MeSH Terms
  • Animals
  • DNA-Binding Proteins/genetics
  • Ectoderm/metabolism
  • Ectoderm/physiology
  • Egg Proteins/genetics
  • Feedback/physiology
  • Fibroblast Growth Factor 4
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism
  • Gene Expression Regulation, Developmental
  • Genes, Lethal
  • Hedgehog Proteins
  • Homeodomain Proteins
  • Integrases/genetics
  • Limb Buds/embryology
  • Membrane Glycoproteins/genetics
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Proteins/genetics
  • Proto-Oncogene Proteins/genetics
  • Proto-Oncogene Proteins/metabolism
  • Receptors, Cell Surface
  • Signal Transduction/genetics
  • Trans-Activators
  • Viral Proteins
  • Zona Pellucida/physiology
  • Zona Pellucida Glycoproteins
Strain Development
This mouse line was bred to various mouse strains leading to a mixed genetic background.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Developmental Biology
Donor
Gail R. Martin, Ph.D., University of California, San Francisco
Primary Reference
Sun X, Lewandoski M, Meyers EN, Liu YH, Maxson RE Jr, Martin GR. Conditional inactivation of Fgf4 reveals complexity of signalling during limb bud development. Nat Genet. 2000 May;25(1):83-6. (Medline PMID: 10802662)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
011205-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
011205-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
011205-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.