Strain Detail Sheet

Strain Name    :

129S6;B6-Skc2/Mmucd

Stock Number :

000113-UCD

Other Names   :

Pete Rose hair, Skcm02Jus, 129S6;B6-Skc2/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Skc2
Name: skin/coat color 2
Chromosome: 11; 50.5 cM
Alteration at locus: Chemically Induced
Gene Symbol: Skc2
Name: skin/coat color 2
Chromosome: 11
Alteration at locus: Chemically Induced

Genetic Alterations:
Offspring of an ENU-Induced male

Genotype Determination:

    ES Cell Line: Not Applicable

    Strain Description [Including phenotype, strain background, strain development and suggested control mice]

    Phenotype

    Hair appears to be more coarse. At weaning hair is thinner around the genitalia and inner back thighs. Hairloss progresses as the animals age to a point where there is barely any hair remaining. (aprox. 2-3 months).


    Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
          assigned by genotype

    The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
    Skc2/Skc2+
            Background Not Specified
    • integument phenotype
      • disheveled coat (MGI Ref ID J:89098)
        • scruffy coat with hair sticking straight out

    Strain of Origin: C57BL/6

    Strain genetic background: 129 SvEv/Tac

    Strain Development: For strain development, view: http://www.mouse-genome.bcm.tmc.edu/ENU/ENUexperiment.asp#Balancer

    Suggested Control Mice:

    Wild-type littermates

    Research Applications

      Strain Origin

      Donor:  

      Primary Reference:

      Kile BT, Hentges KE, Clark AT, Nakamura H, Salinger AP, Liu B, Box N, Stockton DW, Johnson RL, Behringer RR, Bradley A, Justice MJ. Functional genetic analysis of mouse chromosome 11. Nature. 2003 Sep 4;425(6953):81-6. (Medline PMID: 12955145)

      Colony and Husbandry Information

      Special Considerations

      Phenotype is easier to see at 4-5 weeks, so it is recommended that the entire litters be saved. And are monitored 1-2 weeks after weaning to be certain of affected versus unaffected status. Additionally these animals are more prone to eye infections/skin problems, so a topical treatment with antibiotics may be necessary.

      Health Status Report

      Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

      Appearance

      Coat color: Agouti

      Other:

      Breeding

      MMRRC Breeding System: Out breeding.
      Please note: The MMRRC has always maintained this strain in a cryopreserved format. Information in the "Breeding" and "Reproductive statistics" sections has been provided by the Donating Investigator

      Breeding Scheme(s):

      • Homozygous female x Heterozygous male
      • Dominant mutation, maitained as a heterozygote.
      • View Mating Scheme

      Generation: Unknown

      Overall Breeding Performance: Good

      Reproductive Statistics

      Viability and Fertility:FemaleMale
      Homozygotes are viable: Unknown Unknown
      Homozygotes are fertile: Unknown Unknown
      Heterozygotes are fertile: Yes Yes
       
      Age Reproductive Decline: Unknown Unknown

      Average litter size: 8

      Recommended wean age: 3 weeks

      Order Request Information

      Availability Level

      Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

      Conditions of Distribution [Including applicable technology transfer agreements]

      Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

      The donor or their institution limits the distribution to non-profit institutions only.

      Fees

      Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

      Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
      MMRRC Item # - Description Distribution
      Fee/unit (US $)
      Units Notes
      000113-UCD-RESUSLitter recovered from cryo-archive
      $2,022.00
      Non-Profit
      Litter Recovered litter1; additional fees for any special requests.
      000113-UCD-SPERMCryo-preserved spermatozoa
      $437.00
      Non-Profit
      Aliquot Approximate quantity.2
      000113-UCD-EMBRYOCryo-preserved embryos
      $1,038.00
      Non-Profit
      Aliquot Approximate quantity3: 20-40 embryos / aliquot

      1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

      2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

      3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

      To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



      To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



      The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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      Generated: 04/18/2014