Strain Name    : B6;129S2-Mycntm1Par/Mmnc

Stock Number : 011389-UNC

Gene Information

(provided by MGI)
Allele Symbol: Mycntm1Par
Name: targeted mutation 1, Luis Parada
Chromosome: 12
Alteration at locus: Knockout
Gene Symbol: Mycn
Name: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Chromosome: 12
Alteration at locus: Knockout
Genetic Alterations:
Neo cassette was recombined as an Nmy neo fusion into the firs Nmyc exon.

Genotype Determination:

  • The genotyping protocol for this strain is currently not available. The MMRRC will develop a protocol if this strain is ordered.

ES Cell Line: D3 derived from 129S2/SvPas

Phenotype

Homozygous phenotype: Mice die at E11.5- E12.5 They have defective epithelia notably in the lung, kidney and CNS.

Heterozygous phenotype: No observanble phenotype.

Mammalian Phenotype Terms:
Allelic Composition: Mycntm1Par/Mycntm1Par (Genetic Background: involves: 129S2/SvPas * C57BL/6J )

Allelic Composition: Mycntm1Par/Mycn+ (Genetic Background: involves: 129S2/SvPas * C57BL/6J )

Allelic Composition: Mycntm1Jrt/Mycntm1Par (Genetic Background: involves: 129S2/SvPas * C57BL/6J )

Strain of Origin: 129

Strain genetic background: 129/C57Bl Hybrid

Strain Development: Interbreeding for 15 years

Suggested Control Mice:

  • Wildtype littermates

  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology

Donor: Luis F. Parada, Ph.D., UT Southwestern Medical Center

Primary Reference:

  • Stanton BR, Reid SW, Parada LF. Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells. Mol Cell Biol. 1990 Dec;10(12):6755-8. (Medline PMID: 1701023)
  • Stanton BR, Perkins AS, Tessarollo L, Sassoon DA, Parada LF. Loss of N-myc function results in embryonic lethality and failure of the epithelial component of the embryo to develop. Genes Dev. 1992 Dec;6(12A):2235-47. (Medline PMID: 1459449)
  • Bates CM, Kharzai S, Erwin T, Rossant J, Parada LF. Role of N-myc in the developing mouse kidney. Dev Biol. 2000 Jun 15;222(2):317-25. (Medline PMID: 10837121)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
011389-UNC-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.