Strain Detail Sheet

Strain Name    :

B6;129S2-Mycntm1Par/Mmnc

Stock Number :

011389-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Mycntm1Par
Name: targeted mutation 1, Luis Parada
Alteration at locus: Knockout
Gene Symbol: Mycn
Name: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
Chromosome: 12
Alteration at locus: Knockout

Genetic Alterations:
Neo cassette was recombined as an Nmy neo fusion into the firs Nmyc exon.

Genotype Determination:

  • The genotyping protocol for this strain is currently not available. The MMRRC will develop a protocol if this strain is ordered.

ES Cell Line: D3 derived from 129S2/SvPas

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Mice die at E11.5- E12.5 They have defective epithelia notably in the lung, kidney and CNS.

Heterozygous phenotype: No observanble phenotype.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Mycntm1Par/Mycn+
        involves: 129S2/SvPas * C57BL/6J
  • mortality/aging
    • partial postnatal lethality (MGI Ref ID J:3432)
      • heterozygotes are present at the expected Mendelian ratio at E18.5 but are significantly under-represented at 3 weeks
      • surviving heterozygotes are fertile, healthy and exhibit no differences in size, weight, or lifespan relative to wild-type mice
Mycntm1Par/Mycntm1Par
        involves: 129S2/SvPas * C57BL/6J
  • mortality/aging
    • complete embryonic lethality during organogenesis (MGI Ref ID J:3432)
      • homozygotes die at ~E11.5
      • by E12.5, homozygotes appear highly necrotic and pale (presumably due to anemia) and show no signs of life
  • nervous system phenotype
    • abnormal cranial ganglia morphology (MGI Ref ID J:3432)
      • by E11.5, cranial ganglia are generally reduced, with a mitotic rate of less than 25% of wild-type
      • small trigeminal ganglion (MGI Ref ID J:3432)
        • by E11.5, homozygotes display lack of organization and a reduction of neurite outgrowths in the trigeminal ganglia
    • abnormal embryonic neuroepithelial layer differentiation (MGI Ref ID J:3432)
      • at E9.5, homozygotes exhibit a wavy neuroepithelium and an aberrant ectodermal surface
      • by E11.5, the neuroepithelium is thin and lacks architectural complexity
    • abnormal forebrain development (MGI Ref ID J:3432)
      • at E9.5, homozygotes display an abnormal prosencephalon
    • abnormal medulla oblongata morphology (MGI Ref ID J:3432)
      • at E9.5, homozygotes display an abnormal roof plate in the myelencephalon
    • disorganized dorsal root ganglion (MGI Ref ID J:3432)
    • small dorsal root ganglion (MGI Ref ID J:3432)
      • by E11.5, homozygotes display a reduction of neurite outgrowths in the dorsal root ganglia
    • telencephalon hypoplasia (MGI Ref ID J:3432)
      • at E11.5, homozygotes display hypoplastic telencephalic structures
  • respiratory system phenotype
    • abnormal lung development (MGI Ref ID J:3432)
      • at E10.5, the mutant lung epithelium fails to undergo proper branching; as a result, mutant lungs appear as simple tube-like structures
      • however, no morphologic abnormalities are noted in the mesenchymal component
      • impaired branching involved in bronchus morphogenesis (MGI Ref ID J:3432)
        • at E10.5, mutant lungs fail to display branching morphogenesis
    • pulmonary hypoplasia (MGI Ref ID J:3432)
  • digestive/alimentary phenotype
    • abnormal intestinal epithelium morphology (MGI Ref ID J:3432)
      • at E10.5-E11.5, homozygotes display abnormal growth and/or disorganization of the gut epithelium
    • abnormal large intestine morphology (MGI Ref ID J:3432)
      • at E11.5, homozygotes lack an identifiable large intestine
    • abnormal stomach morphology (MGI Ref ID J:3432)
      • at E11.5, homozygotes lack an identifiable stomach
  • embryogenesis phenotype
    • abnormal embryogenesis/ development (MGI Ref ID J:3432)
      • as early as E9.5, homozygotes display hypoplasia of several organ systems, particularly those of epithelial origin such as the lung and gut
      • abnormal embryonic neuroepithelial layer differentiation (MGI Ref ID J:3432)
        • at E9.5, homozygotes exhibit a wavy neuroepithelium and an aberrant ectodermal surface
        • by E11.5, the neuroepithelium is thin and lacks architectural complexity
      • abnormal mesonephros morphology (MGI Ref ID J:3432)
        • at E9.5, some (but not all) homozygotes exhibit a slight delay in the initial formation of the mesonephric tubules
        • by E11.5, some tubules appear normal while others fail to form or undergo premature degeneration; the mesenchyme surrounding the mesonephric tubules is hypoplastic
        • abnormal Wolffian duct morphology (MGI Ref ID J:3432)
          • at E9.5, some (but not all) homozygotes exhibit a slight delay in the initial formation of the mesonephric duct
      • branchial arch hypoplasia (MGI Ref ID J:3432)
        • at E9.5, homozygotes display hypoplasia of the mandibular arch, the first postoral arch in the branchial arch series
      • decreased embryo size (MGI Ref ID J:3432)
        • at E9.5, homozygotes appear thinner in transverse dimension relative to wild-type embryos
      • embryonic growth arrest (MGI Ref ID J:3432)
        • homozygotes become growth arrested at ~E11.5
      • embryonic growth retardation (MGI Ref ID J:3432)
        • starting at E10.5, homozygotes exhibit progressive growth retardation
        • by E11.5, homozygotes are ~40% the size of wild-type embryos
  • cardiovascular system phenotype
    • abnormal dorsal aorta morphology (MGI Ref ID J:3432)
      • dilated dorsal aorta (MGI Ref ID J:3432)
        • at E11.5, the mutant dorsal aorta is dilated
    • hemorrhage (MGI Ref ID J:3432)
      • at E11.5, homozygotes are fragile and bleed easily, despite the presence of a beating heart
    • thin myocardium (MGI Ref ID J:3432)
      • at E11.5, homozygotes display a thinned myocardium
  • reproductive system phenotype
    • abnormal reproductive system development (MGI Ref ID J:3432)
      • at E11.5, homozygotes exhibit a hypoplastic genital ridge with an irregular, hobnail-like coelomic epithelium
      • both the stromal and germ cell components of the genital ridge are severly hypoplastic (~1/10 of wild-type size)
      • abnormal Wolffian duct morphology (MGI Ref ID J:3432)
        • at E9.5, some (but not all) homozygotes exhibit a slight delay in the initial formation of the mesonephric duct
  • craniofacial phenotype
    • branchial arch hypoplasia (MGI Ref ID J:3432)
      • at E9.5, homozygotes display hypoplasia of the mandibular arch, the first postoral arch in the branchial arch series
  • growth/size phenotype
    • decreased embryo size (MGI Ref ID J:3432)
      • at E9.5, homozygotes appear thinner in transverse dimension relative to wild-type embryos
    • embryonic growth retardation (MGI Ref ID J:3432)
      • starting at E10.5, homozygotes exhibit progressive growth retardation
      • by E11.5, homozygotes are ~40% the size of wild-type embryos
  • hematopoietic system phenotype
    • anemia (MGI Ref ID J:3432)
      • by E12, homozygotes appear severely anemic

Strain of Origin: 129

Strain genetic background: 129/C57Bl Hybrid

Strain Development: Interbreeding for 15 years

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology

Strain Origin

Donor: Luis F. Parada, Ph.D., UT Southwestern Medical Center

Primary Reference:

  • Stanton BR, Reid SW, Parada LF. Germ line transmission of an inactive N-myc allele generated by homologous recombination in mouse embryonic stem cells. Mol Cell Biol. 1990 Dec;10(12):6755-8. (Medline PMID: 1701023)
  • Stanton BR, Perkins AS, Tessarollo L, Sassoon DA, Parada LF. Loss of N-myc function results in embryonic lethality and failure of the epithelial component of the embryo to develop. Genes Dev. 1992 Dec;6(12A):2235-47. (Medline PMID: 1459449)
  • Bates CM, Kharzai S, Erwin T, Rossant J, Parada LF. Role of N-myc in the developing mouse kidney. Dev Biol. 2000 Jun 15;222(2):317-25. (Medline PMID: 10837121)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
011389-UNC-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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