Allele Symbol: Apoa5tm1Hgc
Name: targeted mutation 1, Edward M Rubin
Alteration at locus: Knockout
Gene Symbol: Apoa5
Name: apolipoprotein A-V
Alteration at locus: Knockout
ES Cell Line: Not specified derived from 129X1/SvJ
Homozygous: Homozygous Apoav knockout mice (-/-)have about four times as much plasma triglyceride as their wild-type littermates. Characterization of lipoprotein particles by fast-protein liquid chromatography (FPLC) and gradient-gel electrophoresis (GGE) reveal that levels of very low density lipoprotein (VLDL) particles are increased in the homozygous knockout mice.
Heterozygous: Heterozygous Apoav knockout mice (+/-)show elevated levels of plasma triglycerides. VLDL levels in a heterozygous knockout mouse were intermediate between the homozygous knockout and control mouse.
Strain of Origin: 129SvJ
Strain genetic background: FVB.129X1(B6)
Strain Development: Targeted clones (129/SvJ) were injected into C57BL/6 blastocysts and chimeric males were bred to C57BL/6J females. Agouti offspring were tested for germ line transmission of the targeted allele by PCR and heterozygous animals were intercrossed to obtain homozygous deletion.
Suggested Control Mice: Wild-type littermates
Donor: Edward M. Rubin, Ph.D., Lawrence Berkeley National Laboratory
Pennacchio LA, Olivier M, Hubacek JA, Cohen JC, Cox DR, Fruchart JC, Krauss RM, Rubin EM. An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing. Science. 2001 Oct 5;294(5540):169-73. (Medline PMID: 11588264 )
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
|Aliquot||Approximate quantity3: 20-40 embryos / aliquot|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.