Strain Detail Sheet

Strain Name    :

FVB.129X1(B6)-Apoa5tm1Hgc/Mmucd

Stock Number :

011467-UCD

Other Names   :

Apoav knockout, FVB.129X1(B6)-Apoa5tm1Hgc/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Apoa5tm1Hgc
Name: targeted mutation 1, Edward M Rubin
Alteration at locus: Knockout
Gene Symbol: Apoa5
Name: apolipoprotein A-V
Chromosome: 9
Alteration at locus: Knockout

Genetic Alterations:
AV gene was targeted by inserting a neomycin cassette between the Xho1 and EcoRI restriction sites in the AV gene. The neomycin cassette replaces the three exons predicted to encode Apoav.

Genotype Determination:

ES Cell Line: Not specified derived from 129X1/SvJ

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous: Homozygous Apoav knockout mice (-/-)have about four times as much plasma triglyceride as their wild-type littermates. Characterization of lipoprotein particles by fast-protein liquid chromatography (FPLC) and gradient-gel electrophoresis (GGE) reveal that levels of very low density lipoprotein (VLDL) particles are increased in the homozygous knockout mice.

Heterozygous: Heterozygous Apoav knockout mice (+/-)show elevated levels of plasma triglycerides. VLDL levels in a heterozygous knockout mouse were intermediate between the homozygous knockout and control mouse.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Apoa5tm1Hgc/Apoa5+
        involves: 129X1/SvJ * C57BL/6
  • homeostasis/metabolism phenotype
    • increased circulating VLDL cholesterol level (MGI Ref ID J:72092)
      • VLDL levels are intermediate between homozygotes and wild-type controls
Apoa5tm1Hgc/Apoa5tm1Hgc
        involves: 129X1/SvJ * C57BL/6
  • homeostasis/metabolism phenotype
    • increased circulating VLDL cholesterol level (MGI Ref ID J:72092)
    • increased circulating triglyceride level (MGI Ref ID J:72092)
      • 4x as much plasma triglyceride as wild-type

Strain of Origin: 129SvJ

Strain genetic background: FVB.129X1(B6)

Strain Development: Targeted clones (129/SvJ) were injected into C57BL/6 blastocysts and chimeric males were bred to C57BL/6J females. Agouti offspring were tested for germ line transmission of the targeted allele by PCR and heterozygous animals were intercrossed to obtain homozygous deletion.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cardiovascular
  • Models for Human Disease
  • Research Tools

Strain Origin

Donor: Edward M. Rubin, Ph.D., Lawrence Berkeley National Laboratory

Primary Reference:

Pennacchio LA, Olivier M, Hubacek JA, Cohen JC, Cox DR, Fruchart JC, Krauss RM, Rubin EM. An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing. Science. 2001 Oct 5;294(5540):169-73. (Medline PMID: 11588264 )

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
011467-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
011467-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
011467-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/18/2014