Strain Name:
C.129-Cd40tm1Kik Cd86tm2Shr Cd80tm1Shr/Mmnc
Stock Number:
011489-UNC
Citation ID:
RRID:MMRRC_011489-UNC
Other Names:
B7-1, B7-2

Gene Information

Cd80tm1Shr
Name: CD80 antigen; targeted mutation 1, Arlene H Sharpe
Synonyms: B7.1, B7-1-, CD80-, B7-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
Cd86tm1Shr
Name: CD86 antigen; targeted mutation 1, Arlene H Sharpe
Synonyms: B7.2 KO, B7-2-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
Cd40tm1Kik
Name: CD40 antigen; targeted mutation 1, Hitoshi Kikutani
Synonyms: CD40 Ko, Tnfrsf5tm1Kik, Tnfrsf5-, CD40-, CD40KO
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knockout
Cd80
Name: CD80 antigen
Synonyms: B7-1, Ly53, Ly-53, Cd28l, B7.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
NCBI: 12519
VEGA: 16
HGNC: HGNC:1700
Homologene: 3804
Cd86
Name: CD86 antigen
Synonyms: B7-2, B7.2, MB7-2, B70, Ly-58, Cd28l2, Ly58
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
NCBI: 12524
HGNC: HGNC:1705
Homologene: 10443
Cd40
Name: CD40 antigen
Synonyms: Tnfrsf5, Cd40, Bp50, p50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knockout
NCBI: 21939
Homologene: 954
Genetic Alterations
Neo cassettes were inserted into the CD80/86 locus, deleting that, and then separately into the CD40 locus
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    J1 derived from 129S4/SvJae
    Phenotype
    Homozygous phenotype: Mice have dramatically impaired ability to present Ags to T cells and thus have markedly reduced immune responses

    Heterozygous: None
    Mammalian Phenotype Terms
    Allelic Composition: Cd80tm1Shr/Cd80tm1Shr (Genetic Background: involves: 129S4/SvJae )

    Allelic Composition: Cd86tm1Shr/Cd86tm1Shr (Genetic Background: B6.129S4-Cd86tm1Shr )

    Allelic Composition: Cd86tm1Shr/Cd86tm1Shr (Genetic Background: involves: 129S4/SvJae )

    Allelic Composition: Cd86tm1Shr/Cd86tm1Shr (Genetic Background: NOD.129S4-Cd86tm1Shr )

    Allelic Composition: Cd40tm1Kik/Cd40tm1Kik (Genetic Background: B6.129P2-Cd40tm1Kik/J )

    Allelic Composition: Cd40tm1Kik/Cd40tm1Kik (Genetic Background: involves: 129P2/OlaHsd )

    Allelic Composition: Cd40tm1Kik/Cd40tm1Kik (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

    MeSH Terms
    • Abatacept
    • Adjuvants, Immunologic/chemistry
    • Animals
    • Antigens/immunology
    • Antigens, CD/biosynthesis
    • Antigens, CD/physiology
    • Antigens, Differentiation/biosynthesis
    • Antigens, T-Independent/immunology
    • B7-1 Antigen/biosynthesis
    • B7-1 Antigen/physiology
    • B7-2 Antigen
    • CTLA-4 Antigen
    • Freund's Adjuvant/chemistry
    • Freund's Adjuvant/immunology
    • Germinal Center/drug effects
    • Germinal Center/immunology
    • Germinal Center/metabolism
    • Haptens
    • Hemocyanins/immunology
    • Immunoconjugates
    • Immunoglobulin Class Switching/drug effects
    • Immunoglobulin Class Switching/immunology
    • Immunoglobulin Fc Fragments/biosynthesis
    • Membrane Glycoproteins/biosynthesis
    • Membrane Glycoproteins/physiology
    • Mice
    • Mice, Inbred BALB C
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Antibody Formation
    • Antigens, CD/genetics
    • Antigens, CD/metabolism
    • Antigens, Differentiation, B-Lymphocyte/genetics
    • Antigens, Differentiation, B-Lymphocyte/metabolism
    • Antigens, T-Independent/metabolism
    • B-Lymphocytes/immunology
    • Base Sequence
    • CD40 Antigens
    • DNA Primers/genetics
    • Female
    • Gene Targeting
    • Granulocytes
    • Humans
    • Immunoglobulin Class Switching
    • Immunoglobulin M/biosynthesis
    • Immunohistochemistry
    • Job Syndrome/immunology
    • Leukocyte Count
    • Lymphoid Tissue/immunology
    • Lymphoid Tissue/pathology
    • Male
    • Mice, Mutant Strains
    • Molecular Sequence Data
    • Phenotype
    • T-Lymphocytes/immunology
    • Antigen-Presenting Cells/physiology
    • Antigens, CD/immunology
    • B7-1 Antigen/immunology
    • B7-1 Antigen/metabolism
    • Bone Marrow Transplantation/adverse effects
    • CD4-Positive T-Lymphocytes/immunology
    • CD8-Positive T-Lymphocytes/immunology
    • Chronic Disease
    • Disease Models, Animal
    • Graft vs Host Disease/etiology
    • Graft vs Host Disease/immunology
    • Graft vs Host Disease/pathology
    • Intestinal Diseases/etiology
    • Intestinal Diseases/immunology
    • Membrane Glycoproteins/immunology
    • Membrane Glycoproteins/metabolism
    • Organ Specificity
    • Skin Diseases/etiology
    • Skin Diseases/immunology
    • Transplantation, Homologous
    Strain Development
    CD80/86 double KO made in a 129 ES cell and BC by Arlene Sharpe to BALB/c >5 times then transferred to the Shlomchik lab where strain was backcrossed. CD40 KO obtained from JAX on a BALB/c background (JAX strain number 002927), after >18 BC generations to BALB/c. Strains intercrossed to homozygosity by donor and maintained by sib mating since (date), approx. 2 generation
    Suggested Control Mice
    • Wildtype littermates
    • Apoptosis
    • Cancer
    • Dermatology
    • Immunology and Inflammation
    • Models for Human Disease
    Donor
    Mark Shlomchik, Ph.D., Yale University School of Medicine
    Primary Reference
    • Borriello F, Sethna MP, Boyd SD, Schweitzer AN, Tivol EA, Jacoby D, Strom TB, Simpson EM, Freeman GJ, Sharpe AH. B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation. Immunity. 1997 Mar;6(3):303-13. (Medline PMID: 9075931)
    • Kawabe T, Naka T, Yoshida K, Tanaka T, Fujiwara H, Suematsu S, Yoshida N, Kishimoto T, Kikutani H. The immune responses in CD40-deficient mice: impaired immunoglobulin class switching and germinal center formation. Immunity. 1994 Jun;1(3):167-78. (Medline PMID: 7534202)
    • Anderson BE, McNiff JM, Jain D, Blazar BR, Shlomchik WD, Shlomchik MJ. Distinct roles for donor- and host-derived antigen-presenting cells and costimulatory molecules in murine chronic graft-versus-host disease: requirements depend on target organ. Blood. 2005 Mar 1;105(5):2227-34. Epub 2004 Nov 2. (Medline PMID: 15522961)

    Colony and Husbandry Information

    Donor maintained on trimethoprim-sulfamethoxisole every other week as a precaution due to immunosuppression.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
    011489-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.