Strain Name:
B6;129-Btctm1Dcl/Mmnc
Stock Number:
011531-UNC
Citation ID:
RRID:MMRRC_011531-UNC
Other Names:
betacellulin 

Strain Information

Btctm1Dcl
Name: betacellulin, epidermal growth factor family member; targeted mutation 1, David C Lee
Synonyms: Btc-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Knockout
Btc
Name: betacellulin, epidermal growth factor family member
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Knockout
NCBI: 12223
HGNC: HGNC:1121
Homologene: 1309
Genetic Alterations
A neo cassette replaces a region including part of intron 2 and all of exon 3.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
    Phenotype
    Homozygous: No overt phenotype, viable and fertile

    Heterozygous: No overt phenotype, viable and fertile
    Mammalian Phenotype Terms
    Allelic Composition: Btctm1Dcl/Btctm1Dcl (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J )

    MeSH Terms
    • ADAM Proteins
    • ADAM17 Protein
    • Animals
    • Betacellulin
    • Bone Morphogenetic Proteins/physiology
    • Epidermal Growth Factor/deficiency
    • Epidermal Growth Factor/genetics
    • Epidermal Growth Factor/physiology
    • Gene Expression Regulation, Developmental
    • Heart Defects, Congenital/embryology
    • Heart Defects, Congenital/genetics
    • Heart Defects, Congenital/metabolism
    • Heart Valves/abnormalities
    • Heart Valves/embryology
    • Heart Valves/metabolism
    • Heparin-binding EGF-like Growth Factor
    • Intercellular Signaling Peptides and Proteins/deficiency
    • Intercellular Signaling Peptides and Proteins/genetics
    • Intercellular Signaling Peptides and Proteins/physiology
    • Lung/abnormalities
    • Metalloendopeptidases/deficiency
    • Metalloendopeptidases/genetics
    • Metalloendopeptidases/physiology
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Receptor, Epidermal Growth Factor/metabolism
    • Receptor, ErbB-4
    Strain Development
    Chimeras were mated to C57BL/6, and transmitting F1s were interbred or bred to C57BL/6. After 2-3 generations, strain was maintained by intercossing as random hybrid C57BL/6 x 129 for several years.
    Suggested Control Mice
    • Wildtype littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cardiovascular
    • Developmental Biology
    • Cell Biology
    • Diabetes/Obesity
    • Internal/Organ
    • Research tools
    • Immunology and Inflammation
    Donor
    David C. Lee, Ph.D., University of Georgia
    Primary Reference
    Jackson LF, Qiu TH, Sunnarborg SW, Chang A, Zhang C, Patterson C, Lee DC. Defective valvulogenesis in HB-EGF and TACE-null mice is associated with aberrant BMP signaling. EMBO J. 2003 Jun 2;22(11):2704-16. (Medline PMID: 12773386)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    011531-UNC-EMBRYO Cryo-preserved embryos $1,404.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    011531-UNC-SPERM Cryo-preserved spermatozoa $564.00 / Non-Profit Aliquot Approximate quantity3
    011531-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.