Strain Detail Sheet

Strain Name    :

B6;129-Aregtm1Dle/Mmnc

Stock Number :

011533-UNC

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Aregtm1Dle
Name: targeted mutation 1, David C Lee
Alteration at locus: Knockout
Gene Symbol: Areg
Name: amphiregulin
Chromosome: 5
Alteration at locus: Knockout

Genetic Alterations:
Exons 3 and 4 were replaced by a neo cassette.

Genotype Determination:

ES Cell Line: R1 derived from 129X1/SvJ x 129S1/Sv

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: Defective mammary gland ductal outgrowth. Young, uniparous females usually cannot lactate, older multiparous females often can lactate. Impaired response to partial hepatectomy.

Heterozygous phenotype: None


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Aregtm1Dle/Aregtm1Dle
        involves: 129P2/OlaHsd * C57BL/6
  • cardiovascular system phenotype
    • cardiovascular system phenotype (MGI Ref ID J:60750)
      • normal heart valves
  • endocrine/exocrine gland phenotype
    • abnormal branching of the mammary ductal tree (MGI Ref ID J:54980)
      • ducts in virgin pubescent glands hardly extended beyond the rudimentary anlage and only a few terminal end buds were observed at 6 weeks; at 12 weeks, ductal trees were underdeveloped and had persistent terminal end buds
    • abnormal lactation (MGI Ref ID J:54980)
      • mutant mammary glands could not sustain lactation and expression of milk protein genes was decreased; pups did not thrive due to decreased milk production of dams; however, lactation improved with aged mothers
    • abnormal mammary gland growth during pregnancy (MGI Ref ID J:54980)
      • during pregnancy, mammary glands exhibited defects in lobuloalveolar development; alveoli appear small and dense instead of engorged with fat droplets at day 18 of pregnancy
      • on day 2 of lactation, alveoli are compressed and overcrowded, and exhibit increased epithelial density with an irregular, compact alveolar morphology observed
  • reproductive system phenotype
    • abnormal branching of the mammary ductal tree (MGI Ref ID J:54980)
      • ducts in virgin pubescent glands hardly extended beyond the rudimentary anlage and only a few terminal end buds were observed at 6 weeks; at 12 weeks, ductal trees were underdeveloped and had persistent terminal end buds
    • abnormal lactation (MGI Ref ID J:54980)
      • mutant mammary glands could not su stain lactation and expression of milk protein genes was decreased; pups did not thrive due to decreased milk production of dams; however, lactation improved with aged mothers
    • abnormal mammary gland growth during pregnancy (MGI Ref ID J:54980)
      • during pregnancy, mammary glands exhibited defects in lobuloalveolar development; alveoli appear small and dense instead of engorged with fat droplets at day 18 of pregnancy
      • on day 2 of lactation, alveoli are compressed and overcrowded, and exhibit increased epithelial density with an irregular, compact alveolar morphology observed
  • integument phenotype
    • abnormal branching of the mammary ductal tree (MGI Ref ID J:54980)
      • ducts in virgin pubescent glands hardly extended beyond the rudimentary anlage and only a few terminal end buds were observed at 6 weeks; at 12 weeks, ductal trees were underdeveloped and had persistent terminal end buds
    • abnormal lactation (MGI Ref ID J:54980)
      • mutant mammary glands could not sustain lactation and expression of milk protein genes was decreased; pups did not thrive due to decreased milk production of dams; however, lactation improved with aged mothers
    • abnormal mammary gland growth during pregnancy (MGI Ref ID J:54980)
      • during pregnancy, mammary glands exhibited defects in lobuloalveolar development; alveoli appear small and dense instead of engorged with fat droplets at day 18 of pregnancy
      • on day 2 of lactation, alveoli are compressed and overcrowded, and exhibit increased epithelial density with an irregular, compact alveolar morphology observed

Strain of Origin: C57BL/6J X 129/Sv

Strain genetic background: C57BL/6J X 129/Sv

Strain Development: Chimeras derived from targeted mutations in Areg were mated to C57BL/6, and transmitting F1s were interbred or bred to C57BL/6. After several generations, homozygous nulls have been maintained by intercrossing for several years.

Strain Genetic Background Validation: A sample from this MMRRC strain was analyzed using the Mouse Universal Genotyping Array (MUGA) and MMRRC computational tools were used to assess the genetic background. A summary of the data can be found here.

MMRRC StrainReference StrainsGenomic makeup (Mb)
Strain Name MMRRC ID # Reference 1 Reference 2 Ref 1 Ref 2 Het Unknown
B6;129-Aregtm1Dle/Mmnc MMRRC:011533-UNC C57BL/6J 129S1/SvImJ 726 872 903 81


Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cancer
  • Cell Biology
  • Developmental Biology
  • Internal/Organ
  • Models for Human Disease
  • Neurobiology
  • Research tools
  • Immunology and Inflammation

Strain Origin

Donor: David C. Lee, Ph.D., University of North Carolina, Chapel Hill

Primary Reference:

  • Luetteke NC, Qiu TH, Fenton SE, Troyer KL, Riedel RF, Chang A, Lee DC. Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development. Development. 1999 Jun;126(12):2739-50. (Medline PMID: 10331984)
  • Sternlicht MD, Sunnarborg SW, Kouros-Mehr H, Yu Y, Lee DC, Werb Z. Mammary ductal morphogenesis requires paracrine activation of stromal EGFR via ADAM17-dependent shedding of epithelial amphiregulin. Development. 2005 Sep;132(17):3923-33. Epub 2005 Aug 3. Erratum in: Development. 2006 Mar;133(6):1203. (Medline PMID: 16079154)
  • Berasain C, Garcia-Trevijano ER, Castillo J, Erroba E, Lee DC, Prieto J, Avila MA. Amphiregulin: an early trigger of liver regeneration in mice. Gastroenterology. 2005 Feb;128(2):424-32. (Medline PMID: 15685553 )

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
011533-UNC-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
011533-UNC-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
011533-UNC-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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