Strain Name:
B6;129-Aregtm1Dle Egftm1Dle Tgfatm1Unc/Mmnc
Stock Number:
011536-UNC
Citation ID:
RRID:MMRRC_011536-UNC
Other Names:
epidermal growth factor, transforming growth factor-alpha 

Strain Information

Aregtm1Dle
Name: amphiregulin; targeted mutation 1, David C Lee
Synonyms: AR-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Knockout
Tgfatm1Unc
Name: transforming growth factor alpha; targeted mutation 1, University of North Carolina
Synonyms: TGFalpha-, Tgfatm1Dcl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Knockout
Egftm1Dle
Name: epidermal growth factor; targeted mutation 1, David C Lee
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Knockout
Areg
Name: amphiregulin
Synonyms: AR, Sdgf, Mcub
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Knockout
NCBI: 11839
HGNC: HGNC:651
Homologene: 1252
Tgfa
Name: transforming growth factor alpha
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Knockout
NCBI: 21802
Homologene: 2431
Egf
Name: epidermal growth factor
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Knockout
NCBI: 13645
HGNC: HGNC:3229
Homologene: 1483
Genetic Alterations
AREG: Exons 3 and 4 were replaced by a neo cassette. EGF: a neo cassette replaces exon 20 and surrounding intron sequences. TGFA: Exon 4 was replaced by a neo cassette and HSV-TK was inserted in reverse direction at the 3' end of exon 6.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    E14 derived from 129P2/OlaHsd
    Phenotype
    Homozygous: Appearance like TGFA-/-: Wavy hair, disorganized hair follicles, occasionally thinner adipose layer of skin. Penetrance of open eyes at birth and corneal scarring increased over TGFA-/-, also exhibits corneal ulceration, alopecia, and skin lesions in older animals.

    Heterozygous: Same as wild-type
    Mammalian Phenotype Terms
    Allelic Composition: Aregtm1Dle/Aregtm1Dle (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

    Allelic Composition: Tgfatm1Unc/Tgfatm1Unc (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

    Allelic Composition: Egftm1Dle/Egftm1Dle (Genetic Background: involves: 129P2/OlaHsd * C57BL/6J )

    MeSH Terms
    • Amphiregulin
    • Animals
    • Animals, Newborn
    • EGF Family of Proteins
    • Epidermal Growth Factor/genetics
    • Epidermal Growth Factor/metabolism
    • Female
    • Gene Expression Regulation, Developmental
    • Glycoproteins/genetics
    • Glycoproteins/metabolism
    • Growth Substances/genetics
    • Growth Substances/metabolism
    • Intercellular Signaling Peptides and Proteins
    • Lactation
    • Ligands
    • Mammary Glands, Animal/abnormalities
    • Mammary Glands, Animal/growth & development
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Morphogenesis/genetics
    • Mutation
    • Pregnancy
    • Receptor, Epidermal Growth Factor/metabolism
    • Survival Rate
    • Transcription, Genetic
    • Transforming Growth Factor alpha/genetics
    • Body Weight
    • Duodenal Diseases/pathology
    • Duodenum/growth & development
    • Epidermal Growth Factor/deficiency
    • Epidermal Growth Factor/physiology
    • Glycoproteins/deficiency
    • Glycoproteins/physiology
    • Growth Disorders/etiology
    • Growth Disorders/genetics
    • Growth Substances/deficiency
    • Growth Substances/physiology
    • Reverse Transcriptase Polymerase Chain Reaction
    • Species Specificity
    • Transforming Growth Factors/deficiency
    • Transforming Growth Factors/physiology
    Strain Development
    Chimeras derived from targeted mutations in Areg, Tgfa and Egf were separately mated to C57BL/6, and trasmitting F1s were interbred or bred to C57BL/6. After several generations, homozygous nulls for each gene were bred together until a triple null animal was created. Some of these were also recently bred to HB-EGF-/-, but are wild-type at this locus.
    Suggested Control Mice
    • Wildtype littermates
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Cell Biology
    • Dermatology
    • Developmental Biology
    • Immunology and Inflammation
    • Internal/Organ
    • Models for Human Disease
    • Research tools
    Donor
    David C. Lee, Ph.D., University of Georgia
    Primary Reference
    • Luetteke NC, Qiu TH, Fenton SE, Troyer KL, Riedel RF, Chang A, Lee DC. Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development. Development. 1999 Jun;126(12):2739-50. (Medline PMID: 10331984)
    • Troyer KL, Luetteke NC, Saxon ML, Qiu TH, Xian CJ, Lee DC. Growth retardation, duodenal lesions, and aberrant ileum architecture in triple null mice lacking EGF, amphiregulin, and TGF-alpha. Gastroenterology. 2001 Jul;121(1):68-78. (Medline PMID: 11438495)

    Colony and Husbandry Information

    Fetility is good but females rarely lactate well enough to support a litter. Pups must be cross-fostered, or a surrogate wild type female included in the breeding scheme to support pups.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    011536-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.