Strain Name:
B6;129-Areg+ Egf+ Tgfa+/Mmnc
Stock Number:
011537-UNC
Citation ID:
RRID:MMRRC_011537-UNC
Other Names:
epidermal growth factor, transforming growth factor-alpha 

Strain Information

Areg+
Name: amphiregulin; wild type
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Non-mutant
Egf+
Name: epidermal growth factor; wild type
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Non-mutant
Tgfa+
Name:
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Non-mutant
Areg
Name: amphiregulin
Synonyms: AR, Sdgf, Mcub
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Non-mutant
NCBI: 11839
HGNC: HGNC:651
Homologene: 1252
Tgfa
Name: transforming growth factor alpha
Type: Gene
Species: Mouse
Chromosome: 6
Alteration at locus: Non-mutant
NCBI: 21802
Homologene: 2431
Egf
Name: epidermal growth factor
Type: Gene
Species: Mouse
Chromosome: 3
Alteration at locus: Non-mutant
NCBI: 13645
HGNC: HGNC:3229
Homologene: 1483
Genetic Alterations
None
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • Phenotype
    Homozygous: wildtype

    Heterozygous: wildtype
    MeSH Terms
    • Amphiregulin
    • Animals
    • Animals, Newborn
    • EGF Family of Proteins
    • Epidermal Growth Factor/genetics
    • Epidermal Growth Factor/metabolism
    • Female
    • Gene Expression Regulation, Developmental
    • Glycoproteins/genetics
    • Glycoproteins/metabolism
    • Growth Substances/genetics
    • Growth Substances/metabolism
    • Intercellular Signaling Peptides and Proteins
    • Lactation
    • Ligands
    • Mammary Glands, Animal/abnormalities
    • Mammary Glands, Animal/growth & development
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Morphogenesis/genetics
    • Mutation
    • Pregnancy
    • Receptor, Epidermal Growth Factor/metabolism
    • Survival Rate
    • Transcription, Genetic
    • Transforming Growth Factor alpha/genetics
    • Body Weight
    • Duodenal Diseases/pathology
    • Duodenum/growth & development
    • Epidermal Growth Factor/deficiency
    • Epidermal Growth Factor/physiology
    • Glycoproteins/deficiency
    • Glycoproteins/physiology
    • Growth Disorders/etiology
    • Growth Disorders/genetics
    • Growth Substances/deficiency
    • Growth Substances/physiology
    • Reverse Transcriptase Polymerase Chain Reaction
    • Species Specificity
    • Transforming Growth Factors/deficiency
    • Transforming Growth Factors/physiology
    • Alanine Transaminase/blood
    • Apoptosis
    • Aspartate Aminotransferases/blood
    • Blotting, Western
    • Caspase 3
    • Caspases/metabolism
    • Cell Death
    • DNA-Binding Proteins/metabolism
    • Fas Ligand Protein
    • Gene Expression Regulation
    • Heparin-binding EGF-like Growth Factor
    • Hepatocytes/metabolism
    • In Situ Nick-End Labeling
    • Intercellular Signaling Peptides and Proteins/metabolism
    • Intercellular Signaling Peptides and Proteins/physiology
    • Interleukin-6/metabolism
    • Liver/injuries
    • Liver/metabolism
    • Liver/pathology
    • Male
    • Membrane Glycoproteins/biosynthesis
    • Mice, Transgenic
    • Proto-Oncogene Proteins c-bcl-2/biosynthesis
    • RNA/metabolism
    • RNA, Messenger/metabolism
    • STAT3 Transcription Factor
    • Time Factors
    • Trans-Activators/metabolism
    • Transforming Growth Factor alpha/biosynthesis
    • Up-Regulation
    • bcl-X Protein
    • fas Receptor/biosynthesis
    Strain Development
    Chimeras derived from targeted mutations in Areg, TGFa, and Egf were separately mated to C57BL/6, and transmitting F1s were interbred or bred to C57BL/6. After several generations, homozygous nulls for each gene were bred together to make multiple gene knockout lines. This line is a wild type control strain derived from these crosses, and serves as a control for AR-/-, EGF-/-, TGFA-/- and multiple knockout combinations of these genes.
    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Cancer
    • Cell Biology
    • Dermatology
    • Developmental Biology
    • Immunology and Inflammation
    • Internal/Organ
    • Models for Human Disease
    • Research Tools
    Donor
    David C. Lee, Ph.D., University of Georgia
    Primary Reference
    • Luetteke NC, Qiu TH, Fenton SE, Troyer KL, Riedel RF, Chang A, Lee DC. Targeted inactivation of the EGF and amphiregulin genes reveals distinct roles for EGF receptor ligands in mouse mammary gland development. Development. 1999 Jun;126(12):2739-50. (Medline PMID: 10331984)
    • Troyer KL, Luetteke NC, Saxon ML, Qiu TH, Xian CJ, Lee DC. Growth retardation, duodenal lesions, and aberrant ileum architecture in triple null mice lacking EGF, amphiregulin, and TGF-alpha. Gastroenterology. 2001 Jul;121(1):68-78. (Medline PMID: 11438495)
    • Berasain C, Garcia-Trevijano ER, Castillo J, Erroba E, Santamaria M, Lee DC, Prieto J, Avila MA. Novel role for amphiregulin in protection from liver injury. J Biol Chem. 2005 May 13;280(19):19012-20. Epub 2005 Mar 7. (Medline PMID: 15753092)

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    011537-UNC-EMBRYO Cryo-preserved embryos $1,404.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
    011537-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.